CARD Bait Capture README

The CARD Bait Capture Platform VERSION 1.0.0 is freely available under the Creative 
Commons CC-BY license version 4.0, https://creativecommons.org/licenses/by/4.0/

This bait set is based on CARD v1.0.1 reference sequences (December 14, 2015).

CITATION:

Guitor et al. "Capturing the Resistome: A  targeted capture method to reveal antibiotic 
resistance determinants in metagenomes" Antimicrobial Agents & Chemotherapy, 64: e01324-19.
https://www.ncbi.nlm.nih.gov/pubmed/31611361

FASTA:

AMR hybridization bait sequences described in Guitor et al. The header for each sequence
is as follows:

>P384758|gb|AF535087|ARO:3003047|qacB
	
where:

P384758				unique bait identifier
gb|AF535087			GenBank reference sequence used by CARD and during bait design
ARO:3003047			Antibiotic Resistance Ontology accession
qacB				gene name in CARD

PDF:

Suggested laboratory protocol for performing bait capture experiments.


