APH(3')-VI

Accession ARO:3003687
DefinitionAPH(3')-VI is an aminoglycoside phosphoryltransferase that acts on the 3-OH of target of aminoglycosides.
AMR Gene FamilyAPH(3')
Drug Classaminoglycoside antibiotic
Resistance Mechanismantibiotic inactivation
ResistomesAcinetobacter baumanniig+p+wgs, Acinetobacter haemolyticusp, Acinetobacter nosocomialisp+wgs, Citrobacter freundiiwgs, Enterobacter hormaecheiwgs, Escherichia colip+wgs, Klebsiella pneumoniaeg+p+wgs, Morganella morganiiwgs, Proteus mirabilisp+wgs, Providencia rettgeriwgs, Providencia stuartiiwgs, Pseudomonas aeruginosag+wgs, Pseudomonas fluorescenswgs, Salmonella entericap
Classification12 ontology terms | Show
Parent Term(s)8 ontology terms | Show
+ confers_resistance_to_antibiotic amikacin [Antibiotic]
+ confers_resistance_to_antibiotic ribostamycin [Antibiotic]
+ confers_resistance_to_antibiotic butirosin [Antibiotic]
+ confers_resistance_to_antibiotic kanamycin A [Antibiotic]
+ APH(3') [AMR Gene Family]
+ confers_resistance_to_antibiotic isepamicin [Antibiotic]
+ confers_resistance_to_antibiotic gentamicin B [Antibiotic]
+ confers_resistance_to_antibiotic paromomycin [Antibiotic]
Publications

Poole K, et al. 2005. Antimicrob. Agents Chemother. 49(2):479-87 Aminoglycoside resistance in Pseudomonas aeruginosa. (PMID 15673721)

Resistomes

Prevalence of APH(3')-VI among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 85 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
Acinetobacter baumannii0.64%1.16%0.78%
Acinetobacter haemolyticus0%6.67%0%
Acinetobacter lwoffii0%0%7.14%
Acinetobacter nosocomialis0%2%2%
Citrobacter freundii0%0%0.78%
Enterobacter cloacae0%0%0%
Enterobacter hormaechei0%0%0.48%
Escherichia coli0%0.03%0.05%
Klebsiella oxytoca0%0%0%
Klebsiella pneumoniae0.72%0.58%1.29%
Morganella morganii0%0%4.76%
Proteus mirabilis0%3.7%2.59%
Providencia rettgeri0%0%15.38%
Providencia stuartii0%0%7.69%
Pseudomonas aeruginosa1.4%0%0.49%
Pseudomonas fluorescens0%0%0.3%
Salmonella enterica0%0.12%0%
Serratia marcescens0%0%0.23%
Vibrio parahaemolyticus0%0%0.08%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 500


>gb|AGI04227.1|+|APH(3')-VI [Pseudomonas aeruginosa]
MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSSTLYTETTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFELMITKAI
NAKPISALFLTDQELLAIYKEALNLLNSVAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHRTYLSLWNELTETRVEER
LVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVERCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN


>gb|KC170992.1|+|8363-9142|APH(3')-VI [Pseudomonas aeruginosa]
ATGGAATTGCCCAATATTATTCAACAATTTATTGGAAACAGCGTTTTAGAGCCAAATAAAATTGGTCAGTCGCCATCGGATGTTTATTCT
TTTAATCGAAATAATGAAACTTTTTTTCTTAAGCGATCTAGCACTTTATATACAGAGACCACATACAGTGTCTCTCGCGAAGCGAAAATG
TTGAGTTGGCTCTCTGAGAAATTAAAGGTGCCTGAACTCATCATGACTTTTCAGGATGAGCAGTTTGAATTAATGATCACTAAAGCGATC
AATGCAAAACCAATTTCAGCGCTTTTTTTAACAGACCAAGAATTGCTTGCTATCTATAAGGAGGCACTCAATCTGTTAAATTCAGTTGCT
ATTATTGATTGTCCATTTATTTCAAACATTGATCATCGGTTAAAAGAGTCAAAATTTTTTATTGATAACCAACTCCTTGACGATATAGAT
CAAGATGATTTTGACGCTGAATTATGGGGAGACCATAGAACTTACCTAAGTCTATGGAATGAGTTAACTGAGACTCGTGTTGAAGAAAGA
TTGGTTTTTTCTCATGGCGATATCACGGATAGTAATATTTTTATAGATAAATTCAATGAAATTTACTTTTTAGATCTTGGCCGTGCTGGG
TTAGCTGATGAATTTGTAGATATATCCTTTGTTGAACGTTGCCTAAGAGAGGATGCCTCGGAGGAAACTGCTAAAATATTTTTAAAGCAT
TTAAAAAATGATAGACCTGACAAAAGGAATTATTTTTTAAAACTTGATGAATTGAATTGA