| Accession | ARO:3003754 |
| Definition | The nfsA-encoded nitroreductase is the major oxygen-insensitive nitroreductase present in E. coli. NfsA uses only NADPH and has broad electron acceptor specificity. Mutations in nfsA cause resistance to nitrofurazone and furazolidone. Resistance to nitrofurantoin via mutation of nfsA reduces the fitness of clinical isolates of E. coli. |
| Drug Class | nitrofuran antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Classification | 6 ontology terms | Show |
| Parent Term(s) | 2 ontology terms | Show + antibiotic resistant gene variant or mutant + confers_resistance_to_drug_class nitrofuran antibiotic [Drug Class] |
| Sub-Term(s) | 1 ontology terms | Show |
| Publications | Sandegren L, et al. 2008. J. Antimicrob. Chemother. 62(3):495-503 Nitrofurantoin resistance mechanism and fitness cost in Escherichia coli. (PMID 18544599) Race PR, et al. 2005. J. Biol. Chem. 280(14):13256-64 Structural and mechanistic studies of Escherichia coli nitroreductase with the antibiotic nitrofurazone. Reversed binding orientations in different redox states of the enzyme. (PMID 15684426) Whiteway J, et al. 1998. J. Bacteriol. 180(21):5529-39 Oxygen-insensitive nitroreductases: analysis of the roles of nfsA and nfsB in development of resistance to 5-nitrofuran derivatives in Escherichia coli. (PMID 9791100) McCalla DR, et al. 1978. J. Bacteriol. 133(1):10-6 Genetics of nitrofurazone resistance in Escherichia coli. (PMID 338576) |