| Accession | ARO:3003889 |
| CARD Short Name | Ecol_GlpT_FOF |
| Definition | Point mutations to the active importer GlpT, which is involved with the uptake of many phosphorylated sugars, confer resistance to fosfomycin by reducing import of the drug into the bacteria. |
| AMR Gene Family | antibiotic-resistant GlpT |
| Drug Class | phosphonic acid antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Resistomes with Sequence Variants | Citrobacter amalonaticusg+wgs, Citrobacter freundiig+p+wgs, Citrobacter koserig+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniig+wgs, Citrobacter youngaeg+wgs, Cronobacter condimentig+wgs, Cronobacter dublinensisg+wgs, Cronobacter malonaticusg+wgs, Cronobacter sakazakiig+wgs, Cronobacter turicensiswgs, Cronobacter universalisg+wgs, Edwardsiella tardag+wgs, Enterobacter cloacaewgs, Enterobacter hormaecheiwgs, Enterobacter kobeiwgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs, Escherichia fergusoniig+wgs, Escherichia marmotaeg+wgs, Salmonella bongorig+wgs, Salmonella entericag+wgs, Serratia marcescensg+wgs, Serratia rubidaeag+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Classification | 8 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + mutation conferring antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + phosphonic acid antibiotic [Drug Class] + antibiotic resistant gene variant or mutant |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic fosfomycin [Antibiotic] + antibiotic resistant gene variant or mutant + antibiotic-resistant GlpT [AMR Gene Family] |
| Publications | Takahata S, et al. 2010. Int J Antimicrob Agents 35(4): 333-337. Molecular mechanisms of fosfomycin resistance in clinical isolates of Escherichia coli. (PMID 20071153) Nilsson AI, et al. 2003. Antimicrob Agents Chemother 47(9): 2850-2858. Biological costs and mechanisms of fosfomycin resistance in Escherichia coli. (PMID 12936984) |
Prevalence of Escherichia coli GlpT with mutation conferring resistance to fosfomycin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Citrobacter amalonaticus | 72.73% | 0% | 72.73% | 0% |
| Citrobacter freundii | 90.16% | 0.31% | 48.36% | 0% |
| Citrobacter koseri | 100% | 0% | 47.75% | 0% |
| Citrobacter portucalensis | 74.07% | 0% | 59.46% | 0% |
| Citrobacter werkmanii | 100% | 0% | 61.54% | 0% |
| Citrobacter youngae | 66.67% | 0% | 100% | 0% |
| Cronobacter condimenti | 100% | 0% | 100% | 0% |
| Cronobacter dublinensis | 100% | 0% | 97.44% | 0% |
| Cronobacter malonaticus | 100% | 0% | 85.45% | 0% |
| Cronobacter sakazakii | 100% | 0% | 91.7% | 0% |
| Cronobacter turicensis | 0% | 0% | 75% | 0% |
| Cronobacter universalis | 100% | 0% | 100% | 0% |
| Edwardsiella tarda | 30% | 0% | 13.33% | 0% |
| Enterobacter cloacae | 0% | 0% | 0.32% | 0% |
| Enterobacter hormaechei | 0% | 0% | 0.04% | 0% |
| Enterobacter kobei | 0% | 0% | 0.44% | 0% |
| Escherichia albertii | 100% | 0% | 62.58% | 0% |
| Escherichia coli | 52.22% | 0.04% | 53.61% | 0% |
| Escherichia fergusonii | 100% | 0% | 51.09% | 0% |
| Escherichia marmotae | 100% | 0% | 70.83% | 0% |
| Salmonella bongori | 100% | 0% | 94.74% | 0% |
| Salmonella enterica | 94.83% | 0% | 80.3% | 0% |
| Serratia marcescens | 27.27% | 0% | 20.45% | 0% |
| Serratia rubidaea | 100% | 0% | 90.91% | 0% |
| Shigella boydii | 86.67% | 0% | 91.11% | 0% |
| Shigella dysenteriae | 100% | 0% | 96.67% | 0% |
| Shigella flexneri | 94% | 0% | 81.83% | 0% |
| Shigella sonnei | 100% | 0% | 91.89% | 0% |
Model Type: protein variant model
Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 850
Legend:
Published Variants:
| PMID: 20071153 | E448K E448K,G302D E448K,G33R E448K,Q444E,E443Q,L297F -nt602:20 |