| Accession | ARO:3000216 |
| Synonym(s) | ECK0456 JW0451 |
| CARD Short Name | acrB |
| Definition | Protein subunit of AcrA-AcrB-TolC multidrug efflux complex. AcrB functions as a herterotrimer which forms the inner membrane component and is primarily responsible for substrate recognition and energy transduction by acting as a drug/proton antiporter. |
| AMR Gene Family | resistance-nodulation-cell division (RND) antibiotic efflux pump |
| Drug Class | tetracycline antibiotic, disinfecting agents and antiseptics, phenicol antibiotic, rifamycin antibiotic, penam, glycylcycline, cephalosporin, fluoroquinolone antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Perfect Matches | Escherichia colig+p+wgs+gi, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Resistomes with Sequence Variants | Citrobacter amalonaticusg+wgs, Citrobacter freundiig+p+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniig+wgs, Citrobacter youngaeg+wgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniig+p+wgs, Escherichia marmotaeg+wgs+gi, Salmonella bongorig+wgs, Salmonella entericag+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Classification | 24 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + beta-lactam antibiotic + mechanism of antibiotic resistance + cephem + tetracycline antibiotic [Drug Class] + determinant of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + disinfecting agents and antiseptics [Drug Class] + phenicol antibiotic [Drug Class] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + rifamycin antibiotic [Drug Class] + penam [Drug Class] + glycylcycline [Drug Class] + cephalosporin [Drug Class] + triclosan [Antibiotic] + cefalotin [Antibiotic] + ampicillin [Antibiotic] + chloramphenicol [Antibiotic] + rifampin [Antibiotic] + resistance-nodulation-cell division (RND) antibiotic efflux pump [AMR Gene Family] + tetracycline [Antibiotic] + tigecycline [Antibiotic] + fluoroquinolone antibiotic [Drug Class] |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic acriflavine [Antibiotic] + part_of AcrAB-TolC + subunit of efflux pump conferring antibiotic resistance |
| Publications | Tornroth-Horsefield S, et al. 2007. Structure 15(12): 1663-1673. Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist. (PMID 18073115) Murakami S, et al. 2002. Nature 419(6907): 587-593. Crystal structure of bacterial multidrug efflux transporter AcrB. (PMID 12374972) Das D, et al. 2007. J Struct Biol 158(3): 494-502. Crystal structure of the multidrug efflux transporter AcrB at 3.1A resolution reveals the N-terminal region with conserved amino acids. (PMID 17275331) Pos KM. 2009. Biochim Biophys Acta 1794(5): 782-793. Drug transport mechanism of the AcrB efflux pump. (PMID 19166984) Eicher T, et al. 2009. Biol Chem 390(8): 693-699. Structural and functional aspects of the multidrug efflux pump AcrB. (PMID 19453279) |
Prevalence of acrB among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Citrobacter amalonaticus | 90.91% | 0% | 74.55% | 0% |
| Citrobacter freundii | 75.41% | 0.31% | 48.74% | 0% |
| Citrobacter portucalensis | 55.56% | 0% | 15.32% | 0% |
| Citrobacter werkmanii | 71.43% | 0% | 58.97% | 0% |
| Citrobacter youngae | 66.67% | 0% | 87.5% | 0% |
| Escherichia albertii | 100% | 0% | 62.58% | 0% |
| Escherichia coli | 67.7% | 0.01% | 61.14% | 3.19% |
| Escherichia fergusonii | 100% | 0.36% | 50% | 0% |
| Escherichia marmotae | 100% | 0% | 70.83% | 50% |
| Salmonella bongori | 100% | 0% | 94.74% | 0% |
| Salmonella enterica | 85.05% | 0% | 73.41% | 0% |
| Shigella boydii | 100% | 0% | 96.67% | 0% |
| Shigella dysenteriae | 92.86% | 0% | 100% | 0% |
| Shigella flexneri | 99% | 0% | 81.99% | 0% |
| Shigella sonnei | 100% | 0% | 92.99% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 1900
