| Accession | ARO:3000830 |
| CARD Short Name | cpxA |
| Definition | CpxA is a membrane-localized sensor kinase that is activated by envelope stress. It starts a kinase cascade that activates CpxR, which promotes efflux complex expression. |
| AMR Gene Family | resistance-nodulation-cell division (RND) antibiotic efflux pump |
| Drug Class | aminoglycoside antibiotic, aminocoumarin antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Efflux Regulator | protein(s) and two-component regulatory system modulating antibiotic efflux |
| Resistomes with Perfect Matches | Escherichia colig+p+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Resistomes with Sequence Variants | Escherichia colig+p+wgs+gi, Salmonella entericagi, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Classification | 20 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic molecule + determinant of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + antibiotic mixture + aminoglycoside antibiotic [Drug Class] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + aminocoumarin antibiotic [Drug Class] + novobiocin [Antibiotic] + tobramycin [Antibiotic] + kanamycin A [Antibiotic] + neomycin [Antibiotic] + resistance-nodulation-cell division (RND) antibiotic efflux pump [AMR Gene Family] + amikacin [Antibiotic] + gentamicin [Antibiotic] + protein(s) and two-component regulatory system modulating antibiotic efflux [Efflux Regulator] + MdtABC-TolC + AcrAD-TolC + acrD |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Srinivasan VB, et al. 2012. PLoS One 7(4): E33777. Role of the two component signal transduction system CpxAR in conferring cefepime and chloramphenicol resistance in Klebsiella pneumoniae NTUH-K2044. (PMID 22496764) |
Prevalence of cpxA among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Escherichia coli | 60.53% | 0.01% | 54.86% | 0.13% |
| Salmonella enterica | 0% | 0% | 0% | 5.63% |
| Shigella boydii | 93.33% | 0% | 86.67% | 0% |
| Shigella dysenteriae | 21.43% | 0% | 63.33% | 0% |
| Shigella flexneri | 59% | 0% | 11.34% | 0% |
| Shigella sonnei | 100% | 0% | 94.81% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 890