RGI Bulk Analysis: Do you want to use RGI to analyze a large number of genomes? It is now available as a downloadable command-line tool in the Download section of the CARD website.
RGI 4.0.0: Open Reading Frame (ORF) prediction using Prodigal, homolog detection using Diamond, and Strict significance based on CARD curated bitscore cut-offs. Addition of rRNA mutation and efflux over-expression models. Hits of 95% identity or better are automatically listed as Strict. All results organized by revised ARO classification: AMR Gene Family, Drug Class, and Resistance Mechanism.
Online RGI results cached for 7 days. As the CARD curation evolves, the results of the RGI evolve. RGI targets, reference sequences, and significance cut-offs are under constant curation.
The RGI analyzes sequences under three paradigms – Perfect, Strict, and Loose (a.k.a. Discovery). The Perfect algorithm is most often applied to clinical surveillance as it detects perfect matches to the curated reference sequences and mutations in the CARD. In contrast, the Strict algorithm detects previously unknown variants of known AMR genes, including secondary screen for key mutations, using detection models with curated similarity cut-offs to ensure the detected variant is likely a functional AMR gene. The Loose algorithm works outside of the detection model cut-offs to provide detection of new, emergent threats and more distant homologs of AMR genes, but will also catalog homologous sequences and spurious partial hits that may not have a role in AMR. Combined with phenotypic screening, the Loose algorithm allows researchers to hone in on new AMR genes.
Upload a JSON file containing RGI results generated using the command-line version. File size limited to 20 Mb. Note that only Loose hits of e-10 or better can be visualized.