| Accession | ARO:3000149 |
| CARD Short Name | FosA |
| Definition | An enzyme that confers resistance to fosfomycin in Serratia marcescens by breaking the epoxide ring of the molecule. It depends on the cofactors Manganese (II) and Potassium and uses Glutathione (GSH) as the nucleophilic molecule. In Pseudomonas aeruginosa, FosA catalyzes the conjugation of glutathione to carbon-1 of fosfomycin, rendering it ineffective as an antibacterial drug. |
| AMR Gene Family | fosfomycin thiol transferase |
| Drug Class | phosphonic acid antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Pseudomonas aeruginosag+p+wgs |
| Resistomes with Sequence Variants | Pseudomonas aeruginosag+p+wgs, Pseudomonas chlororaphiswgs, Pseudomonas fluorescensg+wgs, Pseudomonas koreensisg+wgs, Pseudomonas putidawgs, Pseudomonas syringaeg+wgs |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + determinant of antibiotic resistance + antibiotic inactivation enzyme + antibiotic molecule + hydrolysis of antibiotic conferring resistance + phosphonic acid antibiotic [Drug Class] + hydrolysis of fosfomycin epoxide ring + fosfomycin inactivation enzyme |
| Parent Term(s) | 2 ontology terms | Show + fosfomycin thiol transferase [AMR Gene Family] + confers_resistance_to_antibiotic fosfomycin [Antibiotic] |
| Sub-Term(s) | 3 ontology terms | Show + phosphonoformate [Adjuvant] is_small_molecule_inhibitor + phosphonoacetate [Adjuvant] is_small_molecule_inhibitor + acetylphosphonate [Adjuvant] is_small_molecule_inhibitor |
| Publications | Pakhomova S, et al. 2004. Protein Sci 13(5): 1260-1265. Structure of fosfomycin resistance protein FosA from transposon Tn2921. (PMID 15075406) Beharry Z, et al. 2005. J. Biol. Chem. 280(18):17786-91 Functional analysis of active site residues of the fosfomycin resistance enzyme FosA from Pseudomonas aeruginosa. (PMID 15741169) |
Prevalence of FosA among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Pseudomonas aeruginosa | 99.08% | 0.29% | 67.57% | 0% |
| Pseudomonas chlororaphis | 0% | 0% | 3.23% | 0% |
| Pseudomonas fluorescens | 27.78% | 0% | 9.13% | 0% |
| Pseudomonas koreensis | 75% | 0% | 56.52% | 0% |
| Pseudomonas putida | 0% | 0% | 1.07% | 0% |
| Pseudomonas syringae | 2.08% | 0% | 0.34% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 200
