Accession ARO:3000198
CARD Short NameFosX
DefinitionFosX is an enzyme used to confer resistance to fosfomycin. It's dependent on the cofactor, manganese (II), and uses water to generate a vicinal diol.
AMR Gene Familyfosfomycin thiol transferase
Drug Classphosphonic acid antibiotic
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesListeria monocytogenesg+p+wgs
Resistomes with Sequence VariantsListeria innocuag+wgs, Listeria monocytogenesg+p+wgs
Classification10 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ fosfomycin thiol transferase [AMR Gene Family]
+ confers_resistance_to_antibiotic fosfomycin [Antibiotic]

Fillgrove KL, et al. 2007. Biochemistry 46(27): 8110-8120. Structure and mechanism of the genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes. (PMID 17567049)


Prevalence of FosX among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Listeria innocua100%0%39.85%0%
Listeria monocytogenes99.16%0.97%74.47%0%
Show Perfect Only

Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 200

>gb|CWV56762.1|+|FosX [Listeria monocytogenes]

>gb|FFFO01000009.1|+|81761-82162|FosX [Listeria monocytogenes]