Accession ARO:3000198
DefinitionFosX is an enzyme used to confer resistance to fosfomycin. It's dependent on the cofactor, manganese (II), and uses water to generate a vicinal diol.
AMR Gene Familyfosfomycin thiol transferase
Drug Classfosfomycin
Resistance Mechanismantibiotic inactivation
ResistomesListeria monocytogenesg+wgs
Classification12 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic fosfomycin [Drug Class]
+ fosfomycin thiol transferase [AMR Gene Family]
Publications

Fillgrove KL, et al. 2007. Biochemistry 46(27): 8110-8120. Structure and mechanism of the genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes. (PMID 17567049)

Resistomes

Prevalence of FosX among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 85 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
Listeria monocytogenes99.51%0.68%99.63%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 200


>gb|CWV56762.1|+|FosX [Listeria monocytogenes]
MISGLSHITLIVKDLNKTTAFLQNIFNAEEIYSSGDKTFSLSKEKFFLIAGLWICIMEGDSLQERTYNHIAFQIQSEEVDEYTERIKALG
VEMKPERPRVQGEGRSIYFYDFDNHLFELHAGTLEERLKRYHE


>gb|FFFO01000009.1|+|81761-82162|FosX [Listeria monocytogenes]
ATGATTTCAGGATTAAGCCATATCACTTTAATTGTGAAAGATTTGAATAAAACAACTGCTTTCTTACAGAATATTTTTAATGCAGAAGAA
ATCTATTCTAGTGGCGACAAAACATTTTCGCTTTCCAAAGAAAAATTTTTTCTAATAGCTGGTTTGTGGATTTGCATTATGGAAGGAGAT
TCTTTACAAGAGCGAACTTACAATCATATTGCTTTCCAAATTCAATCCGAGGAAGTGGATGAATATACTGAGCGGATTAAAGCTCTCGGT
GTGGAAATGAAACCAGAACGTCCCAGAGTCCAAGGTGAAGGACGTTCCATTTATTTTTATGATTTTGATAATCATCTGTTTGAATTACAC
GCTGGTACATTAGAAGAACGCTTAAAAAGGTATCACGAATAG