| Accession | ARO:3000198 |
| CARD Short Name | FosX |
| Definition | FosX is an enzyme used to confer resistance to fosfomycin. It's dependent on the cofactor, manganese (II), and uses water to generate a vicinal diol. |
| AMR Gene Family | fosfomycin thiol transferase |
| Drug Class | phosphonic acid antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Listeria monocytogenesg+p+wgs |
| Resistomes with Sequence Variants | Listeria innocuag+wgs, Listeria monocytogenesg+p+wgs |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + determinant of antibiotic resistance + antibiotic inactivation enzyme + antibiotic molecule + hydrolysis of antibiotic conferring resistance + hydrolysis of fosfomycin epoxide ring + fosfomycin inactivation enzyme + phosphonic acid antibiotic [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show + fosfomycin thiol transferase [AMR Gene Family] + confers_resistance_to_antibiotic fosfomycin [Antibiotic] |
| Publications | Fillgrove KL, et al. 2007. Biochemistry 46(27): 8110-8120. Structure and mechanism of the genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes. (PMID 17567049) |
Prevalence of FosX among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Listeria innocua | 100% | 0% | 39.85% | 0% |
| Listeria monocytogenes | 99.16% | 0.97% | 74.47% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 200
