| Accession | ARO:3003755 |
| Definition | The nsfB gene encodes a minor oxygen-insensitive nitroreductase. NfsB reduces a broad range of nitroaromatic compounds including the antibiotics nitrofurazone and nitrofurantoin. NfsB is a flavin mononucleotide (FMN)-containing protein and uses both NADH and NADPH as a source of reducing equivalents. Mutations in nfsB lead to increased resistance to nitrofurazone and furazolidone in an nfsA mutant background. |
| Drug Class | nitrofuran antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Classification | 6 ontology terms | Show |
| Parent Term(s) | 2 ontology terms | Show + antibiotic resistant gene variant or mutant + confers_resistance_to_drug_class nitrofuran antibiotic [Drug Class] |
| Sub-Term(s) | 1 ontology terms | Show |
| Publications | McCalla DR, et al. 1978. J. Bacteriol. 133(1):10-6 Genetics of nitrofurazone resistance in Escherichia coli. (PMID 338576) Bryant DW, et al. 1981. Can. J. Microbiol. 27(1):81-6 Type I nitroreductases of Escherichia coli. (PMID 7011517) Whiteway J, et al. 1998. J. Bacteriol. 180(21):5529-39 Oxygen-insensitive nitroreductases: analysis of the roles of nfsA and nfsB in development of resistance to 5-nitrofuran derivatives in Escherichia coli. (PMID 9791100) Zenno S, et al. 1996. J. Biochem. 120(4):736-44 Gene cloning, purification, and characterization of NfsB, a minor oxygen-insensitive nitroreductase from Escherichia coli, similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri. (PMID 8947835) |