| Accession | ARO:3003756 |
| CARD Short Name | Ecol_nfsB_NIT |
| Definition | nfsB encodes the minor oxygen-insesitive nitroreductase in E. coli. The first step of resistance to nitrofurazone is mutation of nfsA, while the increased resistance associated with second step mutants is a consequence of nfsB mutation. |
| AMR Gene Family | Antibiotic resistant nfsB |
| Drug Class | nitrofuran antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Classification | 8 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + mutation conferring antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + nitrofuran antibiotic [Drug Class] + antibiotic resistant gene variant or mutant |
| Parent Term(s) | 2 ontology terms | Show + confers_resistance_to_antibiotic nitrofurantoin [Antibiotic] + Antibiotic resistant nfsB [AMR Gene Family] |
| Publications | Whiteway J, et al. 1998. J. Bacteriol. 180(21):5529-39 Oxygen-insensitive nitroreductases: analysis of the roles of nfsA and nfsB in development of resistance to 5-nitrofuran derivatives in Escherichia coli. (PMID 9791100) Sandegren L, et al. 2008. J. Antimicrob. Chemother. 62(3):495-503 Nitrofurantoin resistance mechanism and fitness cost in Escherichia coli. (PMID 18544599) Ho PL, et al. 2015. Antimicrob. Agents Chemother. 60(1):537-43 Plasmid-Mediated OqxAB Is an Important Mechanism for Nitrofurantoin Resistance in Escherichia coli. (PMID 26552976) |
Prevalence of Escherichia coli nfsB with mutation conferring resistance to nitrofurantoin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| No prevalence data | |||||
Model Type: protein variant model
Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 400
PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).| Mutation | Mutation type | PubMed |
|---|---|---|
| E137Ter | nonsense mutation | PMID:26552976 |
| 40407,A119fs+40413,Y183Ter | co-dependent nonsense SNP | PMID:18544599 |
| 40407,A119fs+40413,Q44L | co-dependent single resistance variant | PMID:18544599 |
| 40407,A119fs+40413,del79-93/nt229-44 | co-dependent insertion/deletion | PMID:18544599 |
| 40407,A119fs+40413,G116D | co-dependent single resistance variant | PMID:18544599 |
| 40407,A119fs+40413,V93G | co-dependent single resistance variant | PMID:18544599 |
| 40407,A119fs+40413,N211fs/nt635-1:T | co-dependent insertion/deletion | PMID:18544599 |
| 40407,N134fs+40413,A156fs/nt466-1:G | co-dependent insertion/deletion | PMID:18544599 |
| 40407,N134fs+40413,G182fs/nt545-2 | co-dependent insertion/deletion | PMID:18544599 |
| 40407,A119fs+40413,S43fs/nt130-1:C | co-dependent insertion/deletion | PMID:18544599 |
| 40407,N134fs+40413,W94Ter | co-dependent nonsense SNP | PMID:18544599 |
| 40407,A119fs+40413,G192A | co-dependent single resistance variant | PMID:18544599 |
| 40407,N134fs+40413,W138C | co-dependent single resistance variant | PMID:18544599 |
| 40407,A119fs+40413,R107H | co-dependent single resistance variant | PMID:18544599 |
| 40407,N134fs+40413,E165A | co-dependent single resistance variant | PMID:18544599 |
| 40407,N134fs+40413,G192D | co-dependent single resistance variant | PMID:18544599 |
| 40407,N134fs+40413,del86-112/nt259-80 | co-dependent insertion/deletion | PMID:18544599 |
| 40407,N134fs+40413,Q101Ter | co-dependent nonsense SNP | PMID:18544599 |
| 40407,A119fs+40413,K74fs/nt223-1:A | co-dependent insertion/deletion | PMID:18544599 |
| 40407,N134fs+40413,del102-157/nt303-167 | co-dependent insertion/deletion | PMID:18544599 |
| Curator | Description | Most Recent Edit |
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