Staphylococcus aureus fusA with mutation conferring resistance to fusidic acid

Download Sequences
Accession ARO:3003735
CARD Short NameSaur_fusA_FA
DefinitionThe mutations to this gene are involved in altering the translation elongation factor G (EF-G) in association with the ribosome to prevent fusidic acid from binding EF-G and preventing translation.
AMR Gene Familyantibiotic resistant fusA
Drug Classfusidane antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Sequence VariantsStaphylococcus aureusg+wgs
Classification8 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic fusidic acid [Antibiotic]
+ antibiotic resistant fusA [AMR Gene Family]
Publications

Lannergard J, et al. 2009. Antimicrob Agents Chemother 53(5): 2059-2065. Genetic determinants of resistance to fusidic acid among clinical bacteremia isolates of Staphylococcus aureus. (PMID 19289529)

Castanheira M, et al. 2010. Antimicrob. Agents Chemother. 54(9):3614-7 Fusidic acid resistance rates and prevalence of resistance mechanisms among Staphylococcus spp. isolated in North America and Australia, 2007-2008. (PMID 20566766)

Castanheira M, et al. 2010. J. Antimicrob. Chemother. 65(7):1353-8 Occurrence and molecular characterization of fusidic acid resistance mechanisms among Staphylococcus spp. from European countries (2008). (PMID 20430787)

Nagaev I, et al. 2001. Mol. Microbiol. 40(2):433-9 Biological cost and compensatory evolution in fusidic acid-resistant Staphylococcus aureus. (PMID 11309125)

Besier S, et al. 2003. Mol. Microbiol. 47(2):463-9 Molecular analysis of fusidic acid resistance in Staphylococcus aureus. (PMID 12519196)

Frosini SM, et al. 2020. Microorganisms 8(12): Genes on the Move: In Vitro Transduction of Antimicrobial Resistance Genes between Human and Canine Staphylococcal Pathogens. (PMID 33353175)
Resistomes

Prevalence of Staphylococcus aureus fusA with mutation conferring resistance to fusidic acid among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GIGRDI-AMR2
Staphylococcus aureus1.92%0%1.8%0%0%
Show Perfect Only


Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 1350

PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).

MutationMutation typePubMed
A67T,P406Lmultiple resistance variantsPMID:12519196
A70V,A160V,H457Ymultiple resistance variantsPMID:20430787
F88Lsingle resistance variantPMID:11309125
V90Asingle resistance variantPMID:20430787
V90Isingle resistance variantPMID:20430787
V90I,H457Q,L461K,A655Vmultiple resistance variantsPMID:12519196
Q115Lsingle resistance variantPMID:11309125
D189V,L430Smultiple resistance variantsPMID:20430787
A376Vsingle resistance variantPMID:20430787
T387I,E449Kmultiple resistance variantsPMID:20430787
P404Qsingle resistance variantPMID:11309125
P404Lsingle resistance variantPMID:20430787
P406Lsingle resistance variantPMID:12519196
V407Fsingle resistance variantPMID:11309125
D434Nsingle resistance variantPMID:11309125
T436Isingle resistance variantPMID:11309125
F441Ysingle resistance variantPMID:20430787
G451Vsingle resistance variantPMID:11309125
G452Ssingle resistance variantPMID:11309125
G452Csingle resistance variantPMID:11309125
G452C,R659Lmultiple resistance variantsPMID:11309125
G452Vsingle resistance variantPMID:11309125
M453Isingle resistance variantPMID:20566766
L456Fsingle resistance variantPMID:11309125
H457Lsingle resistance variantPMID:33353175
H457Ysingle resistance variantPMID:12519196
H457Qsingle resistance variantPMID:20566766
H457Y,S416Fmultiple resistance variantsPMID:12519196
H457Nsingle resistance variantPMID:33353175
L461Fsingle resistance variantPMID:12519196
L461Ssingle resistance variantPMID:20430787
L461Ksingle resistance variantPMID:12519196
R464Lsingle resistance variantPMID:11309125
R464Hsingle resistance variantPMID:11309125
R464Csingle resistance variantPMID:11309125
R464Ssingle resistance variantPMID:11309125
F652S,Y654Nmultiple resistance variantsPMID:12519196

>gb|CAG39573.1|+|Staphylococcus aureus fusA with mutation conferring resistance to fusidic acid [Staphylococcus aureus subsp. aureus MRSA252]
MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRG
ITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVW
RQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVE
MKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSEL
KEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVI
AKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHA
NSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQ
DKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMV
SYRETFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPS
VEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVI
LEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRS
NTQGRGTYTMYFDHYAEVPKSIAEDIIKKNKGE


>gb|BX571856.1|+|599703-601784|Staphylococcus aureus fusA with mutation conferring resistance to fusidic acid [Staphylococcus aureus subsp. aureus MRSA252]
ATGGCTAGAGAATTTTCATTAGAAAAAACTCGTAATATCGGTATCATGGCTCACATTGATGCTGGTAAAACGACTACGACTGAACGTATT
CTTTATTACACTGGCCGTATCCACAAAATTGGTGAAACACATGAAGGTGCTTCACAAATGGACTGGATGGAGCAAGAACAAGACCGTGGT
ATTACTATCACATCTGCTGCAACAACAGCAGCTTGGGAAGGTCACCGTGTAAACATTATCGATACACCTGGACACGTAGACTTCACTGTA
GAAGTTGAACGTTCATTACGTGTACTTGACGGAGCAGTTACAGTACTTGATGCACAATCAGGTGTTGAACCTCAAACTGAAACAGTTTGG
CGTCAGGCTACAACTTATGGTGTTCCACGTATCGTATTTGTAAACAAAATGGACAAATTAGGTGCTAACTTCGAATACTCTGTAAGTACA
TTACATGATCGTTTACAAGCTAACGCTGCTCCAATCCAATTACCAATTGGTGCGGAAGACGAATTCGAAGCAATCATTGACTTAGTTGAA
ATGAAATGTTTCAAATATACAAATGATTTAGGTACTGAAATTGAAGAAATTGAAATTCCTGAAGACCACTTAGATAGAGCTGAAGAAGCT
CGTGCTAGCTTAATCGAAGCAGTTGCAGAAACTAGCGACGAATTAATGGAAAAATATCTTGGTGACGAAGAAATTTCAGTTTCTGAATTA
AAAGAAGCTATCCGCCAAGCTACTACTAACGTAGAATTCTACCCAGTACTTTGTGGTACAGCTTTCAAAAACAAAGGTGTTCAATTAATG
CTTGACGCTGTAATTGATTACTTACCTTCACCACTAGACGTTAAACCAATTATTGGTCACCGTGCTAGCAACCCTGAAGAAGAAGTAATC
GCGAAAGCAGACGATTCAGCTGAATTCGCTGCATTAGCGTTCAAAGTTATGACTGACCCTTATGTTGGTAAATTAACATTCTTCCGTGTG
TACTCAGGTACAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCT
AACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGT
GGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCAA
GATAAAATGACTCAAGCTTTAGTTAAATTACAAGAAGAAGACCCAACATTCCATGCACACACTGACGAAGAAACTGGACAAGTTATCATC
GGTGGTATGGGTGAGCTTCACTTAGACATCTTAGTAGACCGTATGAAGAAAGAATTCAACGTTGAATGTAACGTAGGTGCTCCAATGGTT
TCATATCGTGAAACATTCAAATCATCTGCACAAGTTCAAGGTAAATTCTCTCGTCAATCTGGTGGTCGTGGTCAATACGGTGATGTTCAC
ATTGAATTCACACCAAACGAAACAGGCGCAGGTTTCGAATTCGAAAACGCTATCGTTGGTGGTGTAGTTCCTCGTGAATACATTCCATCA
GTAGAAGCTGGTCTTAAAGATGCTATGGAAAATGGTGTCTTAGCAGGTTATCCATTAATTGATGTTAAAGCTAAATTATATGATGGTTCA
TACCATGATGTCGATTCATCTGAAATGGCCTTCAAAATTGCTGCATCATTAGCACTTAAAGAAGCTGCTAAAAAATGTGATCCTGTAATC
TTAGAACCAATGATGAAAGTAACTATTGAAATGCCTGAAGAGTACATGGGTGATATCATGGGTGACGTAACATCTCGTCGTGGACGTGTT
GATGGTATGGAACCTCGTGGTAATGCACAAGTTGTTAATGCTTATGTACCACTTTCAGAAATGTTCGGTTATGCAACATCATTACGTTCA
AACACTCAAGGTCGCGGTACTTACACTATGTACTTCGATCACTATGCTGAAGTTCCAAAATCAATCGCTGAAGATATTATCAAGAAAAAT
AAAGGTGAATAA

Curator Acknowledgements
Curator Description Most Recent Edit