Borreliella burgdorferi murA with mutation conferring resistance to fosfomycin

Accession ARO:3003777
CARD Short NameBbur_mur_FOF
DefinitionmurA or UDP-N-acetylglucosamine enolpyruvyl transferase catalyses the initial step in peptidoglycan biosynthesis and is inhibited by fosfomycin. Mutations to the murA enzyme confers resistance to the antibiotic.
AMR Gene Familyantibiotic-resistant murA transferase
Drug Classphosphonic acid antibiotic
Resistance Mechanismantibiotic target alteration
Classification8 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic fosfomycin [Antibiotic]
+ antibiotic-resistant murA transferase [AMR Gene Family]
Publications

Jiang S, et al. 2011. Biochemistry 50(12):2205-12 Lyme disease enolpyruvyl-UDP-GlcNAc synthase: fosfomycin-resistant MurA from Borrelia burgdorferi, a fosfomycin-sensitive mutant, and the catalytic role of the active site Asp. (PMID 21294548)

Resistomes

Prevalence of Borreliella burgdorferi murA with mutation conferring resistance to fosfomycin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
No prevalence data


Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 800

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:


>gb|AAC66824.2|+|Borreliella burgdorferi murA with mutation conferring resistance to fosfomycin [Borreliella burgdorferi B31]
MHSYIVEGGYKIGGQITASGNKNAALPCILAALLTDEEVILENIPNINDVKVVLDILNDI
GADIAREGNTLKIKVLNIVKTEIDSSFTDLIRASILLLGPFVSRFGKIDMALPGGDVIGK
RRLDTHFYGLCKLGAKLSTKDRRIVLKANKLVGAEMFLDEASVTATENIIMAAVLAEGNT
VIMNAACEPHVQDLCNMLNSMGANILGIGSNVLEIKGVKKLSGTVFRIGADFMQVGSLIS
LAALTGGELEIKKADPQHFRLIRHVYSRLGINFEYDRENVYVRNKQELKVKLDFGGHIPK
IDDGPWPAFPTDLMSIIVVTATQVEGTVLVFEKMFESRMFFVDKLIKMGARIVLCDPHRV
VVTGKSSLKGNVLSSPDVRAGMSLLIAAFVAEGRSEIQNVYQIERGYEDVVNKLINLGAK
IKKVKSQ



>gb|AE000783.1|+|490644-491927|Borreliella burgdorferi murA with mutation conferring resistance to fosfomycin [Borreliella burgdorferi B31]
ATGCATAGTTATATTGTAGAAGGCGGCTATAAGATAGGTGGTCAAATTACAGCTAGTGGGAATAAGAACGCTGCTTTACCCTGTATTTTG
GCTGCTTTACTTACCGATGAAGAGGTTATTTTAGAAAATATTCCTAATATTAATGATGTAAAAGTTGTTTTAGATATTTTAAATGACATA
GGAGCAGATATTGCAAGAGAGGGAAATACTTTAAAAATAAAAGTTTTAAATATTGTGAAAACAGAAATAGATTCTTCTTTTACAGATTTA
ATTAGGGCTTCCATACTTTTATTAGGGCCTTTTGTTTCTAGGTTTGGAAAAATAGATATGGCGCTTCCAGGAGGAGATGTGATTGGAAAG
AGGAGGCTTGATACTCATTTTTACGGGCTTTGCAAGCTGGGGGCCAAGTTAAGCACAAAAGATAGAAGGATTGTTTTAAAGGCTAACAAG
CTTGTTGGCGCTGAAATGTTTTTAGATGAAGCTTCTGTTACAGCCACAGAAAATATCATTATGGCTGCAGTTCTTGCTGAAGGAAATACT
GTTATTATGAACGCTGCTTGTGAGCCACATGTTCAAGATTTGTGTAATATGTTAAATTCAATGGGCGCTAATATTTTAGGAATTGGTTCA
AATGTTTTAGAAATAAAAGGTGTAAAAAAATTAAGTGGGACCGTATTTAGAATAGGAGCCGATTTCATGCAAGTTGGTTCTTTAATTAGC
CTTGCTGCATTAACAGGGGGTGAGTTGGAAATTAAAAAAGCAGATCCCCAACATTTCAGATTAATTAGGCATGTATATTCAAGACTTGGC
ATTAATTTTGAATATGACAGGGAAAATGTATATGTAAGAAATAAACAAGAATTAAAAGTTAAGTTAGATTTTGGTGGGCACATTCCAAAA
ATTGATGATGGCCCATGGCCAGCCTTTCCAACAGACCTTATGAGTATTATTGTAGTTACTGCAACGCAAGTAGAAGGCACAGTTTTAGTT
TTTGAGAAGATGTTTGAATCTAGGATGTTTTTTGTAGATAAATTAATAAAAATGGGTGCTCGAATTGTGCTTTGTGATCCACACCGCGTA
GTAGTTACGGGCAAATCTTCTCTTAAAGGCAATGTTTTGTCTTCTCCGGATGTACGAGCGGGAATGTCTCTTCTTATTGCTGCTTTTGTT
GCTGAAGGTCGCAGCGAGATTCAAAATGTTTATCAAATTGAAAGAGGATACGAAGATGTAGTTAACAAATTGATTAATTTGGGTGCAAAA
ATCAAGAAAGTTAAAAGTCAATAG