| Accession | ARO:3003784 |
| Synonym(s) | Rv1315 |
| CARD Short Name | Mtub_murA_FOF |
| Definition | Mycobacterium tuberculosis murA confers intrinsic resistance to fosfomycin. The presence of an aspartic acid residue in place of the critical cysteine at position 117 that enables fosfomycin binding is believed to be responsible for this intrinsic resistance. |
| AMR Gene Family | antibiotic-resistant murA transferase |
| Drug Class | phosphonic acid antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Resistomes with Sequence Variants | Mycobacterium aviumg+wgs, Mycobacterium colombienseg+wgs, Mycobacterium intracellulareg+wgs, Mycobacterium kansasiig+wgs, Mycobacterium lepraeg+wgs, Mycobacterium lepromatosisg+wgs, Mycobacterium marinumg+wgs, Mycobacterium tuberculosisg+wgs, Mycobacterium ulceransg+wgs, Mycolicibacterium fortuitumg+wgs, Mycolicibacterium septicumwgs |
| Classification | 8 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + mutation conferring antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + phosphonic acid antibiotic [Drug Class] + antibiotic resistant gene variant or mutant |
| Parent Term(s) | 2 ontology terms | Show + confers_resistance_to_antibiotic fosfomycin [Antibiotic] + antibiotic-resistant murA transferase [AMR Gene Family] |
| Publications | De Smet KA, et al. 1999. Microbiology (Reading, Engl.) 145 ( Pt 11):3177-84 Alteration of a single amino acid residue reverses fosfomycin resistance of recombinant MurA from Mycobacterium tuberculosis. (PMID 10589726) Isaza JP, et al. 2012. FEMS Microbiol. Lett. 330(2):113-20 Whole genome shotgun sequencing of one Colombian clinical isolate of Mycobacterium tuberculosis reveals DosR regulon gene deletions. (PMID 22404577) |
Prevalence of Mycobacterium tuberculosis intrinsic murA conferring resistance to fosfomycin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| Mycobacterium avium | 100% | 0% | 98.09% | 0% | 0% |
| Mycobacterium colombiense | 100% | 0% | 100% | 0% | 0% |
| Mycobacterium intracellulare | 100% | 0% | 100% | 0% | 0% |
| Mycobacterium kansasii | 100% | 0% | 100% | 0% | 0% |
| Mycobacterium leprae | 100% | 0% | 100% | 0% | 0% |
| Mycobacterium lepromatosis | 100% | 0% | 100% | 0% | 0% |
| Mycobacterium marinum | 100% | 0% | 98.11% | 0% | 0% |
| Mycobacterium tuberculosis | 99.8% | 0% | 98.82% | 0% | 0% |
| Mycobacterium ulcerans | 100% | 0% | 100% | 0% | 0% |
| Mycolicibacterium fortuitum | 50% | 0% | 82.76% | 0% | 0% |
| Mycolicibacterium septicum | 0% | 0% | 100% | 0% | 0% |
Model Type: protein variant model
Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 750
PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).| Mutation | Mutation type | PubMed | ReSeqTB | CRyPTIC | WHO |
|---|---|---|---|---|---|
| D117C | single resistance variant | PMID:10589726 | no data | no data | no data |
| Curator | Description | Most Recent Edit |
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