| Accession | ARO:3003890 |
| Definition | Mutations to the active importer UhpT, which is involved with the uptake of many phosphorylated sugars, confer resistance to fosfomycin by reducing import of the drug into the bacteria. |
| AMR Gene Family | UhpT |
| Drug Class | fosfomycin |
| Resistance Mechanism | antibiotic target alteration |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + mechanism of antibiotic resistance + antibiotic without defined classification + antibiotic target alteration [Resistance Mechanism] + determinant of antibiotic resistance + fosfomycin [Drug Class] + determinant of fosfomycin resistance + mutation conferring antibiotic resistance |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic fosfomycin [Drug Class] + antibiotic resistant gene variant or mutant + UhpT [AMR Gene Family] |
| Publications | Takahata S, et al. 2010. Int J Antimicrob Agents 35(4): 333-337. Molecular mechanisms of fosfomycin resistance in clinical isolates of Escherichia coli. (PMID 20071153) |
Prevalence of Escherichia coli UhpT with mutation conferring resistance to fosfomycin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS |
|---|---|---|---|
| Acinetobacter baumannii | 0% | 0% | 0% |
| Citrobacter amalonaticus | 66.67% | 0% | 100% |
| Citrobacter freundii | 100% | 0% | 98.45% |
| Citrobacter koseri | 100% | 0% | 95.83% |
| Citrobacter youngae | 50% | 0% | 75% |
| Enterobacter asburiae | 40% | 0% | 53.12% |
| Enterobacter cloacae | 86.67% | 1.3% | 90.07% |
| Enterobacter hormaechei | 97.3% | 0% | 99.37% |
| Enterobacter kobei | 100% | 0% | 95.31% |
| Escherichia coli | 1.27% | 0% | 12.63% |
| Klebsiella oxytoca | 100% | 0% | 96.26% |
| Klebsiella pneumoniae | 0% | 0% | 0% |
| Proteus mirabilis | 0% | 0% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0% |
| Raoultella planticola | 100% | 0% | 100% |
| Salmonella enterica | 98.06% | 0% | 96.14% |
| Shigella dysenteriae | 11.11% | 0% | 20% |
| Shigella sonnei | 0% | 0% | 0.24% |
| Vibrio parahaemolyticus | 0% | 0% | 0.17% |
Model Type: protein variant model
Model Definition: The protein variant model is an AMR detection model. Protein variant models are similar to protein homolog models - they detect the presence of a protein sequence based on its similarity to a curated reference sequence, but secondarily search submitted query sequences for curated sets of mutations shown clinically to confer resistance relative to wild-type. This model includes a protein reference sequence, a curated BLASTP cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of: single resistance variants, insertions, deletions, co-dependent resistance variants, nonsense SNPs, and/or frameshift mutations. Protein variant model matches to reference sequences are categorized on two criteria: strict and loose. A strict match has a BLASTP bitscore above the curated BLASTP cutoff value and contains at least one detected mutation from amongst the mapped resistance variants; a loose match has a BLASTP bitscore below the curated BLASTP cutoff value but still contains at least one detected mutation from amongst the mapped resistance variants. Regardless of BLASTP bitscore, if a sequence does not contain one of the mapped resistance variants, it is not considered a match and not detected by the protein variant model.
Legend:
Bit-score Cut-off (blastP): 850
| PMID: 20071153 | E350Q |