Mycobacterium tuberculosis ddlA mutations confer resistance to cycloserine

Accession ARO:3004941
CARD Short NameMtub_ddlA_DCS
DefinitionPoint mutations that occur within Mycobacterium tuberculosis ddlA gene resulting in resistance to cycloserine.
AMR Gene Familycycloserine resistant ddlA
Drug Classcycloserine-like antibiotic
Resistance Mechanismantibiotic target alteration
Classification9 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic cycloserine [Antibiotic]
+ cycloserine resistant ddlA [AMR Gene Family]
Publications

Li C, et al. 2017. Zhonghua Liu Xing Bing Xue Za Zhi 38(2):240-243 [A preliminary study on the molecular characteristics of D-cycloserine resistance of Mycobacterium tuberculosis]. (PMID 28231674)

Ezewudo M, et al. 2018. Sci Rep 8(1):15382 Integrating standardized whole genome sequence analysis with a global Mycobacterium tuberculosis antibiotic resistance knowledgebase. (PMID 30337678)

Resistomes

Prevalence of Mycobacterium tuberculosis ddlA mutations confer resistance to cycloserine among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GIGRDI-AMR2
No prevalence data


Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 675

PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).

MutationMutation typePubMedReSeqTBCRyPTICWHO
T365Asingle resistance variantno dataReSeqTB-Minimalno datano data

>gb|NP_217497.1|-|Mycobacterium tuberculosis ddlA mutations confer resistance to cycloserine [Mycobacterium tuberculosis H37Rv]
MSANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTD
ANPDALTITNRELPQVKSGSGTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGP
YGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLAADGLPVGAYAVLRPPRSTLH
RQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELEC
GVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQ
LAIRAFAAIDCRGLARVDFFLTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLAT
MIETTLARGVGLH



>gb|NC_000962.3|-|3336796-3337917|Mycobacterium tuberculosis ddlA mutations confer resistance to cycloserine [Mycobacterium tuberculosis H37Rv]
GTGAGTGCTAACGACCGGCGTGATCGGCGTGTCCGCGTTGCCGTCGTGTTCGGCGGGCGCAGCAACGAGCACGCCATCTCGTGTGTGTCC
GCCGGCAGCATCCTGCGCAACCTGGACTCGCGGCGGTTCGACGTGATCGCGGTGGGTATCACCCCGGCAGGTTCGTGGGTGCTCACCGAC
GCCAACCCCGACGCCCTGACGATCACCAACCGGGAGCTTCCTCAGGTCAAATCAGGATCGGGCACCGAGCTGGCGCTGCCGGCCGATCCG
CGGCGTGGTGGCCAGTTGGTGTCGCTGCCGCCCGGGGCCGGCGAGGTTCTGGAGTCGGTCGACGTGGTGTTCCCGGTACTGCACGGCCCG
TACGGCGAGGACGGCACGATCCAGGGACTGCTCGAACTCGCCGGGGTGCCCTACGTGGGCGCCGGTGTGCTGGCCAGTGCCGTCGGCATG
GACAAGGAGTTCACCAAGAAGCTGCTCGCCGCCGATGGACTTCCGGTGGGTGCGTACGCGGTGCTGCGTCCGCCGCGGTCGACACTGCAC
CGCCAGGAGTGCGAACGGCTGGGCTTACCGGTGTTCGTCAAACCCGCCCGAGGCGGCTCGTCGATCGGTGTTAGCCGGGTGTCGAGTTGG
GATCAACTGCCCGCCGCGGTCGCGCGGGCCCGCCGGCATGACCCTAAGGTCATCGTCGAGGCCGCGATCAGCGGCCGCGAGCTGGAATGC
GGTGTGCTCGAAATGCCGGACGGCACACTGGAAGCCAGCACGCTGGGGGAGATCCGGGTGGCCGGGGTGCGGGGACGCGAGGACTCTTTC
TACGACTTCGCAACCAAGTATCTCGACGACGCAGCCGAATTGGACGTGCCCGCCAAGGTCGATGACCAGGTCGCAGAGGCGATTCGTCAG
CTGGCGATCCGGGCGTTCGCGGCTATCGACTGCCGGGGTCTGGCCAGGGTGGACTTCTTCCTCACCGACGACGGTCCGGTGATCAACGAG
ATCAACACGATGCCGGGATTCACCACGATCTCGATGTACCCGCGGATGTGGGCGGCCAGCGGTGTCGACTATCCGACCCTGCTGGCGACG
ATGATCGAGACGACATTGGCCCGCGGCGTGGGCCTGCACTAG

Curator Acknowledgements
Curator Description Most Recent Edit