A bioinformatic database of resistance genes, their products and associated phenotypes.
8582 Ontology Terms, 6442 Reference Sequences, 4480 SNPs, 3354 Publications, 6480 AMR Detection Models
Resistome predictions: 414 pathogens, 24291 chromosomes, 2662 genomic islands, 48212 plasmids, 172216 WGS assemblies, 279120 alleles
YouTube: Canadian Bioinformatics Workshops 2024: Antimicrobial Resistant Gene (AMR) Analysis
The CARD is a rigorously curated collection of characterized, peer-reviewed resistance determinants and associated antibiotics, organized by the Antibiotic Resistance Ontology (ARO) and AMR gene detection models.
The CARD includes tools for analysis of molecular sequences, including BLAST and the Resistance Gene Identifier (RGI) software for prediction of resistome based on homology and SNP models.
CARD data and ontologies can be downloaded in a number of formats, including lists of mutations and molecules with corresponding metadata and citations. RGI software is available as a command-line tool. CARD Bait Capture Platform sequences and protocols available for download. Extensive notes on updates provided.
Computer-generated resistome predictions for 414 important pathogens. Includes sequence variants beyond those reported in the scientific literature, as well as prevalence statistics for AMR genes among pathogens, genomes, and plasmids.
A searchable copy of FungAMR: A comprehensive portrait of antimicrobial resistance mutations in fungi resistance gene and mutation data. Integration to the CARD and the Antibiotic Resistance Ontology (ARO) is in progress. Full details: Bédard et al. bioRxiv 2024.10.07.617009.
A searchable list of Mycobacterium tuberculosis mutations conferring AMR, compiled from the ReSeqTB, CRyPTIC, and World Health Organization catalogs. These data have also been incorporated into CARD, the Antibiotic Resistance Ontology (ARO), and CARD's Resistance Gene Identifier (RGI) software.
The CARD:Live pilot project collects pathogen identification, MLST, AMR gene list, date, and geographical region for genome sequences submitted to RGI online, providing a dynamic view of antibiotic resistant isolates being analyzed around the world.
A robust, reliable, and frequently updated targeted bait capture method for metagenomic detection of antibiotic resistance determinants in complex samples. Synthesis and enrichment protocols, plus bait sequences are available for download. Full details: Hackenberger et al. Appl. Environ. Microbiol. 2025 Feb 28:e0187624.
The Antibiotic Resistance Platform is a cell-based array of mechanistically distinct individual resistance elements in an identical genetic background, spanning 18 classes of antibiotics and over 100 antibiotic resistance genes (ARGs), but researchers can add their own ARGs for direct antibiotic susceptibility testing. Full details are available at the ARP website.