The Comprehensive Antibiotic Resistance Database

A bioinformatic database of resistance genes, their products and associated phenotypes.

7170 Ontology Terms, 5194 Reference Sequences, 2008 SNPs, 3279 Publications, 5242 AMR Detection Models

Resistome predictions: 413 pathogens, 24291 chromosomes, 2662 genomic islands, 48212 plasmids, 172216 WGS assemblies, 276270 alleles

YouTube: Canadian Bioinformatics Workshops 2023: Antimicrobial Resistant Gene (AMR) Analysis


The CARD is a rigorously curated collection of characterized, peer-reviewed resistance determinants and associated antibiotics, organized by the Antibiotic Resistance Ontology (ARO) and AMR gene detection models.


The CARD includes tools for analysis of molecular sequences, including BLAST and the Resistance Gene Identifier (RGI) software for prediction of resistome based on homology and SNP models.


CARD data and ontologies can be downloaded in a number of formats. RGI software is available as a command-line tool. CARD Bait Capture Platform sequences and protocols available for download. Extensive notes on updates provided.

Resistomes, Variants, & Prevalence

Computer-generated resistome predictions for 413 important pathogens. Includes sequence variants beyond those reported in the scientific literature, as well as prevalence statistics for AMR genes among pathogens, genomes, and plasmids.


The CARD:Live project collects pathogen identification, MLST, AMR gene list, date, and geographical region for genome sequences submitted to RGI online, providing a dynamic view of antibiotic resistant isolates being analyzed around the world.

CARD Bait Capture Platform

UPDATED March 2024: A robust and reliable targeted bait capture method for metagenomic detection of antibiotic resistance determinants in complex samples, including hybridization bait FASTA sequences and laboratory protocols, is available for download. Hackenberger et al. bioRxiv 2024.03.27.587061.