| Accession | ARO:3000074 |
| CARD Short Name | emrB |
| Definition | emrB is a translocase in the emrB -TolC efflux protein in E. coli. It recognizes substrates including carbonyl cyanide m-chlorophenylhydrazone (CCCP), nalidixic acid, and thioloactomycin. |
| AMR Gene Family | major facilitator superfamily (MFS) antibiotic efflux pump |
| Drug Class | fluoroquinolone antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Perfect Matches | Escherichia colig+p+wgs, Escherichia fergusoniig+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Resistomes with Sequence Variants | Citrobacter amalonaticusg+wgs, Citrobacter freundiig+wgs, Citrobacter koserig+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniig+wgs, Citrobacter youngaeg+wgs, Cronobacter dublinensisg+wgs, Cronobacter malonaticusg+wgs, Cronobacter sakazakiig+wgs, Cronobacter turicensiswgs, Cronobacter universalisg+wgs, Enterobacter asburiaeg+wgs, Enterobacter cancerogenusg+wgs, Enterobacter chengduensisg+wgs, Enterobacter cloacaeg+p+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeig+wgs, Enterobacter roggenkampiig+wgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs, Escherichia fergusoniig+wgs, Escherichia marmotaeg+wgs, Leclercia adecarboxylatag+wgs, Salmonella bongorig+wgs, Salmonella entericag+p+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + antibiotic molecule + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + fluoroquinolone antibiotic [Drug Class] + major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family] + nalidixic acid [Antibiotic] |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Lomovskaya O, et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89(19):8938-42 Emr, an Escherichia coli locus for multidrug resistance. (PMID 1409590) |
Prevalence of emrB among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Citrobacter amalonaticus | 100% | 0% | 89.09% | 0% |
| Citrobacter freundii | 77.87% | 0% | 50.29% | 0% |
| Citrobacter koseri | 100% | 0% | 49.55% | 0% |
| Citrobacter portucalensis | 100% | 0% | 62.16% | 0% |
| Citrobacter werkmanii | 71.43% | 0% | 61.54% | 0% |
| Citrobacter youngae | 100% | 0% | 100% | 0% |
| Cronobacter dublinensis | 66.67% | 0% | 84.62% | 0% |
| Cronobacter malonaticus | 100% | 0% | 85.45% | 0% |
| Cronobacter sakazakii | 100% | 0% | 88.57% | 0% |
| Cronobacter turicensis | 0% | 0% | 83.33% | 0% |
| Cronobacter universalis | 100% | 0% | 100% | 0% |
| Enterobacter asburiae | 100% | 0% | 70.36% | 0% |
| Enterobacter cancerogenus | 100% | 0% | 100% | 0% |
| Enterobacter chengduensis | 100% | 0% | 84% | 0% |
| Enterobacter cloacae | 98.21% | 0.56% | 71.88% | 0% |
| Enterobacter hormaechei | 99.28% | 0.06% | 66.59% | 0% |
| Enterobacter kobei | 100% | 0% | 67.69% | 0% |
| Enterobacter roggenkampii | 97.67% | 0% | 61.51% | 0% |
| Escherichia albertii | 88.57% | 0% | 60.65% | 0% |
| Escherichia coli | 67.48% | 0.01% | 61.21% | 0% |
| Escherichia fergusonii | 100% | 0% | 51.09% | 0% |
| Escherichia marmotae | 100% | 0% | 66.67% | 0% |
| Leclercia adecarboxylata | 100% | 0% | 60.47% | 0% |
| Salmonella bongori | 100% | 0% | 94.74% | 0% |
| Salmonella enterica | 94.38% | 0.05% | 80.51% | 0% |
| Shigella boydii | 93.33% | 0% | 92.22% | 0% |
| Shigella dysenteriae | 28.57% | 0% | 70% | 0% |
| Shigella flexneri | 98% | 0% | 78.57% | 0% |
| Shigella sonnei | 100% | 0% | 94.81% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 900