| Accession | ARO:3000074 |
| CARD Short Name | emrB |
| Definition | emrB is a translocase in the emrB -TolC efflux protein in E. coli. It recognizes substrates including carbonyl cyanide m-chlorophenylhydrazone (CCCP), nalidixic acid, and thioloactomycin. |
| AMR Gene Family | major facilitator superfamily (MFS) antibiotic efflux pump |
| Drug Class | fluoroquinolone antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Perfect Matches | Escherichia colig+p+wgs, Escherichia fergusoniig+wgs, Shigella boydiig+wgs, Shigella dysenteriaewgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Resistomes with Sequence Variants | Acinetobacter baumanniiwgs, Burkholderia cepaciawgs, Citrobacter amalonaticusg+wgs, Citrobacter freundiig+wgs, Citrobacter koserig+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniig+wgs, Citrobacter youngaeg+wgs, Cronobacter sakazakiig+wgs, Enterobacter asburiaeg+wgs, Enterobacter chengduensisg+wgs, Enterobacter cloacaeg+p+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeig+wgs, Enterobacter roggenkampiig+wgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs, Escherichia fergusoniig+wgs, Escherichia marmotaeg+wgs, Klebsiella pneumoniaeg+wgs, Klebsiella quasipneumoniaewgs, Leclercia adecarboxylatag+wgs, Listeria monocytogeneswgs, Mycobacterium aviumwgs, Pseudomonas aeruginosawgs, Salmonella entericag+p+wgs, Serratia marcescenswgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs, Streptococcus suiswgs, Vibrio parahaemolyticuswgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic efflux [Resistance Mechanism] + fluoroquinolone antibiotic [Drug Class] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + nalidixic acid [Antibiotic] + major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family] |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Lomovskaya O, et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89(19):8938-42 Emr, an Escherichia coli locus for multidrug resistance. (PMID 1409590) |
Prevalence of emrB among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 263 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 0% | 0% | 0.06% | 0% |
| Burkholderia cepacia | 0% | 0% | 0.45% | 0% |
| Citrobacter amalonaticus | 100% | 0% | 87.5% | 0% |
| Citrobacter freundii | 81.54% | 0% | 60.25% | 0% |
| Citrobacter koseri | 100% | 0% | 46.67% | 0% |
| Citrobacter portucalensis | 100% | 0% | 86% | 0% |
| Citrobacter werkmanii | 71.43% | 0% | 100% | 0% |
| Citrobacter youngae | 100% | 0% | 100% | 0% |
| Cronobacter sakazakii | 100% | 0% | 93.13% | 0% |
| Enterobacter asburiae | 100% | 0% | 87.5% | 0% |
| Enterobacter chengduensis | 100% | 0% | 90.91% | 0% |
| Enterobacter cloacae | 97.73% | 0.74% | 80% | 0% |
| Enterobacter hormaechei | 98.81% | 0.17% | 80.12% | 0% |
| Enterobacter kobei | 91.67% | 0% | 86.07% | 0% |
| Enterobacter roggenkampii | 96.15% | 0% | 73.94% | 0% |
| Escherichia albertii | 90% | 0% | 94.25% | 0% |
| Escherichia coli | 61.15% | 0.02% | 74.49% | 0% |
| Escherichia fergusonii | 100% | 0% | 44.62% | 0% |
| Escherichia marmotae | 100% | 0% | 64.29% | 0% |
| Klebsiella pneumoniae | 0.09% | 0% | 0.29% | 0% |
| Klebsiella quasipneumoniae | 0% | 0% | 0.36% | 0% |
| Leclercia adecarboxylata | 100% | 0% | 90.48% | 0% |
| Listeria monocytogenes | 0% | 0% | 0.03% | 0% |
| Mycobacterium avium | 0% | 0% | 0.48% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0.05% | 0% |
| Salmonella enterica | 94.22% | 0.07% | 89.18% | 0% |
| Serratia marcescens | 0% | 0% | 0.15% | 0% |
| Shigella boydii | 100% | 0% | 95.7% | 0% |
| Shigella dysenteriae | 90.91% | 0% | 96.43% | 0% |
| Shigella flexneri | 100% | 0% | 83.5% | 0% |
| Shigella sonnei | 100% | 0% | 95.93% | 0% |
| Streptococcus suis | 0% | 0% | 0.06% | 0% |
| Vibrio parahaemolyticus | 0% | 0% | 0.06% | 0% |
Model Type: protein homolog model
Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.
Bit-score Cut-off (blastP): 900