Staphylococcus aureus ileS with mutation conferring resistance to mupirocin

Accession ARO:3003729
CARD Short NameSaur_ileS_MUP
DefinitionPoint mutations to the isoleucyl-tRNA synthetase (ileS) in Staphylococcus aureus that confer resistance to mupirocin.
AMR Gene Familyantibiotic-resistant isoleucyl-tRNA synthetase (ileS)
Drug Classmupirocin-like antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Sequence VariantsStaphylococcus aureusg+wgs
Classification8 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ antibiotic-resistant isoleucyl-tRNA synthetase (ileS) [AMR Gene Family]
+ confers_resistance_to_antibiotic mupirocin [Antibiotic]
Publications

Antonio M, et al. 2002. Antimicrob. Agents Chemother. 46(2):438-42 Mutations affecting the Rossman fold of isoleucyl-tRNA synthetase are correlated with low-level mupirocin resistance in Staphylococcus aureus. (PMID 11796355)

Resistomes

Prevalence of Staphylococcus aureus ileS with mutation conferring resistance to mupirocin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Staphylococcus aureus0.79%0%1.82%0%
Show Perfect Only


Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 1800

Type of Antibiotic Resistance: Intrinsic or chromosomally-encoded

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:

PMID: 11796355V588F V631F

>gb|CAA52296.1|+|Staphylococcus aureus ileS with mutation conferring resistance to mupirocin [Staphylococcus aureus]
MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKNKGNETFILHDGPPYAN
GNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIEQALTKKGVDRKKMSTAEF
REKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYEAAQIRIFGEMADKGLIYKGK
KPVYWSPSSESSLAEAEIEYHDKRSASIYVAFNVKDDKGVVDADAKFIIWTTTPWTIPSN
VAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALDWDKASIKLEKEYTGKELEYVVAQH
PFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDDYIVGQKYELPVISPIDDKGVFTEEGG
QFEGMFYDKANKAVTDLLTEKGALLKLDFITHSYPHDWRTKKPVIFRATPQWFASISKVR
QDILDAIENTNFKVNWGKTRIYNMVRDRGEWVISRQRVWGVPLPVFYAENGEIIMTKETV
NHVADLFAEHGSNIWFEREAKDLLPEGFTHPGSPNGTFTKETDIMDVWFDSGSSHRGVLE
TRPELSFPADMYLEGSDQYRGWFNSSITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSL
GNVIVPDQVVKQKGADIARLWVSSTDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNIN
DFNPDTDSIPESELLEVDRYLLNRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFY
LDYGKDILYIEQRDSHIRRSMQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVH
LADMPKVVEVDQALLDKWRTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNAS
EFLTSFDALHQLFIVSQVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVD
ELTHLCPRCQQVVKSLV



>gb|X74219.1|+|91-2844|Staphylococcus aureus ileS with mutation conferring resistance to mupirocin [Staphylococcus aureus]
ATGGATTACAAAGAAACGTTATTAATGCCTAAAACAGATTTCCCAATGCGAGGTGGTTTACCAAACAAGGAACCGCAAATTCAAGAAAAA
TGGGATGCAGAAGATCAATACCATAAAGCGTTAGAAAAAAATAAAGGTAACGAAACATTCATTTTACATGATGGCCCACCATACGCGAAT
GGTAACTTACATATGGGACATGCCTTGAACAAAATTTTAAAAGACTTTATTGTACGTTATAAAACTATGCAAGGGTTCTATGCACCATAC
GTACCAGGTTGGGATACACATGGTTTGCCAATTGAACAAGCATTAACGAAAAAAGGTGTTGACCGTAAGAAAATGTCAACAGCTGAATTC
CGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGAT
CCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAA
AAGCCAGTTTATTGGTCTCCTTCAAGTGAGTCTTCATTAGCAGAAGCAGAAATTGAATATCACGATAAACGTTCAGCATCAATTTACGTT
GCATTTAACGTTAAAGATGACAAAGGTGTCGTTGATGCAGATGCTAAATTTATTATCTGGACAACAACGCCATGGACAATTCCATCAAAT
GTTGCGATTACCGTTCATCCAGAATTAAAATACGGTCAATACAATGTAAATGGCGAAAAATATATTATTGCAGAAGCCTTATCTGACGCC
GTAGCAGAAGCACTGGATTGGGATAAAGCATCAATCAAATTAGAAAAAGAATACACAGGTAAGGAATTGGAGTATGTTGTAGCACAACAT
CCATTCTTAGATAGAGAATCGTTAGTGATTAATGGTGATCATGTTACTACAGATGCTGGTACAGGTTGTGTACATACAGCACCAGGTCAC
GGGGAAGATGACTATATTGTTGGTCAAAAATATGAATTGCCAGTAATTAGTCCAATCGATGATAAAGGTGTATTTACTGAAGAAGGCGGC
CAATTTGAAGGAATGTTCTATGATAAAGCTAATAAAGCCGTTACTGATTTATTAACAGAAAAAGGTGCACTATTAAAATTAGACTTTATT
ACACATAGCTATCCACACGACTGGAGAACAAAAAAACCTGTAATTTTCCGTGCTACACCACAATGGTTTGCTTCAATCAGTAAAGTAAGA
CAAGATATTTTAGATGCAATCGAAAATACAAACTTCAAAGTAAATTGGGGTAAAACACGTATTTACAATATGGTTCGTGACCGTGGCGAA
TGGGTTATTTCTCGTCAACGTGTTTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTG
AATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACAT
CCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATGGACGTTTGGTTTGATTCTGGTTCATCACACCGTGGCGTGTTGGAA
ACAAGACCGGAATTAAGTTTCCCAGCAGATATGTATTTAGAAGGTAGTGACCAATATCGTGGTTGGTTCAACTCTTCTATTACAACTTCA
GTTGCTACAAGAGGAGTATCACCTTATAAATTCTTACTTTCTCATGGTTTTGTTATGGACGGTGAAGGTAAGAAAATGAGTAAATCTTTA
GGTAATGTGATTGTACCTGACCAAGTGGTTAAACAAAAAGGTGCTGATATTGCGAGACTTTGGGTAAGTAGTACGGACTATTTAGCTGAT
GTTAGAATTTCTGATGAAATTTTAAAACAAACATCTGATGTTTATCGTAAAATCAGAAATACATTAAGATTTATGTTAGGTAATATTAAT
GATTTCAATCCTGATACAGATAGCATTCCTGAATCAGAGTTATTAGAAGTTGATCGTTACTTGCTAAATCGTTTACGTGAATTTACTGCA
AGTACGATTAACAACTATGAAAACTTTGACTACTTAAATATTTATCAAGAAGTTCAAAACTTTATCAATGTTGAGTTAAGTAATTTCTAT
TTGGATTACGGTAAAGATATTTTATATATTGAACAACGTGATTCTCATATCCGTCGTAGTATGCAAACAGTGTTATATCAAATTTTAGTT
GATATGACGAAGTTGTTAGCACCAATCTTAGTGCATACAGCTGAAGAAGTTTGGTCTCATACACCACATGTTAAAGAAGAAAGTGTTCAC
TTAGCAGACATGCCTAAAGTTGTAGAAGTAGATCAAGCTTTATTGGATAAATGGCGTACATTTATGAATTTACGTGATGATGTGAACCGT
GCATTAGAAACTGCTCGTAATGAAAAAGTTATTGGTAAATCATTAGAAGCTAAAGTTACGATTGCTAGTAACGATAAATTTAATGCATCT
GAATTCTTAACTTCATTTGATGCATTACATCAATTATTTATCGTGTCACAAGTTAAAGTTGTAGATAAGTTAGATGATCAGGCAACAGCT
TATGAACATGGTGATATTGTCATCGAACATGCAGATGGTGAAAAATGTGAAAGATGTTGGAACTATTCAGAGGATCTTGGTGCTGTTGAT
GAATTGACGCATCTATGCCCACGATGCCAACAAGTTGTAAAATCACTTGTATAA