Enterococcus faecalis drmA with mutation conferring daptomycin resistance

Accession ARO:3003813
CARD Short NameEfae_drmA_DAP
DefinitiondrmA is an uncharacterized 6-pass membrane protein, with mutations to the protein causing modest resistance to daptomycin.
AMR Gene Familydaptomycin resistant drmA
Drug Classpeptide antibiotic
Resistance Mechanismantibiotic target alteration
Classification10 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ daptomycin resistant drmA [AMR Gene Family]
+ confers_resistance_to_antibiotic daptomycin [Antibiotic]
Publications

Miller C, et al. 2013. Antimicrob Agents Chemother 57(11): 5373-5383. Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance. (PMID 23959318)

Resistomes

Prevalence of Enterococcus faecalis drmA with mutation conferring daptomycin resistance among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
No prevalence data


Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 400

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:

PMID: 23959318N150fs L4STOP

>gb|AAO81566.1|-|Enterococcus faecalis drmA with mutation conferring daptomycin resistance [Enterococcus faecalis V583]
MQFLKKYGGYFLVLGVLLDFFTPYYVGFKDQGYNQLTEVISLLGDVNSPVRENFNRLTII
AGMLMLASLPRIYAIFSRKTKKGAWLVVAMIGAYGLFDCIFSGLFSVDTSSAGTVAAALH
NGGSAVGYTGFLLLSGVLTIIYSKYGSQKNKNLFGFLFILCMLAAGLYGLARIPQLQQVK
PFNYLGLWQRVSSFCNYLPMLALCLQTKTNDKFD



>gb|AE016830.1|-|1738705-1739349|Enterococcus faecalis drmA with mutation conferring daptomycin resistance [Enterococcus faecalis V583]
ATGCAGTTTTTAAAAAAATATGGCGGCTATTTTTTAGTTTTAGGGGTCTTACTTGATTTTTTTACACCTTATTATGTCGGATTCAAAGAT
CAAGGTTACAATCAATTGACAGAAGTAATTAGCTTATTAGGTGATGTGAATAGTCCAGTTAGGGAAAACTTTAATCGGTTAACTATCATT
GCGGGAATGTTGATGTTAGCAAGTTTACCTAGGATATATGCTATTTTTTCCAGAAAAACGAAAAAAGGTGCTTGGTTAGTAGTGGCAATG
ATTGGCGCTTATGGTTTATTTGATTGTATTTTCAGTGGGCTCTTCAGCGTAGACACTTCAAGCGCTGGCACAGTTGCTGCCGCGCTTCAT
AACGGAGGATCAGCAGTCGGTTATACAGGCTTTTTGTTGTTATCAGGCGTCTTAACCATTATCTACAGTAAATACGGTTCGCAAAAAAAT
AAAAACCTTTTTGGCTTTTTGTTTATTTTATGTATGCTTGCAGCCGGCTTATATGGCTTAGCAAGGATTCCACAGTTGCAACAGGTAAAA
CCATTTAATTATTTAGGCTTATGGCAAAGAGTGAGTTCATTTTGTAATTATCTACCAATGCTGGCACTGTGTTTACAAACTAAAACCAAT
GACAAATTTGATTGA