Staphylococcus aureus menA with mutation conferring resistance to lysocin

Accession ARO:3003917
DefinitionmenA encodes a 1,4-dihydroxy-2-naphthoate octaprenyltransferase, with mutations to the protein conferring resistance to lysocin E.
AMR Gene Familylysocin resistant menA
Drug Classpeptide antibiotic
Resistance Mechanismantibiotic target alteration
Classification9 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic Lysocin E [Antibiotic]
+ lysocin resistant menA [AMR Gene Family]
Publications

Hamamoto H, et al. 2015. Nat. Chem. Biol. 11(2):127-33 Lysocin E is a new antibiotic that targets menaquinone in the bacterial membrane. (PMID 25485686)

Resistomes

Prevalence of Staphylococcus aureus menA with mutation conferring resistance to lysocin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 88 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
No prevalence data


Detection Models

Model Type: protein variant model

Model Definition: The protein variant model is an AMR detection model. Protein variant models are similar to protein homolog models - they detect the presence of a protein sequence based on its similarity to a curated reference sequence, but secondarily search submitted query sequences for curated sets of mutations shown clinically to confer resistance relative to wild-type. This model includes a protein reference sequence, a curated BLASTP cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of: single resistance variants, insertions, deletions, co-dependent resistance variants, nonsense SNPs, and/or frameshift mutations. Protein variant model matches to reference sequences are categorized on two criteria: strict and loose. A strict match has a BLASTP bitscore above the curated BLASTP cutoff value and contains at least one detected mutation from amongst the mapped resistance variants; a loose match has a BLASTP bitscore below the curated BLASTP cutoff value but still contains at least one detected mutation from amongst the mapped resistance variants. Regardless of BLASTP bitscore, if a sequence does not contain one of the mapped resistance variants, it is not considered a match and not detected by the protein variant model.

Bit-score Cut-off (blastP): 550

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:


>gb|ABD30105.1|-|Staphylococcus aureus menA with mutation conferring resistance to lysocin [Staphylococcus aureus subsp. aureus NCTC 8325]
MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGSEDHIKISLFIAMLLAC
LLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELVLRLAIAFYILAAILGLFL
AANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSGVFMGMFIIVIAFFIQTGNIQ
SYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLPILLGKNASLTFMAIMYFIAYAF
IVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKNDTPPTMMPAMAAAGKTNTFFGLLYA
LGIYISALFAGI



>gb|CP000253.1|-|951802-952740|Staphylococcus aureus menA with mutation conferring resistance to lysocin [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGAGTAATCAATATCAGCAATATTCTACAGTTAAGAAATATTGGCATTTAATGCGTCCTCATACATTAACTGCTTCCGTAGTACCCGTT
TTAGTTGGTACAGCAGCATCTAAAATATATTTTCTTGGTAGCGAAGATCATATTAAAATCAGCCTATTCATTGCCATGTTACTAGCATGC
TTACTTATTCAAGCAGCAACTAATATGTTTAATGAATACTATGATTATAAAAAAGGCCTCGATGATCATGAATCTGTAGGCATTGGTGGT
GCCATTGTTCGCAACGGTATGAGCCCAGAGCTTGTGCTACGATTAGCCATTGCATTTTACATCTTAGCAGCAATATTAGGTTTGTTTTTA
GCTGCTAACTCTTCATTTTGGTTATTACCAGTTGGATTAGTATGTATGGCTGTTGGTTACCTATATACAGGTGGCCCTTTCCCTATTTCA
TGGACGCCTTTCGGTGAATTATTCTCAGGCGTATTTATGGGTATGTTTATTATCGTTATTGCATTCTTTATTCAAACTGGCAATATTCAA
AGTTATGTAATTTGGTTAAGTGTACCTATAGTAATCACTATCGGTTTAATTAATATGGCTAACAATATTCGCGACCGTGTCAAAGATAAA
GCAAGTGGTCGCAAAACTTTACCCATTCTATTAGGTAAAAATGCTTCTTTAACATTTATGGCAATCATGTACTTTATCGCTTATGCCTTT
ATTGTACTTACGATCATTATTAAACCTGGTGGCTCATTATTTTACTTACTTGCGTTGTTATCATTCCCAATGCCTGTTAAAGTTATCAGA
CGTTTCAAGAAGAATGATACACCGCCTACAATGATGCCAGCAATGGCTGCTGCTGGTAAAACAAATACATTTTTCGGTTTATTATATGCA
TTAGGTATTTATATTAGTGCATTATTTGCAGGCATTTAA