| Accession | ARO:3003950 |
| CARD Short Name | msbA |
| Definition | MsbA is a multidrug resistance transporter homolog from E. coli and belongs to a superfamily of transporters that contain an adenosine triphosphate (ATP) binding cassette (ABC) which is also called a nucleotide-binding domain (NBD). MsbA is a member of the MDR-ABC transporter group by sequence homology. MsbA transports lipid A, a major component of the bacterial outer cell membrane, and is the only bacterial ABC transporter that is essential for cell viability. |
| AMR Gene Family | ATP-binding cassette (ABC) antibiotic efflux pump |
| Drug Class | nitroimidazole antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Perfect Matches | Escherichia colig+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Resistomes with Sequence Variants | Citrobacter amalonaticusg+wgs, Citrobacter freundiig+wgs, Citrobacter koserig+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniig+wgs, Citrobacter youngaeg+wgs, Cronobacter condimentig+wgs, Cronobacter dublinensisg+wgs, Cronobacter malonaticusg+wgs, Cronobacter sakazakiig+wgs, Cronobacter turicensiswgs, Cronobacter universalisg+wgs, Enterobacter asburiaeg+wgs, Enterobacter cancerogenusg+wgs, Enterobacter chengduensisg+wgs, Enterobacter cloacaeg+wgs, Enterobacter hormaecheig+wgs, Enterobacter kobeig+wgs, Enterobacter roggenkampiig+wgs, Escherichia albertiig+wgs, Escherichia colig+wgs, Escherichia fergusoniig+wgs, Escherichia marmotaeg+wgs, Klebsiella aerogenesg+wgs, Klebsiella huaxiensisg+wgs, Klebsiella michiganensisg+wgs, Klebsiella oxytocag+wgs, Klebsiella pneumoniaeg+wgs, Klebsiella quasipneumoniaeg+wgs, Kosakonia arachidisg+wgs, Leclercia adecarboxylatag+wgs, Raoultella planticolag+wgs, Salmonella bongorig+wgs, Salmonella entericag+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Classification | 7 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic efflux [Resistance Mechanism] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + nitroimidazole antibiotic [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show + ATP-binding cassette (ABC) antibiotic efflux pump [AMR Gene Family] + confers_resistance_to_antibiotic metronidazole [Antibiotic] |
| Publications | Boyanova L, et al. 2002. J. Med. Microbiol. 51(9):786-90 Prevalence of resistant Helicobacter pylori isolates in Bulgarian children. (PMID 12358070) Li XZ and Nikaido H. 2009. Drugs 69(12): 1555-1623. Efflux-mediated drug resistance in bacteria: an update. (PMID 19678712) Zgurskaya HI, et al. 2002. Int. J. Med. Microbiol. 292(2):95-105 Molecular analysis of efflux pump-based antibiotic resistance. (PMID 12195740) Singh H, et al. 2016. Nat Commun 7:12387 ATP-dependent substrate transport by the ABC transporter MsbA is proton-coupled. (PMID 27499013) |
Prevalence of msbA among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Citrobacter amalonaticus | 100% | 0% | 87.27% | 0% |
| Citrobacter freundii | 100% | 0% | 50.87% | 0% |
| Citrobacter koseri | 100% | 0% | 48.65% | 0% |
| Citrobacter portucalensis | 100% | 0% | 62.16% | 0% |
| Citrobacter werkmanii | 100% | 0% | 61.54% | 0% |
| Citrobacter youngae | 100% | 0% | 100% | 0% |
| Cronobacter condimenti | 100% | 0% | 100% | 0% |
| Cronobacter dublinensis | 100% | 0% | 100% | 0% |
| Cronobacter malonaticus | 100% | 0% | 83.64% | 0% |
| Cronobacter sakazakii | 100% | 0% | 92.38% | 0% |
| Cronobacter turicensis | 0% | 0% | 83.33% | 0% |
| Cronobacter universalis | 100% | 0% | 100% | 0% |
| Enterobacter asburiae | 100% | 0% | 70.75% | 0% |
| Enterobacter cancerogenus | 100% | 0% | 100% | 0% |
| Enterobacter chengduensis | 100% | 0% | 84% | 0% |
| Enterobacter cloacae | 100% | 0% | 73.48% | 0% |
| Enterobacter hormaechei | 99.64% | 0% | 67.07% | 0% |
| Enterobacter kobei | 100% | 0% | 68.56% | 0% |
| Enterobacter roggenkampii | 97.67% | 0% | 62.23% | 0% |
| Escherichia albertii | 100% | 0% | 62.58% | 0% |
| Escherichia coli | 67.84% | 0% | 61.81% | 0% |
| Escherichia fergusonii | 98.36% | 0% | 51.09% | 0% |
| Escherichia marmotae | 100% | 0% | 70.83% | 0% |
| Klebsiella aerogenes | 100% | 0% | 79.1% | 0% |
| Klebsiella huaxiensis | 100% | 0% | 66.67% | 0% |
| Klebsiella michiganensis | 100% | 0% | 68.88% | 0% |
| Klebsiella oxytoca | 100% | 0% | 74.79% | 0% |
| Klebsiella pneumoniae | 99.7% | 0% | 57.31% | 0% |
| Klebsiella quasipneumoniae | 100% | 0% | 73.55% | 0% |
| Kosakonia arachidis | 100% | 0% | 100% | 0% |
| Leclercia adecarboxylata | 100% | 0% | 60.47% | 0% |
| Raoultella planticola | 100% | 0% | 94.87% | 0% |
| Salmonella bongori | 100% | 0% | 94.74% | 0% |
| Salmonella enterica | 95.58% | 0% | 80.88% | 0% |
| Shigella boydii | 93.33% | 0% | 96.67% | 0% |
| Shigella dysenteriae | 100% | 0% | 93.33% | 0% |
| Shigella flexneri | 100% | 0% | 80.43% | 0% |
| Shigella sonnei | 100% | 0% | 95.84% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 1000