Escherichia coli fabI mutations conferring resistance to isoniazid and triclosan

Accession ARO:3004045
CARD Short NameEcol_fabI_MULT
DefinitionfabI is a enoyl-acyl carrier reductase used in lipid metabolism and fatty acid biosynthesis. The bacterial biocide Triclosan blocks the final reduction step in fatty acid elongation, inhibiting biosynthesis. Point mutations in fabI can confer resistance to Triclosan and Isoniazid.
AMR Gene Familyantibiotic resistant fabI
Drug Classdisinfecting agents and antiseptics, isoniazid-like antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Sequence VariantsEnterobacter asburiaewgs, Enterobacter cloacaewgs, Enterobacter hormaecheiwgs, Enterobacter kobeiwgs, Escherichia coliwgs, Klebsiella michiganensiswgs, Klebsiella pneumoniaeg+wgs, Klebsiella quasipneumoniaewgs, Pectobacterium parmentierig+wgs, Providencia stuartiig+wgs
Classification9 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ antibiotic resistant fabI [AMR Gene Family]
+ confers_resistance_to_antibiotic triclosan [Antibiotic]

Khan R, et al. 2016. Sci Rep 6:32322 Triclosan Resistome from Metagenome Reveals Diverse Enoyl Acyl Carrier Protein Reductases and Selective Enrichment of Triclosan Resistance Genes. (PMID 27577999)


Prevalence of Escherichia coli fabI mutations conferring resistance to isoniazid and triclosan among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Enterobacter asburiae0%0%0.79%0%
Enterobacter cloacae0%0%0.32%0%
Enterobacter hormaechei0%0%0.13%0%
Enterobacter kobei0%0%0.44%0%
Escherichia coli0%0%0.03%0%
Klebsiella michiganensis0%0%1.06%0%
Klebsiella pneumoniae0.3%0%0.39%0%
Klebsiella quasipneumoniae0%0%0.13%0%
Pectobacterium parmentieri100%0%94.12%0%
Providencia stuartii6.25%0%2.27%0%
Show Perfect Only

Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 450


  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB

Published Variants:

PMID: 27577999G93V G93S G93A M159T F203V F203A F203C F203L

>gb|AAC74370.1|-|Escherichia coli fabI mutations conferring resistance to isoniazid and triclosan [Escherichia coli str. K-12 substr. MG1655]

>gb|U00096.3|-|1350251-1351039|Escherichia coli fabI mutations conferring resistance to isoniazid and triclosan [Escherichia coli str. K-12 substr. MG1655]