| Accession | ARO:3004045 |
| CARD Short Name | Ecol_fabI_MULT |
| Definition | fabI is a enoyl-acyl carrier reductase used in lipid metabolism and fatty acid biosynthesis. The bacterial biocide Triclosan blocks the final reduction step in fatty acid elongation, inhibiting biosynthesis. Point mutations in fabI can confer resistance to Triclosan and Isoniazid. |
| AMR Gene Family | antibiotic resistant fabI |
| Drug Class | isoniazid-like antibiotic, disinfecting agents and antiseptics |
| Resistance Mechanism | antibiotic target alteration |
| Resistomes with Sequence Variants | Enterobacter asburiaewgs, Enterobacter cloacaewgs, Enterobacter hormaecheiwgs, Enterobacter kobeiwgs, Escherichia coliwgs, Klebsiella michiganensiswgs, Klebsiella pneumoniaeg+wgs, Klebsiella quasipneumoniaewgs, Pectobacterium parmentierig+wgs, Providencia stuartiig+wgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + mutation conferring antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + isoniazid-like antibiotic [Drug Class] + disinfecting agents and antiseptics [Drug Class] + antibiotic resistant gene variant or mutant |
| Parent Term(s) | 2 ontology terms | Show + confers_resistance_to_antibiotic triclosan [Antibiotic] + antibiotic resistant fabI [AMR Gene Family] |
| Publications | Khan R, et al. 2016. Sci Rep 6:32322 Triclosan Resistome from Metagenome Reveals Diverse Enoyl Acyl Carrier Protein Reductases and Selective Enrichment of Triclosan Resistance Genes. (PMID 27577999) |
Prevalence of Escherichia coli fabI mutations conferring resistance to isoniazid and triclosan among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| Enterobacter asburiae | 0% | 0% | 0.79% | 0% | 0% |
| Enterobacter cloacae | 0% | 0% | 0.32% | 0% | 0% |
| Enterobacter hormaechei | 0% | 0% | 0.13% | 0% | 0% |
| Enterobacter kobei | 0% | 0% | 0.44% | 0% | 0% |
| Escherichia coli | 0% | 0% | 0.03% | 0% | 0% |
| Klebsiella michiganensis | 0% | 0% | 4.26% | 0% | 0% |
| Klebsiella pneumoniae | 0.3% | 0% | 0.42% | 0% | 0% |
| Klebsiella quasipneumoniae | 0% | 0% | 0.13% | 0% | 0% |
| Pectobacterium parmentieri | 100% | 0% | 94.12% | 0% | 0% |
| Providencia stuartii | 6.25% | 0% | 2.27% | 0% | 0% |
Model Type: protein variant model
Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 450
PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).| Mutation | Mutation type | PubMed |
|---|---|---|
| G93S | single resistance variant | PMID:27577999 |
| G93V | single resistance variant | PMID:27577999 |
| G93A | single resistance variant | PMID:27577999 |
| M159T | single resistance variant | PMID:27577999 |
| F203L | single resistance variant | PMID:27577999 |
| F203A | single resistance variant | PMID:27577999 |
| F203V | single resistance variant | PMID:27577999 |
| F203C | single resistance variant | PMID:27577999 |
| Curator | Description | Most Recent Edit |
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