rifamycin-resistant beta-subunit of RNA polymerase (rpoB) [AMR Gene Family]

Accession ARO:3000210
DefinitionRifampin resistant RNA polymerases include amino acids substitutions which disrupt the affinity of rifampin for its binding site. These mutations are frequently concentrated in the rif I region of the beta-subunit and most often involve amino acids which make direct interactions with rifampin. However, mutations which also confer resistance can occur outside this region and may involve amino acids which do not directly make contact with rifampin.
Drug Classrifamycin antibiotic
Resistance Mechanismantibiotic target alteration, antibiotic target replacement
Classification9 ontology terms | Show
Parent Term(s)5 ontology terms | Show
Sub-Term(s)
7 ontology terms | Show
Publications

Jin DJ and Gross CA. 1988. J Mol Biol 202(1): 45-58. Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. (PMID 3050121)

Mariam DH, et al. 2004. Antimicrob Agents Chemother 48(4): 1289-1294. Effect of rpoB mutations conferring rifampin resistance on fitness of Mycobacterium tuberculosis. (PMID 15047531)

Heep M, et al. 2001. J Clin Microbiol 39(1): 107-110. Frequency of rpoB mutations inside and outside the cluster I region in rifampin-resistant clinical Mycobacterium tuberculosis isolates. (PMID 11136757)

Pecavar V, et al. 2012. J Med Microbiol 61(PT 6): 780-785. High-resolution melting analysis of the single nucleotide polymorphism hot-spot region in the rpoB gene as an indicator of reduced susceptibility to rifaximin in Clostridium difficile. (PMID 22361457)

Wichelhaus TA, et al. 1999. Antimicrob Agents Chemother 43(11): 2813-2816. Molecular characterization of rpoB mutations conferring cross-resistance to rifamycins on methicillin-resistant Staphylococcus aureus. (PMID 10543773)

Mokrousov I, et al. 2003. Antimicrob Agents Chemother 47(7): 2231-2235. Allele-specific rpoB PCR assays for detection of rifampin-resistant Mycobacterium tuberculosis in sputum smears. (PMID 12821473)

Campbell PJ, et al. 2011. Antimicrob Agents Chemother 55(5): 2032-2041. Molecular detection of mutations associated with first- and second-line drug resistance compared with conventional drug susceptibility testing of Mycobacterium tuberculosis. (PMID 21300839)