tet(30)

Accession ARO:3000561
Synonym(s)tet30
DefinitionTet30 is a tetracycline efflux pump found in agrobacterium, a Gram-negative bacterium.
AMR Gene Familymajor facilitator superfamily (MFS) antibiotic efflux pump
Drug Classtetracycline antibiotic, nitroimidazole antibiotic, fosfomycin, rhodamine, lincosamide antibiotic, phenicol antibiotic, antibacterial free fatty acids, oxazolidinone antibiotic, penam, benzalkonium chloride, acridine dye, peptide antibiotic, diaminopyrimidine antibiotic, rifamycin antibiotic, isoniazid, glycylcycline, bicyclomycin, cephalosporin, nucleoside antibiotic, macrolide antibiotic, fluoroquinolone antibiotic
Resistance Mechanismantibiotic efflux
Efflux Componentefflux pump complex or subunit conferring antibiotic resistance
Classification30 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic tetracycline [Antibiotic]
+ major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family]
Publications

Luo ZQ and Farrand SK. 1999. J Bacteriol 181(2): 618-626. Cloning and characterization of a tetracycline resistance determinant present in Agrobacterium tumefaciens C58. (PMID 9882678)

Chopra I and Roberts M. 2001. Microbiol Mol Biol Rev 65(2): 232-260. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. (PMID 11381101)

Resistomes

Prevalence of tet(30) among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
No prevalence data


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 740


>gb|AAD09860.1|+|tet(30) [Agrobacterium fabrum str. C58]
MNKALIVILSTVALDAIGAGLIFPILPDILVEVTGGGDIGFLYGVMLGVFAVMQFVFSPILGALSDRFGRRPVLLLSLAGTLLDYLVMAF
SPLGWVLVVGRAMAGITSANMAVASAYITDITPAEQRAQRFGTVGAVMSLGFIIGPVIGGVIGAWWLRAPFLVAALFNGLNLFVALFVLP
ESRKAGPGKFAFKELNPLAPLVWLWNFKPLLPLVTVSVVFGLVAAIPGTIWVLYGAERFGWDSVHMGLSLSVFGVSGALAQAFLVGPLSR
RFGDLGTLMIGVGFDMLAYMLMAFANQSWMGYAVAPLFALGGVAMPALQSLVTSRVSDDQQGQLQGVLASLMSLAGIIGPVLTTAVFFST
KSIWIGTIWLVGAALYLLALPLFATVKTPKAVAA


>gb|AF090987|+|1-1185|tet(30) [Agrobacterium fabrum str. C58]
ATGAACAAGGCCCTTATCGTTATTCTCTCAACCGTTGCCCTCGACGCCATTGGCGCAGGCCTGATCTTCCCGATCCTGCCGGACATCTTG
GTCGAGGTGACTGGCGGCGGCGACATCGGGTTCCTCTATGGGGTCATGCTGGGGGTATTCGCCGTCATGCAATTTGTGTTCTCGCCGATC
CTTGGTGCGCTCAGCGACCGGTTCGGTCGGCGCCCGGTCTTGTTGCTTTCTTTGGCCGGTACCCTGCTTGATTACCTTGTTATGGCATTT
TCCCCGCTCGGCTGGGTGCTCGTCGTCGGGCGGGCCATGGCGGGGATCACCAGCGCAAATATGGCGGTGGCAAGCGCCTACATCACTGAC
ATCACCCCAGCCGAGCAGCGCGCGCAGCGGTTTGGCACGGTTGGTGCCGTGATGAGCCTGGGCTTTATCATCGGTCCCGTCATTGGTGGC
GTCATTGGCGCCTGGTGGCTTCGGGCACCATTTCTTGTGGCAGCCCTGTTCAATGGCCTCAACCTGTTCGTCGCGCTGTTTGTTCTGCCG
GAAAGCCGAAAGGCCGGTCCGGGCAAGTTTGCGTTCAAGGAACTTAACCCGTTGGCGCCATTGGTGTGGCTTTGGAATTTCAAGCCGCTC
CTGCCACTTGTAACCGTCTCTGTCGTCTTCGGTCTGGTGGCCGCCATCCCGGGAACGATCTGGGTGCTCTATGGCGCCGAGCGGTTCGGA
TGGGATTCGGTGCATATGGGCCTGTCGCTATCGGTTTTCGGCGTCAGTGGCGCCCTGGCGCAGGCCTTTCTCGTCGGGCCGCTCTCGCGC
CGCTTTGGTGATTTGGGCACGTTGATGATCGGCGTTGGCTTTGACATGCTGGCTTATATGCTGATGGCCTTCGCCAACCAGAGCTGGATG
GGCTACGCGGTAGCGCCCCTGTTTGCATTGGGCGGCGTTGCCATGCCGGCGCTGCAATCTCTGGTAACCAGCCGCGTGAGCGATGATCAG
CAGGGCCAGTTGCAGGGCGTGCTCGCCAGCCTCATGAGCCTGGCGGGTATAATAGGGCCGGTGCTGACCACCGCAGTGTTCTTTTCCACC
AAAAGCATCTGGATCGGGACGATCTGGCTGGTGGGTGCCGCACTTTATCTTCTCGCCTTGCCGCTGTTCGCAACGGTGAAAACCCCGAAG
GCTGTGGCGGCTTAA