| Accession | ARO:3002627 |
| CARD Short Name | aadK |
| Definition | aadK is a chromosomal-encoded aminoglycoside nucleotidyltransferase gene in B. subtilis and Bacillus spp. This enzyme confers low-level resistance to streptomycin. |
| AMR Gene Family | ANT(6) |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Bacillus subtilisg+wgs+gi |
| Resistomes with Sequence Variants | Bacillus halotoleransg+wgs+gi, Bacillus subtilisg+wgs+gi, Bacillus tequilensisg+wgs+gi |
| Classification | 11 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + determinant of antibiotic resistance + antibiotic inactivation enzyme + aminoglycoside modifying enzyme + antibiotic molecule + nucleotidylation of antibiotic conferring resistance + aminoglycoside nucleotidyltransferase (ANT) + aminoglycoside antibiotic [Drug Class] + ANT(6) [AMR Gene Family] |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Noguchi N, et al. 1993. FEMS Microbiol Lett 114(1): 47-52. Genetic mapping in Bacillus subtilis 168 of the aadK gene which encodes aminoglycoside 6-adenylyltransferase. (PMID 8293959) Ohmiya K, et al. 1989. Gene 78(2): 377-378. Nucleotide sequence of the chromosomal gene coding for the aminoglycoside 6-adenylyltransferase from Bacillus subtilis Marburg 168. (PMID 2550327) |
Prevalence of aadK among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Bacillus halotolerans | 45.45% | 0% | 32.43% | 100% |
| Bacillus subtilis | 93.55% | 0% | 72.83% | 82.19% |
| Bacillus tequilensis | 100% | 0% | 66.67% | 100% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 500