| Accession | ARO:3002635 |
| Synonym(s) | aph(2'')-Ib |
| CARD Short Name | APH(2'')-IIa |
| Definition | APH(2'')-IIa is a chromosomal-encoded aminoglycoside phosphotransferase in E. faecium and E. coli. |
| AMR Gene Family | APH(2'') |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Sequence Variants | Campylobacter colip+wgs, Campylobacter jejuniwgs, Citrobacter freundiip, Enterobacter hormaecheiwgs, Enterobacter kobeiwgs, Enterocloster clostridioformiswgs, Enterococcus faecaliswgs, Enterococcus faeciump+wgs, Histophilus somniwgs, Klebsiella pneumoniaep+wgs, Pseudomonas aeruginosawgs, Salmonella entericawgs |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + determinant of antibiotic resistance + antibiotic inactivation enzyme + aminoglycoside modifying enzyme + antibiotic molecule + phosphorylation of antibiotic conferring resistance + aminoglycoside phosphotransferase (APH) + aminoglycoside antibiotic [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Kao SJ, et al. 2000. Antimicrob Agents Chemother 44(10): 2876-2879. Detection of the high-level aminoglycoside resistance gene aph(2)-Ib in Enterococcus faecium. (PMID 10991878) |
Prevalence of APH(2'')-IIa among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Campylobacter coli | 0% | 0.91% | 0.35% | 0% |
| Campylobacter jejuni | 0% | 0% | 0.09% | 0% |
| Citrobacter freundii | 0% | 0.31% | 0% | 0% |
| Enterobacter hormaechei | 0% | 0% | 0.13% | 0% |
| Enterobacter kobei | 0% | 0% | 0.44% | 0% |
| Enterocloster clostridioformis | 0% | 0% | 2.33% | 0% |
| Enterococcus faecalis | 0% | 0% | 0.04% | 0% |
| Enterococcus faecium | 0% | 0.06% | 0.07% | 0% |
| Histophilus somni | 0% | 0% | 3.45% | 0% |
| Klebsiella pneumoniae | 0% | 0.03% | 0.18% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0.01% | 0% |
| Salmonella enterica | 0% | 0% | 0.01% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 550
