ROB-1

Accession ARO:3002995
CARD Short NameROB-1
DefinitionROB-1 is a beta-lactamase found in Pasteurella and Haemophilus.
AMR Gene FamilyROB beta-lactamase
Drug Classpenam, cephalosporin
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesActinobacillus indolicusg+wgs+gi, Actinobacillus pleuropneumoniaep+wgs, Glaesserella parasuisg+wgs+gi, Haemophilus influenzaewgs, Histophilus somniwgs, Neisseria meningitidiswgs, Pasteurella multocidag+wgs
Resistomes with Sequence VariantsActinobacillus indolicusg+wgs+gi, Actinobacillus pleuropneumoniaep+wgs, Glaesserella parasuisg+wgs+gi, Haemophilus influenzaewgs, Histophilus somniwgs, Neisseria meningitidiswgs, Pasteurella multocidag+wgs
Classification14 ontology terms | Show
Parent Term(s)1 ontology terms | Show
+ ROB beta-lactamase [AMR Gene Family]
Publications

Livrelli V, et al. 1991. Antimicrob Agents Chemother 35(2): 242-251. Sequence and molecular characterization of the ROB-1 beta-lactamase gene from Pasteurella haemolytica. (PMID 2024956)

San Millan A, et al. 2007. Antimicrob Agents Chemother 51(6): 2260-2264. Beta-lactam resistance in Haemophilus parasuis Is mediated by plasmid pB1000 bearing blaROB-1. (PMID 17438055)

Resistomes

Prevalence of ROB-1 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Actinobacillus indolicus100%0%100%100%
Actinobacillus pleuropneumoniae0%10%7.14%0%
Glaesserella parasuis19.23%0%6.15%100%
Haemophilus influenzae0%0%0.27%0%
Histophilus somni0%0%10.34%0%
Neisseria meningitidis0%0%0.28%0%
Pasteurella multocida0.71%0%1.12%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 600


>gb|CAA37052.1|+|ROB-1 [Mannheimia haemolytica]
MLNKLKIGTLLLLTLTACSPNSVHSVTSNPQPASAPVQQSATQATFQQTLANLEQQYQARIGVYVWDTETGHSLSYRADERFAYASTFKA
LLAGAVLQSLPEKDLNRTISYSQKDLVSYSPETQKYVGKGMTIAQLCEAAVRFSDNSATNLLLKELGGVEQYQRILRQLGDNVTHTNRLE
PDLNQAKPNDIRDTSTPKQMAMNLNAYLLGNTLTESQKTILWNWLDNNATGNPLIRAATPTSWKVYDKSGAGKYGVRNDIAVVRIPNRKP
IVMAIMSTQFTEEAKFNNKLVEDAAKQVFHTLQLN


>gb|X52872.1|+|221-1138|ROB-1 [Mannheimia haemolytica]
ATGTTAAATAAGTTAAAAATCGGCACATTATTATTGCTGACATTAACGGCTTGTTCGCCCAATTCTGTTCATTCGGTAACGTCTAATCCG
CAGCCTGCTAGTGCGCCTGTGCAACAATCAGCCACACAAGCCACCTTTCAACAGACTTTGGCGAATTTGGAACAGCAGTATCAAGCCCGA
ATTGGCGTTTATGTATGGGATACAGAAACGGGACATTCTTTGTCTTATCGTGCAGATGAACGCTTTGCTTATGCGTCCACTTTCAAGGCG
TTGTTGGCTGGGGCGGTGTTGCAATCGCTGCCTGAAAAAGATTTAAATCGTACCATTTCATATAGCCAAAAAGATTTGGTTAGTTATTCT
CCCGAAACCCAAAAATACGTTGGCAAAGGCATGACGATTGCCCAATTATGTGAAGCAGCCGTGCGGTTTAGCGACAACAGCGCGACCAAT
TTGCTGCTCAAAGAATTGGGTGGCGTGGAACAATATCAACGTATTTTGCGACAATTAGGCGATAACGTAACCCATACCAATCGGCTAGAA
CCCGATTTAAATCAAGCCAAACCCAACGATATTCGTGATACGAGTACACCCAAACAAATGGCGATGAATTTAAATGCGTATTTATTGGGC
AACACATTAACCGAATCGCAAAAAACGATTTTGTGGAATTGGTTGGACAATAACGCAACAGGCAATCCATTGATTCGCGCTGCTACGCCA
ACATCGTGGAAAGTGTACGATAAAAGCGGGGCGGGTAAATATGGTGTACGCAATGATATTGCGGTGGTTCGCATACCAAATCGCAAACCG
ATTGTGATGGCAATCATGAGTACGCAATTTACCGAAGAAGCCAAATTCAACAATAAATTAGTAGAAGATGCAGCAAAGCAAGTATTTCAT
ACTTTACAGCTCAACTAA