| Accession | ARO:3003741 |
| CARD Short Name | mphE |
| Definition | mphE is a macrolide phosphotransferase and resistance gene identified on a plasmid, pRSB105. |
| AMR Gene Family | macrolide phosphotransferase (MPH) |
| Drug Class | macrolide antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Acinetobacter baumanniig+p+wgs, Acinetobacter defluviip, Acinetobacter indicusp+wgs, Acinetobacter johnsoniip+wgs, Acinetobacter juniip+wgs, Acinetobacter lwoffiip+wgs, Acinetobacter nosocomialisp+wgs, Acinetobacter pittiip+wgs, Acinetobacter radioresistenswgs, Acinetobacter townerip+wgs, Acinetobacter wuhouensisp+wgs, Aeromonas caviaeg+p+wgs, Aeromonas hydrophilap+wgs, Aeromonas veroniig+p+wgs, Citrobacter freundiip+wgs, Citrobacter portucalensisp+wgs, Citrobacter werkmaniiwgs, Citrobacter youngaep+wgs, Comamonas testosteronip+wgs, Enterobacter asburiaewgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeig+wgs, Enterobacter roggenkampiiwgs, Escherichia colig+p+wgs, Klebsiella aerogenesp+wgs, Klebsiella huaxiensisp, Klebsiella michiganensisp+wgs, Klebsiella oxytocap+wgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaep+wgs, Leclercia adecarboxylatag+p, Moraxella osloensisg+wgs, Morganella morganiig+wgs, Proteus mirabilisg+p+wgs, Proteus penneriwgs, Proteus vulgarisp+wgs, Providencia rettgerig+p+wgs, Providencia stuartiig+p+wgs, Pseudomonas aeruginosag+p+wgs, Pseudomonas putidag+wgs, Pseudomonas stutzeriwgs, Raoultella planticolawgs, Salmonella entericag+p+wgs, Serratia marcescensp+wgs, Shewanella putrefaciensp, Shigella boydiiwgs, Shigella flexneriwgs, Vibrio choleraeg+p+wgs, Vibrio fluvialiswgs |
| Resistomes with Sequence Variants | Acinetobacter baumanniig+p+wgs, Acinetobacter defluviip, Acinetobacter haemolyticusp, Acinetobacter indicusp+wgs, Acinetobacter johnsoniip+wgs, Acinetobacter juniip+wgs, Acinetobacter lwoffiip+wgs, Acinetobacter nosocomialisp+wgs, Acinetobacter pittiip+wgs, Acinetobacter radioresistenswgs, Acinetobacter townerip+wgs, Acinetobacter wuhouensisp+wgs, Aeromonas caviaeg+p+wgs, Aeromonas hydrophilap+wgs, Aeromonas veroniig+p+wgs, Citrobacter freundiip+wgs, Citrobacter portucalensisp+wgs, Citrobacter werkmaniiwgs, Citrobacter youngaep+wgs, Comamonas testosteronip+wgs, Enterobacter asburiaewgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeig+wgs, Enterobacter roggenkampiiwgs, Escherichia colig+p+wgs, Klebsiella aerogenesp+wgs, Klebsiella huaxiensisp, Klebsiella michiganensisp+wgs, Klebsiella oxytocap+wgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaep+wgs, Leclercia adecarboxylatag+p, Moraxella osloensisg+wgs, Morganella morganiig+wgs, Pasteurella multocidag, Proteus mirabilisg+p+wgs, Proteus penneriwgs, Proteus vulgarisp+wgs, Providencia rettgerig+p+wgs, Providencia stuartiig+p+wgs, Pseudomonas aeruginosag+p+wgs, Pseudomonas putidag+wgs, Pseudomonas stutzeriwgs, Raoultella planticolawgs, Salmonella entericag+p+wgs, Serratia marcescensp+wgs, Shewanella putrefaciensp, Shigella boydiiwgs, Shigella flexneriwgs, Vibrio choleraeg+p+wgs, Vibrio fluvialiswgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + antibiotic molecule + macrolide inactivation enzyme + phosphorylation of antibiotic conferring resistance + macrolide antibiotic [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show + confers_resistance_to_antibiotic erythromycin [Antibiotic] + macrolide phosphotransferase (MPH) [AMR Gene Family] |
| Publications | Schluter A, et al. 2007. Appl Environ Microbiol 73(6): 1952-1960. Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function. (PMID 17261525) Pawlowski AC, et al. 2018. Nat Commun 9(1):112 The evolution of substrate discrimination in macrolide antibiotic resistance enzymes. (PMID 29317655) |
Prevalence of mphE among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 36.11% | 3.22% | 32.27% | 0% |
| Acinetobacter defluvii | 0% | 12.5% | 0% | 0% |
| Acinetobacter haemolyticus | 0% | 2.44% | 0% | 0% |
| Acinetobacter indicus | 0% | 9.43% | 2.6% | 0% |
| Acinetobacter johnsonii | 0% | 4.17% | 20% | 0% |
| Acinetobacter junii | 0% | 50% | 10.45% | 0% |
| Acinetobacter lwoffii | 0% | 1.39% | 5.26% | 0% |
| Acinetobacter nosocomialis | 0% | 3.28% | 4.02% | 0% |
| Acinetobacter pittii | 0% | 5.42% | 9.38% | 0% |
| Acinetobacter radioresistens | 0% | 0% | 3.51% | 0% |
| Acinetobacter towneri | 0% | 37.5% | 15.38% | 0% |
| Acinetobacter wuhouensis | 0% | 13.64% | 100% | 0% |
| Aeromonas caviae | 2.27% | 1.3% | 0.54% | 0% |
| Aeromonas hydrophila | 0% | 2.6% | 0.81% | 0% |
| Aeromonas veronii | 3.64% | 1.54% | 0.56% | 0% |
| Citrobacter freundii | 0% | 0.92% | 3.29% | 0% |
| Citrobacter portucalensis | 0% | 2.94% | 3.6% | 0% |
| Citrobacter werkmanii | 0% | 0% | 17.95% | 0% |
| Citrobacter youngae | 0% | 9.09% | 6.25% | 0% |
| Comamonas testosteroni | 0% | 50% | 7.14% | 0% |
| Enterobacter asburiae | 0% | 0% | 0.79% | 0% |
| Enterobacter cloacae | 0% | 1.12% | 1.6% | 0% |
| Enterobacter hormaechei | 0.72% | 0.97% | 2.16% | 0% |
| Enterobacter kobei | 4.55% | 0% | 6.99% | 0% |
| Enterobacter roggenkampii | 0% | 0% | 2.16% | 0% |
| Escherichia coli | 0.12% | 0.13% | 0.31% | 0% |
| Klebsiella aerogenes | 0% | 1.09% | 0.28% | 0% |
| Klebsiella huaxiensis | 0% | 7.69% | 0% | 0% |
| Klebsiella michiganensis | 0% | 1.71% | 1.33% | 0% |
| Klebsiella oxytoca | 0% | 1.37% | 0.84% | 0% |
| Klebsiella pneumoniae | 1.3% | 1.81% | 3.14% | 0% |
| Klebsiella quasipneumoniae | 0% | 0.85% | 8.68% | 0% |
| Leclercia adecarboxylata | 7.14% | 4.76% | 0% | 0% |
| Moraxella osloensis | 10% | 0% | 7.14% | 0% |
| Morganella morganii | 3.85% | 0% | 4.29% | 0% |
| Pasteurella multocida | 3.57% | 0% | 0% | 0% |
| Proteus mirabilis | 12.84% | 8.75% | 3.63% | 0% |
| Proteus penneri | 0% | 0% | 12.5% | 0% |
| Proteus vulgaris | 0% | 22.22% | 16.67% | 0% |
| Providencia rettgeri | 14.71% | 8.11% | 14.65% | 0% |
| Providencia stuartii | 6.25% | 11.36% | 6.82% | 0% |
| Pseudomonas aeruginosa | 0.46% | 4.09% | 0.53% | 0% |
| Pseudomonas putida | 1.41% | 0% | 0.53% | 0% |
| Pseudomonas stutzeri | 0% | 0% | 2.29% | 0% |
| Raoultella planticola | 0% | 0% | 5.13% | 0% |
| Salmonella enterica | 0.57% | 0.27% | 0.44% | 0% |
| Serratia marcescens | 0% | 1.29% | 0.39% | 0% |
| Shewanella putrefaciens | 0% | 20% | 0% | 0% |
| Shigella boydii | 0% | 0% | 1.11% | 0% |
| Shigella flexneri | 0% | 0% | 0.16% | 0% |
| Vibrio cholerae | 0.45% | 10.53% | 0.89% | 0% |
| Vibrio fluvialis | 0% | 0% | 4% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 500
Type of Antibiotic Resistance: Acquired