| Accession | ARO:3003952 |
| CARD Short Name | YojI |
| Definition | YojI mediates resistance to the peptide antibiotic microcin J25 when it is expressed from a multicopy vector. YojI is capable of pumping out microcin molecules. The outer membrane protein TolC in addition to YojI is required for export of microcin J25 out of the cell. Microcin J25 is thus the first known substrate for YojI. |
| AMR Gene Family | ATP-binding cassette (ABC) antibiotic efflux pump |
| Drug Class | peptide antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Perfect Matches | Escherichia colig+wgs, Shigella boydiig+wgs, Shigella dysenteriaewgs, Shigella flexnerig+wgs, Shigella sonneiwgs |
| Resistomes with Sequence Variants | Escherichia colig+p+wgs, Escherichia fergusoniig+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Classification | 7 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + determinant of antibiotic resistance + antibiotic molecule + peptide antibiotic [Drug Class] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] |
| Parent Term(s) | 2 ontology terms | Show + ATP-binding cassette (ABC) antibiotic efflux pump [AMR Gene Family] + confers_resistance_to_antibiotic microcin J25 [Antibiotic] |
| Publications | Delgado MA, et al. 2005. J. Bacteriol. 187(10):3465-70 YojI of Escherichia coli functions as a microcin J25 efflux pump. (PMID 15866933) |
Prevalence of YojI among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Escherichia coli | 67.6% | 0.06% | 61.36% | 0% |
| Escherichia fergusonii | 85.25% | 0% | 43.48% | 0% |
| Shigella boydii | 100% | 0% | 98.89% | 0% |
| Shigella dysenteriae | 100% | 0% | 100% | 0% |
| Shigella flexneri | 100% | 0% | 81.68% | 0% |
| Shigella sonnei | 100% | 0% | 94.38% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 1050