| Accession | ARO:3004089 |
| Synonym(s) | aadA1-pm ANT(9)(3'') |
| CARD Short Name | ANT(3'')-IIa |
| Definition | ANT(3'')-IIa is a aminoglycoside nucleotidyltransferase identified in Acinetobacter spp. via horizontal gene transfer mechanisms. |
| AMR Gene Family | ANT(3'') |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Sequence Variants | Acinetobacter baumanniig+p+wgs, Acinetobacter pittiiwgs, Aeromonas caviaewgs, Aeromonas hydrophilag+p+wgs, Aeromonas veroniiwgs, Alcaligenes faecalisg, Citrobacter freundiip+wgs, Citrobacter koseriwgs, Citrobacter portucalensisp+wgs, Citrobacter werkmaniiwgs, Citrobacter youngaewgs, Deinococcus radioduransp, Enterobacter asburiaewgs, Enterobacter chengduensiswgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheip+wgs, Enterobacter kobeip+wgs, Enterobacter roggenkampiiwgs, Escherichia colig+p+wgs, Escherichia fergusoniip+wgs, Klebsiella aerogeneswgs, Klebsiella michiganensisp+wgs, Klebsiella oxytocap+wgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaep+wgs, Leclercia adecarboxylatawgs, Morganella morganiig+wgs, Neisseria brasiliensisg, Proteus mirabilisp+wgs, Proteus vulgarisp+wgs, Providencia alcalifaciensg, Providencia rettgeriwgs, Providencia stuartiip+wgs, Pseudomonas aeruginosag+p+wgs, Pseudomonas putidap, Pseudomonas stutzeriwgs, Raoultella planticolawgs, Riemerella anatipestiferwgs, Salmonella entericap+wgs, Serratia marcescensp+wgs, Shewanella putrefaciensg+p, Shigella flexnerip, Shigella sonneiwgs, Vibrio alginolyticuswgs, Vibrio choleraeg+wgs, Vibrio fluvialiswgs, Vibrio parahaemolyticuswgs, Yersinia enterocoliticawgs |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + aminoglycoside modifying enzyme + nucleotidylation of antibiotic conferring resistance + antibiotic molecule + aminoglycoside nucleotidyltransferase (ANT) + aminoglycoside antibiotic [Drug Class] |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic spectinomycin [Antibiotic] + confers_resistance_to_antibiotic streptomycin [Antibiotic] + ANT(3'') [AMR Gene Family] |
| Publications | Zhang G, et al. 2017. PLoS Genet. 13(2):e1006602 A new subclass of intrinsic aminoglycoside nucleotidyltransferases, ANT(3'')-II, is horizontally transferred among Acinetobacter spp. by homologous recombination. (PMID 28152054) |
Prevalence of ANT(3'')-IIa among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 0.35% | 0.05% | 0.58% | 0% |
| Acinetobacter pittii | 0% | 0% | 0.28% | 0% |
| Aeromonas caviae | 0% | 0% | 3.76% | 0% |
| Aeromonas hydrophila | 3.08% | 2.6% | 0.81% | 0% |
| Aeromonas veronii | 0% | 0% | 1.12% | 0% |
| Alcaligenes faecalis | 5% | 0% | 0% | 0% |
| Citrobacter freundii | 0% | 0.31% | 4.06% | 0% |
| Citrobacter koseri | 0% | 0% | 0.9% | 0% |
| Citrobacter portucalensis | 0% | 2.94% | 0.9% | 0% |
| Citrobacter werkmanii | 0% | 0% | 10.26% | 0% |
| Citrobacter youngae | 0% | 0% | 12.5% | 0% |
| Deinococcus radiodurans | 0% | 3.7% | 0% | 0% |
| Enterobacter asburiae | 0% | 0% | 3.95% | 0% |
| Enterobacter chengduensis | 0% | 0% | 12% | 0% |
| Enterobacter cloacae | 0% | 2.23% | 3.83% | 0% |
| Enterobacter hormaechei | 0% | 1.35% | 6.47% | 0% |
| Enterobacter kobei | 0% | 0.69% | 3.93% | 0% |
| Enterobacter roggenkampii | 0% | 0% | 1.8% | 0% |
| Escherichia coli | 0.02% | 0.58% | 1.23% | 0% |
| Escherichia fergusonii | 0% | 1.07% | 3.26% | 0% |
| Klebsiella aerogenes | 0% | 0% | 0.28% | 0% |
| Klebsiella michiganensis | 0% | 1.14% | 1.06% | 0% |
| Klebsiella oxytoca | 0% | 2.74% | 3.36% | 0% |
| Klebsiella pneumoniae | 0.06% | 0.54% | 3.18% | 0% |
| Klebsiella quasipneumoniae | 0% | 0.42% | 6.32% | 0% |
| Leclercia adecarboxylata | 0% | 0% | 2.33% | 0% |
| Morganella morganii | 1.92% | 0% | 1.84% | 0% |
| Neisseria brasiliensis | 100% | 0% | 0% | 0% |
| Proteus mirabilis | 0% | 3.75% | 3.47% | 0% |
| Proteus vulgaris | 0% | 22.22% | 11.11% | 0% |
| Providencia alcalifaciens | 9.09% | 0% | 0% | 0% |
| Providencia rettgeri | 0% | 0% | 15.29% | 0% |
| Providencia stuartii | 0% | 13.64% | 4.55% | 0% |
| Pseudomonas aeruginosa | 2.91% | 0.58% | 1.25% | 0% |
| Pseudomonas putida | 0% | 4% | 0% | 0% |
| Pseudomonas stutzeri | 0% | 0% | 6.11% | 0% |
| Raoultella planticola | 0% | 0% | 2.56% | 0% |
| Riemerella anatipestifer | 0% | 0% | 0.69% | 0% |
| Salmonella enterica | 0% | 0.98% | 2.65% | 0% |
| Serratia marcescens | 0% | 1.29% | 1.44% | 0% |
| Shewanella putrefaciens | 11.11% | 20% | 0% | 0% |
| Shigella flexneri | 0% | 1.61% | 0% | 0% |
| Shigella sonnei | 0% | 0% | 0.58% | 0% |
| Vibrio alginolyticus | 0% | 0% | 0.4% | 0% |
| Vibrio cholerae | 0.45% | 0% | 1.73% | 0% |
| Vibrio fluvialis | 0% | 0% | 4% | 0% |
| Vibrio parahaemolyticus | 0% | 0% | 0.1% | 0% |
| Yersinia enterocolitica | 0% | 0% | 0.45% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 400