| Accession | ARO:3004836 |
| CARD Short Name | Ngon_23S_AZM |
| Definition | Point mutation in the 23S rRNA of Neisseria gonorrhoea shown to confer resistance to azithromycin, a macrolide type antibiotic. |
| AMR Gene Family | 23S rRNA with mutation conferring resistance to macrolide antibiotics |
| Drug Class | macrolide antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Resistomes with Sequence Variants | Neisseria gonorrhoeaeg+wgs |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + mutation conferring antibiotic resistance + determinant of antibiotic resistance + antibiotic resistant gene variant or mutant + rRNA with mutation conferring antibiotic resistance + antibiotic molecule + 23S rRNA with mutation conferring antibiotic resistance + macrolide antibiotic [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show + confers_resistance_to_antibiotic azithromycin [Antibiotic] + 23S rRNA with mutation conferring resistance to macrolide antibiotics [AMR Gene Family] |
| Publications | Bercot B, et al. 2014. Euro Surveill. 19(44): High-level azithromycin-resistant Neisseria gonorrhoeae clinical isolate in France, March 2014. (PMID 25394255) Chisholm SA, et al. 2010. Antimicrob. Agents Chemother. 54(9):3812-6 High-level azithromycin resistance occurs in Neisseria gonorrhoeae as a result of a single point mutation in the 23S rRNA genes. (PMID 20585125) Chisholm SA, et al. 2016. Sex Transm Infect 92(5):365-7 An outbreak of high-level azithromycin resistant Neisseria gonorrhoeae in England. (PMID 26601852) Pham CD, et al. 2019. Antimicrob. Agents Chemother. 63(4): Emergence of Neisseria gonorrhoeae Strains Harboring a Novel Combination of Azithromycin-Attenuating Mutations. (PMID 30917979) Zarantonelli L, et al. 1999. Antimicrob. Agents Chemother. 43(10):2468-72 Decreased azithromycin susceptibility of Neisseria gonorrhoeae due to mtrR mutations. (PMID 10508026) Singh AE, et al. 2021. Emerg Infect Dis 27(6):1718-1722 Molecular Characterization and Antimicrobial Resistance in Neisseria gonorrhoeae, Nunavut Region of Inuit Nunangat, Canada, 2018-2019. (PMID 34013864) |
Prevalence of Neisseria gonorrhoeae 23S rRNA with mutation conferring resistance to azithromycin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| Neisseria gonorrhoeae | 2.5% | 0% | 0.56% | 0% | 0% |
Model Type: rRNA gene variant model
Model Definition: Ribosomal RNA (rRNA) Gene Variant Models (RVM) are similar to Protein Variant Models (PVM), i.e. detect sequences based on their similarity to a curated reference sequence and secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles, except RVMs are designed to detect AMR acquired via mutation of genes encoding ribosomal RNAs (rRNA). RVMs include a rRNA reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTN bit-score above the curated BLASTN cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTN bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastN): 5300
PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).| Mutation | Mutation type | PubMed |
|---|---|---|
| ggataaaaagtcttttt:instt | disruptive mutation in regulatory element | PMID:10508026 |
| ggataaaaagtcttttt:dela | disruptive mutation in regulatory element | PMID:10508026 |
| a2045g | single resistance variant | PMID:30917979 |
| a2059g | single resistance variant | PMID:34013864 |
| a2145g | single resistance variant | PMID:26601852 |
| c2597t | single resistance variant | PMID:20585125 |
| c2600t | single resistance variant | PMID:25394255 |
| c2611t | single resistance variant | PMID:34013864 |
| Curator | Description | Most Recent Edit |
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