| Accession | ARO:3002601 |
| Synonym(s) | aadA1-pm aadA, aadA1, aad(3'')(9) |
| CARD Short Name | aadA |
| Definition | ANT(3'')-Ia is an aminoglycoside nucleotidyltransferase gene encoded by plasmids, transposons, integrons in Enterobacteriaceae, A. baumannii, P. aeruginosa and Vibrio cholerae. |
| AMR Gene Family | ANT(3'') |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Acinetobacter baumanniig+p+wgs+gi, Acinetobacter indicusg+p, Acinetobacter juniiwgs, Acinetobacter townerig, Actinobacillus indolicusg+gi, Aeromonas caviaep+wgs+gi, Aeromonas hydrophilap+wgs, Aeromonas veroniiwgs, Alcaligenes faecaliswgs, Bordetella trematumg, Burkholderia glumaeg, Citrobacter freundiip+wgs, Citrobacter werkmaniiwgs, Citrobacter youngaewgs, Enterobacter asburiaewgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheip+wgs, Enterobacter kobeiwgs, Enterobacter roggenkampiiwgs, Escherichia albertiiwgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniip+wgs, Glaesserella parasuiswgs, Klebsiella aerogenesp, Klebsiella michiganensisg+wgs, Klebsiella oxytocawgs, Klebsiella pneumoniaep+wgs, Klebsiella quasipneumoniaep+wgs, Lactobacillus crispatuswgs, Morganella morganiig+wgs+gi, Parvimonas micrawgs, Prevotella intermediawgs, Proteus mirabilisg+p+wgs+gi, Proteus vulgarisp+wgs, Providencia heimbachaep, Providencia rettgerig+p+wgs+gi, Providencia stuartiig+p+wgs+gi, Pseudomonas aeruginosag+p+wgs, Pseudomonas koreensiswgs, Pseudomonas putidap+wgs, Salmonella entericag+p+wgs+gi, Serratia liquefacienswgs, Shigella dysenteriaeg+p, Shigella flexnerig+p+wgs, Shigella sonneiwgs, Sphingobium yanoikuyaewgs, Trueperella pyogeneswgs+gi, Xanthomonas oryzaeg+gi |
| Resistomes with Sequence Variants | Acinetobacter baumanniig+p+wgs+gi, Acinetobacter indicusg+p+wgs, Acinetobacter johnsoniiwgs, Acinetobacter juniiwgs, Acinetobacter lwoffiiwgs, Acinetobacter nosocomialiswgs, Acinetobacter pittiiwgs, Acinetobacter radioresistenswgs, Acinetobacter townerig+p+wgs+gi, Acinetobacter wuhouensisp, Actinobacillus indolicusg+gi, Actinobacillus pleuropneumoniaeg+wgs, Aeromonas caviaep+wgs+gi, Aeromonas hydrophilap+wgs+gi, Aeromonas veroniiwgs, Alcaligenes faecaliswgs, Anaplasma phagocytophilumwgs, Avibacterium paragallinarumg, Bordetella trematumg+gi, Burkholderia glumaeg, Citrobacter amalonaticuswgs, Citrobacter freundiig+p+wgs+gi, Citrobacter koseriwgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniiwgs, Citrobacter youngaewgs, Corynebacterium diphtheriaewgs, Cronobacter sakazakiigi, Deinococcus radioduransp, Enterobacter asburiaeg+p+wgs, Enterobacter chengduensiswgs, Enterobacter cloacaeg+p+wgs, Enterobacter hormaecheig+p+wgs+gi, Enterobacter kobeip+wgs, Enterobacter roggenkampiip+wgs, Escherichia albertiig+p+wgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniip+wgs, Glaesserella parasuiswgs, Klebsiella aerogenesp+wgs, Klebsiella michiganensisg+p+wgs, Klebsiella oxytocag+p+wgs, Klebsiella pneumoniaeg+p+wgs+gi, Klebsiella quasipneumoniaeg+p+wgs, Lactobacillus crispatuswgs, Leclercia adecarboxylatag+p, Morganella morganiig+wgs+gi, Neisseria brasiliensisgi, Parvimonas micrawgs, Pasteurella multocidag+gi, Pectobacterium parmentierig+gi, Prevotella intermediawgs, Proteus mirabilisg+p+wgs+gi, Proteus penneriwgs, Proteus vulgarisg+p+wgs+gi, Providencia heimbachaeg+p+wgs+gi, Providencia rettgerig+p+wgs+gi, Providencia stuartiig+p+wgs+gi, Pseudomonas aeruginosag+p+wgs+gi, Pseudomonas fluorescenswgs, Pseudomonas koreensiswgs, Pseudomonas monteiliiwgs, Pseudomonas putidag+p+wgs, Pseudomonas stutzerig+p+wgs, Raoultella planticolap+wgs, Salmonella entericag+p+wgs+gi, Serratia liquefacienswgs, Serratia marcescensg+p+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+p+wgs+gi, Shigella flexnerig+p+wgs+gi, Shigella sonneig+p+wgs+gi, Sphingobium yanoikuyaewgs, Streptococcus sanguiniswgs, Trueperella pyogenesg+wgs+gi, Vibrio choleraeg+wgs+gi, Vibrio fluvialiswgs, Vibrio vulnificuswgs, Xanthomonas oryzaeg+gi, Yersinia enterocoliticawgs |
| Classification | 12 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + aminoglycoside modifying enzyme + nucleotidylation of antibiotic conferring resistance + antibiotic molecule + aminoglycoside nucleotidyltransferase (ANT) + aminoglycoside antibiotic [Drug Class] + ANT(3'') [AMR Gene Family] + ANT(3'')-I |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic spectinomycin [Antibiotic] + confers_resistance_to_antibiotic streptomycin [Antibiotic] + ANT(3'')-Ia |
| Publications | Hollingshead S and Vapnek D. 1985. Plasmid 13(1): 17-30. Nucleotide sequence analysis of a gene encoding a streptomycin/spectinomycin adenylyltransferase. (PMID 2986186) |
Prevalence of aadA among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| Acinetobacter baumannii | 33.63% | 0.42% | 29.08% | 18.24% | 0% |
| Acinetobacter indicus | 4.76% | 9.43% | 3.9% | 0% | 0% |
| Acinetobacter johnsonii | 0% | 0% | 1.82% | 0% | 0% |
| Acinetobacter junii | 0% | 0% | 1.49% | 0% | 0% |
| Acinetobacter lwoffii | 0% | 0% | 2.63% | 0% | 0% |
| Acinetobacter nosocomialis | 0% | 0% | 1.15% | 0% | 0% |
| Acinetobacter pittii | 0% | 0% | 1.42% | 0% | 0% |
| Acinetobacter radioresistens | 0% | 0% | 1.75% | 0% | 0% |
| Acinetobacter towneri | 12.5% | 18.75% | 7.69% | 100% | 0% |
| Acinetobacter wuhouensis | 0% | 4.55% | 0% | 0% | 0% |
| Actinobacillus indolicus | 100% | 0% | 0% | 100% | 0% |
| Actinobacillus pleuropneumoniae | 2.78% | 0% | 4.76% | 0% | 0% |
| Aeromonas caviae | 0% | 1.3% | 25.81% | 33.33% | 0% |
| Aeromonas hydrophila | 0% | 1.3% | 1.61% | 100% | 0% |
| Aeromonas veronii | 0% | 0% | 1.69% | 0% | 0% |
| Alcaligenes faecalis | 0% | 0% | 8.82% | 0% | 0% |
| Anaplasma phagocytophilum | 0% | 0% | 5.88% | 0% | 0% |
| Avibacterium paragallinarum | 6.25% | 0% | 0% | 0% | 0% |
| Bordetella trematum | 20% | 0% | 0% | 50% | 0% |
| Burkholderia glumae | 3% | 0% | 0% | 0% | 0% |
| Citrobacter amalonaticus | 0% | 0% | 1.82% | 0% | 0% |
| Citrobacter freundii | 15.57% | 2.15% | 28.43% | 36.36% | 0% |
| Citrobacter koseri | 0% | 0% | 0.9% | 0% | 0% |
| Citrobacter portucalensis | 7.41% | 0% | 9.91% | 0% | 0% |
| Citrobacter werkmanii | 0% | 0% | 66.67% | 0% | 0% |
| Citrobacter youngae | 0% | 0% | 12.5% | 0% | 0% |
| Corynebacterium diphtheriae | 0% | 0% | 0.63% | 0% | 0% |
| Cronobacter sakazakii | 0% | 0% | 0% | 50% | 0% |
| Deinococcus radiodurans | 0% | 3.7% | 0% | 0% | 0% |
| Enterobacter asburiae | 3.23% | 0.28% | 9.49% | 0% | 0% |
| Enterobacter chengduensis | 0% | 0% | 4% | 0% | 0% |
| Enterobacter cloacae | 5.36% | 2.23% | 19.49% | 0% | 0% |
| Enterobacter hormaechei | 1.08% | 2.96% | 18.99% | 10% | 0% |
| Enterobacter kobei | 0% | 0.69% | 10.04% | 0% | 0% |
| Enterobacter roggenkampii | 0% | 0.48% | 3.6% | 0% | 0% |
| Escherichia albertii | 1.43% | 0.56% | 3.87% | 0% | 0% |
| Escherichia coli | 2.38% | 2.24% | 13.95% | 2.94% | 36.52% |
| Escherichia fergusonii | 0% | 6.41% | 13.04% | 0% | 0% |
| Glaesserella parasuis | 0% | 0% | 3.59% | 0% | 0% |
| Klebsiella aerogenes | 0% | 4.35% | 3.95% | 0% | 0% |
| Klebsiella michiganensis | 3.23% | 1.14% | 9.04% | 0% | 0% |
| Klebsiella oxytoca | 5.13% | 5.48% | 10.5% | 0% | 0% |
| Klebsiella pneumoniae | 0.53% | 2.43% | 13.08% | 1.9% | 0% |
| Klebsiella quasipneumoniae | 0.84% | 1.91% | 9.61% | 0% | 0% |
| Lactobacillus crispatus | 0% | 0% | 1.6% | 0% | 0% |
| Leclercia adecarboxylata | 7.14% | 4.76% | 0% | 0% | 0% |
| Morganella morganii | 28.85% | 0% | 16.56% | 30.77% | 0% |
| Neisseria brasiliensis | 0% | 0% | 0% | 50% | 0% |
| Parvimonas micra | 0% | 0% | 6.25% | 0% | 0% |
| Pasteurella multocida | 0.71% | 0% | 0% | 16.67% | 0% |
| Pectobacterium parmentieri | 5.88% | 0% | 0% | 100% | 0% |
| Prevotella intermedia | 0% | 0% | 4.35% | 0% | 0% |
| Proteus mirabilis | 44.95% | 5% | 47.36% | 29.63% | 0% |
| Proteus penneri | 0% | 0% | 12.5% | 0% | 0% |
| Proteus vulgaris | 27.27% | 11.11% | 16.67% | 100% | 0% |
| Providencia heimbachae | 50% | 100% | 14.29% | 100% | 0% |
| Providencia rettgeri | 38.24% | 10.81% | 33.12% | 50% | 0% |
| Providencia stuartii | 12.5% | 9.09% | 22.73% | 66.67% | 0% |
| Pseudomonas aeruginosa | 2.91% | 2.34% | 1.98% | 15.28% | 0% |
| Pseudomonas fluorescens | 0% | 0% | 2.61% | 0% | 0% |
| Pseudomonas koreensis | 0% | 0% | 4.35% | 0% | 0% |
| Pseudomonas monteilii | 0% | 0% | 14.29% | 0% | 0% |
| Pseudomonas putida | 2.82% | 12% | 2.67% | 0% | 0% |
| Pseudomonas stutzeri | 7.14% | 9.09% | 4.58% | 0% | 0% |
| Raoultella planticola | 0% | 2.33% | 7.69% | 0% | 0% |
| Salmonella enterica | 1.77% | 7.06% | 7.26% | 3.64% | 0% |
| Serratia liquefaciens | 0% | 0% | 3.17% | 0% | 0% |
| Serratia marcescens | 2.27% | 2.58% | 13.24% | 0% | 0% |
| Shigella boydii | 13.33% | 0% | 30% | 0% | 0% |
| Shigella dysenteriae | 21.43% | 9.09% | 33.33% | 25% | 0% |
| Shigella flexneri | 38% | 4.42% | 67.08% | 21.43% | 0% |
| Shigella sonnei | 48.78% | 0.97% | 72.75% | 14.29% | 0% |
| Sphingobium yanoikuyae | 0% | 0% | 5.88% | 0% | 0% |
| Streptococcus sanguinis | 0% | 0% | 1.03% | 0% | 0% |
| Trueperella pyogenes | 6.67% | 0% | 16.67% | 10% | 0% |
| Vibrio cholerae | 0.45% | 0% | 3.19% | 4.76% | 0% |
| Vibrio fluvialis | 0% | 0% | 1.33% | 0% | 0% |
| Vibrio vulnificus | 0% | 0% | 0.41% | 0% | 0% |
| Xanthomonas oryzae | 0.62% | 0% | 0% | 100% | 0% |
| Yersinia enterocolitica | 0% | 0% | 5.91% | 0% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 450
| Curator | Description | Most Recent Edit |
|---|