| Accession | ARO:3002602 |
| CARD Short Name | aadA2 |
| Definition | aadA2 is an aminoglycoside nucleotidyltransferase gene encoded by plasmids and integrons in K. pneumoniae, Salmonella spp., Corynebacterium glutamicum, C. freundii and Aeromonas spp. |
| AMR Gene Family | ANT(3'') |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Acinetobacter baumanniig+p+wgs+gi, Acinetobacter indicusg+wgs, Acinetobacter juniig+wgs, Acinetobacter lwoffiiwgs, Acinetobacter radioresistenswgs, Acinetobacter townerig+wgs, Actinobacillus pleuropneumoniaeg, Aeromonas caviaewgs, Aeromonas hydrophilawgs, Aeromonas veroniig+p+wgs+gi, Bordetella trematumgi, Burkholderia cepaciawgs, Citrobacter freundiig+p+wgs, Citrobacter koserig+wgs, Citrobacter portucalensisp+wgs, Citrobacter werkmaniiwgs, Citrobacter youngaep+wgs, Comamonas testosteronigi, Corynebacterium diphtheriaewgs, Cronobacter sakazakiip, Delftia tsuruhatensisgi, Edwardsiella tardag+wgs, Enterobacter asburiaewgs, Enterobacter chengduensiswgs, Enterobacter cloacaeg+p+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeip+wgs, Enterobacter roggenkampiip+wgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniig+p+wgs, Klebsiella aerogenesg+p+wgs+gi, Klebsiella michiganensisp+wgs, Klebsiella oxytocap+wgs, Klebsiella pneumoniaeg+p+wgs+gi, Klebsiella quasipneumoniaep+wgs, Laribacter hongkongensiswgs+gi, Leclercia adecarboxylatawgs, Morganella morganiig+wgs+gi, Proteus mirabilisg+p+wgs+gi, Proteus vulgarisp, Providencia alcalifaciensg, Providencia rettgerip+wgs, Providencia stuartiig+p+gi, Pseudomonas aeruginosawgs+gi, Pseudomonas monteiliiwgs, Pseudomonas putidag+wgs, Pseudomonas stutzeriwgs, Raoultella planticolawgs, Salmonella entericag+p+wgs+gi, Serratia marcescenswgs, Shigella boydiiwgs, Shigella dysenteriaewgs, Shigella flexnerig+p+wgs, Shigella sonneig+wgs, Stenotrophomonas maltophiliawgs, Vibrio alginolyticuswgs, Vibrio choleraeg+wgs, Vibrio fluvialisg, Vibrio parahaemolyticuswgs, Yersinia enterocoliticawgs |
| Resistomes with Sequence Variants | Acinetobacter baumanniig+p+wgs+gi, Acinetobacter indicusg+wgs, Acinetobacter juniig+wgs, Acinetobacter lwoffiiwgs, Acinetobacter radioresistenswgs, Acinetobacter townerig+wgs, Actinobacillus pleuropneumoniaeg, Aeromonas caviaeg+wgs, Aeromonas hydrophilag+wgs, Aeromonas veroniig+p+wgs+gi, Bordetella trematumg+gi, Burkholderia cepaciawgs, Citrobacter freundiig+p+wgs, Citrobacter koserig+wgs, Citrobacter portucalensisp+wgs, Citrobacter werkmaniiwgs, Citrobacter youngaep+wgs, Comamonas testosteronigi, Corynebacterium diphtheriaewgs, Cronobacter malonaticuswgs, Cronobacter sakazakiip, Delftia tsuruhatensisgi, Edwardsiella tardag+wgs, Enterobacter asburiaewgs, Enterobacter chengduensiswgs, Enterobacter cloacaeg+p+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeip+wgs, Enterobacter roggenkampiip+wgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniig+p+wgs, Klebsiella aerogenesg+p+wgs+gi, Klebsiella michiganensisp+wgs, Klebsiella oxytocap+wgs, Klebsiella pneumoniaeg+p+wgs+gi, Klebsiella quasipneumoniaep+wgs, Laribacter hongkongensiswgs+gi, Leclercia adecarboxylatawgs, Morganella morganiig+wgs+gi, Proteus mirabilisg+p+wgs+gi, Proteus penneriwgs, Proteus vulgarisp+wgs, Providencia alcalifaciensg, Providencia rettgerip+wgs, Providencia stuartiig+p+wgs+gi, Pseudomonas aeruginosawgs+gi, Pseudomonas monteiliiwgs, Pseudomonas putidag+wgs, Pseudomonas stutzeriwgs, Raoultella planticolawgs, Salmonella entericag+p+wgs+gi, Serratia marcescenswgs, Shigella boydiiwgs, Shigella dysenteriaewgs, Shigella flexnerig+p+wgs, Shigella sonneig+wgs, Stenotrophomonas maltophiliawgs, Vibrio alginolyticuswgs, Vibrio choleraeg+p+wgs, Vibrio fluvialisg, Vibrio parahaemolyticuswgs, Yersinia enterocoliticawgs |
| Classification | 12 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + aminoglycoside modifying enzyme + nucleotidylation of antibiotic conferring resistance + antibiotic molecule + aminoglycoside nucleotidyltransferase (ANT) + aminoglycoside antibiotic [Drug Class] + ANT(3'') [AMR Gene Family] + ANT(3'')-I |
| Parent Term(s) | 3 ontology terms | Show + ANT(3'')-Ia + confers_resistance_to_antibiotic streptomycin [Antibiotic] + confers_resistance_to_antibiotic spectinomycin [Antibiotic] |
| Publications | Chen YT, et al. 2007. Antimicrob Agents Chemother 51(8): 3004-3007. Sequencing and comparative genomic analysis of pK29, a 269-kilobase conjugative plasmid encoding CMY-8 and CTX-M-3 beta-lactamases in Klebsiella pneumoniae. (PMID 17526756) |
Prevalence of aadA2 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 3.01% | 0.42% | 2.97% | 2.52% |
| Acinetobacter indicus | 4.76% | 0% | 1.3% | 0% |
| Acinetobacter junii | 9.09% | 0% | 1.49% | 0% |
| Acinetobacter lwoffii | 0% | 0% | 5.26% | 0% |
| Acinetobacter radioresistens | 0% | 0% | 3.51% | 0% |
| Acinetobacter towneri | 12.5% | 0% | 3.85% | 0% |
| Actinobacillus pleuropneumoniae | 2.78% | 0% | 0% | 0% |
| Aeromonas caviae | 4.55% | 0% | 8.6% | 0% |
| Aeromonas hydrophila | 4.62% | 0% | 4.84% | 0% |
| Aeromonas veronii | 5.45% | 3.08% | 2.81% | 12.5% |
| Bordetella trematum | 20% | 0% | 0% | 50% |
| Burkholderia cepacia | 0% | 0% | 3.59% | 0% |
| Citrobacter freundii | 0.82% | 1.54% | 8.51% | 0% |
| Citrobacter koseri | 6.25% | 0% | 0.9% | 0% |
| Citrobacter portucalensis | 0% | 5.88% | 15.32% | 0% |
| Citrobacter werkmanii | 0% | 0% | 25.64% | 0% |
| Citrobacter youngae | 0% | 9.09% | 6.25% | 0% |
| Comamonas testosteroni | 0% | 0% | 0% | 100% |
| Corynebacterium diphtheriae | 0% | 0% | 0.63% | 0% |
| Cronobacter malonaticus | 0% | 0% | 1.82% | 0% |
| Cronobacter sakazakii | 0% | 2.56% | 0% | 0% |
| Delftia tsuruhatensis | 0% | 0% | 0% | 100% |
| Edwardsiella tarda | 10% | 0% | 6.67% | 0% |
| Enterobacter asburiae | 0% | 0% | 2.77% | 0% |
| Enterobacter chengduensis | 0% | 0% | 16% | 0% |
| Enterobacter cloacae | 1.79% | 3.35% | 5.43% | 0% |
| Enterobacter hormaechei | 0.36% | 1.99% | 6.52% | 0% |
| Enterobacter kobei | 0% | 1.38% | 8.3% | 0% |
| Enterobacter roggenkampii | 0% | 0.97% | 3.96% | 0% |
| Escherichia albertii | 1.43% | 0% | 0.65% | 0% |
| Escherichia coli | 1% | 2.4% | 5.74% | 1.15% |
| Escherichia fergusonii | 1.64% | 3.56% | 4.35% | 0% |
| Klebsiella aerogenes | 4% | 3.26% | 1.69% | 25% |
| Klebsiella michiganensis | 0% | 2.29% | 1.6% | 0% |
| Klebsiella oxytoca | 0% | 2.74% | 4.2% | 0% |
| Klebsiella pneumoniae | 17.46% | 4.16% | 18.3% | 34.29% |
| Klebsiella quasipneumoniae | 0% | 2.33% | 10.92% | 0% |
| Laribacter hongkongensis | 0% | 0% | 2.08% | 50% |
| Leclercia adecarboxylata | 0% | 0% | 9.3% | 0% |
| Morganella morganii | 17.31% | 0% | 6.75% | 30.77% |
| Proteus mirabilis | 41.28% | 7.5% | 6.44% | 25.93% |
| Proteus penneri | 0% | 0% | 12.5% | 0% |
| Proteus vulgaris | 0% | 11.11% | 5.56% | 0% |
| Providencia alcalifaciens | 9.09% | 0% | 0% | 0% |
| Providencia rettgeri | 0% | 2.7% | 5.1% | 0% |
| Providencia stuartii | 12.5% | 2.27% | 2.27% | 33.33% |
| Pseudomonas aeruginosa | 0% | 0% | 1.23% | 2.78% |
| Pseudomonas monteilii | 0% | 0% | 2.38% | 0% |
| Pseudomonas putida | 1.41% | 0% | 0.53% | 0% |
| Pseudomonas stutzeri | 0% | 0% | 0.76% | 0% |
| Raoultella planticola | 0% | 0% | 2.56% | 0% |
| Salmonella enterica | 3.09% | 6.18% | 2.74% | 3.31% |
| Serratia marcescens | 0% | 0% | 0.13% | 0% |
| Shigella boydii | 0% | 0% | 6.67% | 0% |
| Shigella dysenteriae | 0% | 0% | 3.33% | 0% |
| Shigella flexneri | 1% | 6.43% | 0.62% | 0% |
| Shigella sonnei | 2.44% | 0% | 1.39% | 0% |
| Stenotrophomonas maltophilia | 0% | 0% | 0.3% | 0% |
| Vibrio alginolyticus | 0% | 0% | 0.4% | 0% |
| Vibrio cholerae | 0.9% | 10.53% | 0.51% | 0% |
| Vibrio fluvialis | 2.63% | 0% | 0% | 0% |
| Vibrio parahaemolyticus | 0% | 0% | 0.1% | 0% |
| Yersinia enterocolitica | 0% | 0% | 0.91% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 450