| Accession | ARO:3003099 |
| CARD Short Name | cphA2 |
| Definition | CphA2 is an Ambler Class B MBL; subclass B2 originally isolated from Aeromonas hydrophila. This enzyme has specific activity against carbapenems and is active as a mono-zinc protein. |
| AMR Gene Family | CphA beta-lactamase |
| Drug Class | carbapenem |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Sequence Variants | Aeromonas enteropelogeneswgs, Aeromonas hydrophilag+wgs |
| Classification | 13 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + hydrolysis of antibiotic conferring resistance + hydrolysis of beta-lactam antibiotic by metallo-beta-lactamase + beta-lactamase + antibiotic molecule + beta-lactam antibiotic + class B (metallo-) beta-lactamase + subclass B2 (metallo-) beta-lactamase + carbapenem [Drug Class] |
| Parent Term(s) | 1 ontology terms | Show + CphA beta-lactamase [AMR Gene Family] |
| Publications | Kupper M, et al. 2012. Extremophiles 16(1):45-55 The CphAII protein from Aquifex aeolicus exhibits a metal-dependent phosphodiesterase activity. (PMID 22009263) Rasmussen BA, et al. 1997. Antimicrob. Agents Chemother. 41(2):223-32 Carbapenem-hydrolyzing beta-lactamases. (PMID 9021171) |
Prevalence of cphA2 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Aeromonas enteropelogenes | 0% | 0% | 12.5% | 0% |
| Aeromonas hydrophila | 35.38% | 0% | 29.03% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 450
