| Accession | ARO:3003209 |
| CARD Short Name | fosA5 |
| Definition | fosA5 is a fosfomycin resistance gene isolated from clinical strain of Escherichia coli E265. It is susceptible to amikacin, tetracycline and imipenem, and resistant to sulphonamide, cephalosporins, gentamicin, ciprofloxacin, chloramphenicol and streptomycin. |
| AMR Gene Family | fosfomycin thiol transferase |
| Drug Class | phosphonic acid antibiotic, aminoglycoside antibiotic, fluoroquinolone antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Citrobacter freundiiwgs, Enterobacter hormaecheip+wgs, Escherichia colig+wgs, Klebsiella pneumoniaeg+wgs, Serratia marcescensp |
| Resistomes with Sequence Variants | Citrobacter freundiiwgs, Cronobacter malonaticuswgs, Enterobacter hormaecheip+wgs, Escherichia colig+wgs, Klebsiella aerogenesg+wgs, Klebsiella huaxiensisg+wgs, Klebsiella michiganensisg+wgs, Klebsiella oxytocag+p+wgs, Klebsiella pneumoniaeg+wgs+gi, Klebsiella quasipneumoniaeg+wgs, Raoultella planticolag+wgs, Serratia marcescensp, Shigella flexneriwgs |
| Classification | 13 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + hydrolysis of antibiotic conferring resistance + antibiotic molecule + phosphonic acid antibiotic [Drug Class] + antibiotic mixture + fosfomycin inactivation enzyme + hydrolysis of fosfomycin epoxide ring + aminoglycoside antibiotic [Drug Class] + fluoroquinolone antibiotic [Drug Class] |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic ciprofloxacin [Antibiotic] + fosfomycin thiol transferase [AMR Gene Family] + confers_resistance_to_antibiotic gentamicin [Antibiotic] |
| Publications | Ma Y, et al. 2015. Lett Appl Microbiol 60(3): 259-264. Characterization of fosA5, a new plasmid-mediated fosfomycin resistance gene in Escherichia coli. (PMID 25441705) |
Prevalence of fosA5 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Citrobacter freundii | 0% | 0% | 0.19% | 0% |
| Cronobacter malonaticus | 0% | 0% | 5.45% | 0% |
| Enterobacter hormaechei | 0% | 0.06% | 0.22% | 0% |
| Escherichia coli | 0.02% | 0% | 0.05% | 0% |
| Klebsiella aerogenes | 92% | 0% | 75.42% | 0% |
| Klebsiella huaxiensis | 100% | 0% | 66.67% | 0% |
| Klebsiella michiganensis | 96.77% | 0% | 67.02% | 0% |
| Klebsiella oxytoca | 97.44% | 0.68% | 74.37% | 0% |
| Klebsiella pneumoniae | 14.56% | 0% | 9.5% | 1.9% |
| Klebsiella quasipneumoniae | 5.88% | 0% | 6.32% | 0% |
| Raoultella planticola | 100% | 0% | 94.87% | 0% |
| Serratia marcescens | 0% | 0.65% | 0% | 0% |
| Shigella flexneri | 0% | 0% | 0.16% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 200