| Accession | ARO:3003479 |
| CARD Short Name | tetR |
| Definition | TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites. Mutations arise within tetR results in lower affinity for tetracyclin. |
| AMR Gene Family | major facilitator superfamily (MFS) antibiotic efflux pump |
| Drug Class | tetracycline antibiotic |
| Resistance Mechanism | antibiotic efflux, antibiotic target alteration |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Efflux Regulator | protein(s) and two-component regulatory system modulating antibiotic efflux |
| Resistomes with Sequence Variants | Acinetobacter baumanniig+p+wgs+gi, Acinetobacter nosocomialiswgs, Acinetobacter radioresistenswgs, Actinobacillus indolicusg, Actinobacillus pleuropneumoniaeg+gi, Actinobacillus porcitonsillarumg+gi, Avibacterium paragallinarumg+wgs+gi, Citrobacter amalonaticuswgs, Citrobacter freundiig+wgs, Citrobacter koseriwgs, Citrobacter portucalensiswgs, Citrobacter werkmaniiwgs, Cronobacter sakazakiiwgs, Enterobacter asburiaep+wgs, Enterobacter chengduensiswgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeip+wgs, Enterobacter roggenkampiiwgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniig+p+wgs+gi, Glaesserella parasuisg+wgs, Haemophilus influenzaewgs+gi, Haemophilus parainfluenzaeg+wgs, Kingella kingaegi, Klebsiella aerogeneswgs, Klebsiella michiganensisg+p+wgs, Klebsiella oxytocawgs, Klebsiella pneumoniaeg+p+wgs+gi, Klebsiella quasipneumoniaewgs, Leclercia adecarboxylatawgs, Morganella morganiig+wgs+gi, Mycobacterium tuberculosiswgs, Neisseria meningitidisg+wgs, Pasteurella multocidag+wgs+gi, Proteus columbaeg, Proteus mirabilisg+p+wgs, Proteus pennerig+wgs, Proteus vulgarisp+wgs, Providencia alcalifaciensg+wgs, Providencia rettgerig+wgs, Providencia stuartiig+wgs, Salmonella entericag+p+wgs+gi, Shigella boydiig+wgs, Shigella dysenteriaeg+p+wgs+gi, Shigella flexnerig+p+wgs+gi, Shigella sonneip+wgs, Staphylococcus aureuswgs, Vibrio alginolyticuswgs, Vibrio choleraep+wgs, Vibrio harveyip+wgs, Vibrio owensiig+gi, Vibrio parahaemolyticusg+p+wgs+gi, Vibrio vulnificuswgs |
| Classification | 12 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic efflux [Resistance Mechanism] + antibiotic target alteration [Resistance Mechanism] + mutation conferring antibiotic resistance + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + tetracycline antibiotic [Drug Class] + major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family] + tetracycline [Antibiotic] + antibiotic resistant gene variant or mutant |
| Parent Term(s) | 3 ontology terms | Show + regulates tet(A) + mutant efflux regulatory protein conferring antibiotic resistance + protein(s) and two-component regulatory system modulating antibiotic efflux [Efflux Regulator] |
| Publications | Smith LD, et al. 1988. J Mol Biol 203(4): 949-959. Mutations in the Tn10 tet repressor that interfere with induction. Location of the tetracycline-binding domain. (PMID 3062183) |
Prevalence of tetR among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| Acinetobacter baumannii | 40.53% | 1.04% | 54.74% | 27.67% | 0% |
| Acinetobacter nosocomialis | 0% | 0% | 1.15% | 0% | 0% |
| Acinetobacter radioresistens | 0% | 0% | 3.51% | 0% | 0% |
| Actinobacillus indolicus | 100% | 0% | 0% | 0% | 0% |
| Actinobacillus pleuropneumoniae | 5.56% | 0% | 0% | 100% | 0% |
| Actinobacillus porcitonsillarum | 100% | 0% | 0% | 100% | 0% |
| Avibacterium paragallinarum | 50% | 0% | 64.71% | 100% | 0% |
| Citrobacter amalonaticus | 0% | 0% | 1.82% | 0% | 0% |
| Citrobacter freundii | 0.82% | 0% | 5.42% | 0% | 0% |
| Citrobacter koseri | 0% | 0% | 1.8% | 0% | 0% |
| Citrobacter portucalensis | 0% | 0% | 0.9% | 0% | 0% |
| Citrobacter werkmanii | 0% | 0% | 7.69% | 0% | 0% |
| Cronobacter sakazakii | 0% | 0% | 0.22% | 0% | 0% |
| Enterobacter asburiae | 0% | 0.83% | 2.37% | 0% | 0% |
| Enterobacter chengduensis | 0% | 0% | 4% | 0% | 0% |
| Enterobacter cloacae | 0% | 0.56% | 3.51% | 0% | 0% |
| Enterobacter hormaechei | 1.44% | 3.54% | 7.81% | 0% | 0% |
| Enterobacter kobei | 0% | 0.69% | 1.75% | 0% | 0% |
| Enterobacter roggenkampii | 0% | 0% | 1.8% | 0% | 0% |
| Escherichia albertii | 7.14% | 0% | 7.74% | 0% | 0% |
| Escherichia coli | 4.26% | 1.74% | 15.89% | 2.81% | 20.74% |
| Escherichia fergusonii | 6.56% | 3.91% | 10.87% | 100% | 0% |
| Glaesserella parasuis | 38.46% | 0% | 29.74% | 0% | 0% |
| Haemophilus influenzae | 0% | 0% | 0.8% | 25% | 0% |
| Haemophilus parainfluenzae | 6.25% | 0% | 4.88% | 0% | 0% |
| Kingella kingae | 0% | 0% | 0% | 100% | 0% |
| Klebsiella aerogenes | 0% | 0% | 0.28% | 0% | 0% |
| Klebsiella michiganensis | 9.68% | 2.29% | 1.6% | 0% | 0% |
| Klebsiella oxytoca | 0% | 0% | 3.78% | 0% | 0% |
| Klebsiella pneumoniae | 0.24% | 0.24% | 2.19% | 0.95% | 0% |
| Klebsiella quasipneumoniae | 0% | 0% | 0.79% | 0% | 0% |
| Leclercia adecarboxylata | 0% | 0% | 4.65% | 0% | 0% |
| Morganella morganii | 46.15% | 0% | 40.49% | 23.08% | 0% |
| Mycobacterium tuberculosis | 0% | 0% | 0.06% | 0% | 0% |
| Neisseria meningitidis | 1.53% | 0% | 7.04% | 0% | 0% |
| Pasteurella multocida | 6.43% | 0% | 6.37% | 50% | 0% |
| Proteus columbae | 100% | 0% | 0% | 0% | 0% |
| Proteus mirabilis | 0.92% | 2.5% | 1.82% | 0% | 0% |
| Proteus penneri | 50% | 0% | 12.5% | 0% | 0% |
| Proteus vulgaris | 0% | 22.22% | 16.67% | 0% | 0% |
| Providencia alcalifaciens | 18.18% | 0% | 3.45% | 0% | 0% |
| Providencia rettgeri | 2.94% | 0% | 23.57% | 0% | 0% |
| Providencia stuartii | 75% | 0% | 88.64% | 0% | 0% |
| Salmonella enterica | 7.13% | 3.12% | 11.73% | 12.25% | 0% |
| Shigella boydii | 6.67% | 0% | 18.89% | 0% | 0% |
| Shigella dysenteriae | 21.43% | 4.55% | 26.67% | 25% | 0% |
| Shigella flexneri | 47% | 0.8% | 81.37% | 23.81% | 0% |
| Shigella sonnei | 0% | 0.97% | 14.02% | 0% | 0% |
| Staphylococcus aureus | 0% | 0% | 0.01% | 0% | 0% |
| Vibrio alginolyticus | 0% | 0% | 1.19% | 0% | 0% |
| Vibrio cholerae | 0% | 10.53% | 0.06% | 0% | 0% |
| Vibrio harveyi | 0% | 14.29% | 4% | 0% | 0% |
| Vibrio owensii | 8.33% | 0% | 0% | 50% | 0% |
| Vibrio parahaemolyticus | 2.27% | 1.86% | 2.06% | 100% | 0% |
| Vibrio vulnificus | 0% | 0% | 1.22% | 0% | 0% |
Model Type: protein overexpression model
Model Definition: Protein Overexpression Models (POM) are similar to Protein Variant Models (PVM) in that they include a protein reference sequence, a curated BLASTP bitscore cut-off, and mapped resistance variants. Whereas PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, reporting only those with curated mutations conferring AMR, POMs are restricted to regulatory proteins and report both wild-type sequences and/or sequences with mutations leading to overexpression of efflux complexes. The former lead to efflux of antibiotics at basal levels, while the latter can confer clinical resistance. POMs include a protein reference sequence (often from wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Perfect RGI match is 100% identical to the wild-type reference protein sequence along its entire length, a Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value may or may not contain at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off may or may not contain at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 400
PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).| Mutation | Mutation type | PubMed |
|---|---|---|
| H64Y | single resistance variant | PMID:3062183 |
| N82H | single resistance variant | PMID:3062183 |
| T103I | single resistance variant | PMID:3062183 |
| Curator | Description | Most Recent Edit |
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