| Accession | ARO:3003479 |
| CARD Short Name | tetR |
| Definition | TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites. Mutations arise within tetR results in lower affinity for tetracyclin. |
| AMR Gene Family | major facilitator superfamily (MFS) antibiotic efflux pump |
| Drug Class | tetracycline antibiotic |
| Resistance Mechanism | antibiotic target alteration, antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Efflux Regulator | protein(s) and two-component regulatory system modulating antibiotic efflux |
| Resistomes with Sequence Variants | Acinetobacter baumanniig+p, Actinobacillus indolicusg, Actinobacillus pleuropneumoniaeg, Actinobacillus porcitonsillarumg, Avibacterium paragallinarumg, Citrobacter freundiig, Enterobacter asburiaep, Enterobacter cloacaep, Enterobacter hormaecheig+p, Enterobacter kobeip, Escherichia albertiig, Escherichia colig+p, Escherichia fergusoniig+p, Glaesserella parasuisg, Haemophilus parainfluenzaeg, Klebsiella michiganensisg+p, Klebsiella pneumoniaeg+p, Morganella morganiig, Neisseria meningitidisg, Pasteurella multocidag, Proteus columbaeg, Proteus mirabilisg+p, Proteus pennerig, Proteus vulgarisp, Providencia alcalifaciensg, Providencia rettgerig, Providencia stuartiig, Salmonella entericag+p, Shigella boydiig, Shigella dysenteriaeg+p, Shigella flexnerig+p, Shigella sonneip, Vibrio choleraep, Vibrio harveyip, Vibrio owensiig, Vibrio parahaemolyticusg+p |
| Classification | 12 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + antibiotic molecule + antibiotic efflux [Resistance Mechanism] + determinant of antibiotic resistance + tetracycline antibiotic [Drug Class] + mutation conferring antibiotic resistance + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family] + tetracycline [Antibiotic] + antibiotic resistant gene variant or mutant |
| Parent Term(s) | 3 ontology terms | Show |
| Publications | Smith LD, et al. 1988. J Mol Biol 203(4): 949-959. Mutations in the Tn10 tet repressor that interfere with induction. Location of the tetracycline-binding domain. (PMID 3062183) |
Prevalence of tetR among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 381 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 40.53% | 1.04% | 0% | 0% |
| Actinobacillus indolicus | 100% | 0% | 0% | 0% |
| Actinobacillus pleuropneumoniae | 5.56% | 0% | 0% | 0% |
| Actinobacillus porcitonsillarum | 100% | 0% | 0% | 0% |
| Avibacterium paragallinarum | 50% | 0% | 0% | 0% |
| Citrobacter freundii | 0.82% | 0% | 0% | 0% |
| Enterobacter asburiae | 0% | 0.83% | 0% | 0% |
| Enterobacter cloacae | 0% | 0.56% | 0% | 0% |
| Enterobacter hormaechei | 1.44% | 3.54% | 0% | 0% |
| Enterobacter kobei | 0% | 0.69% | 0% | 0% |
| Escherichia albertii | 7.14% | 0% | 0% | 0% |
| Escherichia coli | 4.26% | 1.74% | 0% | 0% |
| Escherichia fergusonii | 6.56% | 3.91% | 0% | 0% |
| Glaesserella parasuis | 38.46% | 0% | 0% | 0% |
| Haemophilus parainfluenzae | 6.25% | 0% | 0% | 0% |
| Klebsiella michiganensis | 9.68% | 2.29% | 0% | 0% |
| Klebsiella pneumoniae | 0.24% | 0.24% | 0% | 0% |
| Morganella morganii | 46.15% | 0% | 0% | 0% |
| Neisseria meningitidis | 1.53% | 0% | 0% | 0% |
| Pasteurella multocida | 6.43% | 0% | 0% | 0% |
| Proteus columbae | 100% | 0% | 0% | 0% |
| Proteus mirabilis | 0.92% | 2.5% | 0% | 0% |
| Proteus penneri | 50% | 0% | 0% | 0% |
| Proteus vulgaris | 0% | 22.22% | 0% | 0% |
| Providencia alcalifaciens | 18.18% | 0% | 0% | 0% |
| Providencia rettgeri | 2.94% | 0% | 0% | 0% |
| Providencia stuartii | 75% | 0% | 0% | 0% |
| Salmonella enterica | 7.13% | 3.12% | 0% | 0% |
| Shigella boydii | 6.67% | 0% | 0% | 0% |
| Shigella dysenteriae | 21.43% | 4.55% | 0% | 0% |
| Shigella flexneri | 47% | 0.8% | 0% | 0% |
| Shigella sonnei | 0% | 0.97% | 0% | 0% |
| Vibrio cholerae | 0% | 10.53% | 0% | 0% |
| Vibrio harveyi | 0% | 14.29% | 0% | 0% |
| Vibrio owensii | 8.33% | 0% | 0% | 0% |
| Vibrio parahaemolyticus | 2.27% | 1.86% | 0% | 0% |
Model Type: protein overexpression model
Model Definition: Protein Overexpression Models (POM) are similar to Protein Variant Models (PVM) in that they include a protein reference sequence, a curated BLASTP bitscore cut-off, and mapped resistance variants. Whereas PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, reporting only those with curated mutations conferring AMR, POMs are restricted to regulatory proteins and report both wild-type sequences and/or sequences with mutations leading to overexpression of efflux complexes. The former lead to efflux of antibiotics at basal levels, while the latter can confer clinical resistance. POMs include a protein reference sequence (often from wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Perfect RGI match is 100% identical to the wild-type reference protein sequence along its entire length, a Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value may or may not contain at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off may or may not contain at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 400
Legend:
Published Variants:
| PMID: 3062183 | H64Y N82H T103I |