eatAv

Accession ARO:3003761
DefinitioneatAv is a mutated form of the wildtype eatA ABC-F subfamily protein isolated from Enterococcus faecium conferring resistance to lincosamides, streptogramin A's and pleuromutilins (LSaP phenotype).
AMR Gene FamilyABC-F ATP-binding cassette ribosomal protection protein
Drug Classphenicol antibiotic, pleuromutilin antibiotic, tetracycline antibiotic, streptogramin antibiotic, lincosamide antibiotic, oxazolidinone antibiotic, macrolide antibiotic
Resistance Mechanismantibiotic target protection
Classification13 ontology terms | Show
Parent Term(s)4 ontology terms | Show
+ confers_resistance_to_drug_class lincosamide antibiotic [Drug Class]
+ confers_resistance_to_drug_class streptogramin antibiotic [Drug Class]
+ confers_resistance_to_antibiotic pleuromutilin [Antibiotic]
+ ABC-F ATP-binding cassette ribosomal protection protein [AMR Gene Family]
Publications

Isnard C, et al. 2013. Antimicrob. Agents Chemother. 57(9):4463-9 Genetic basis for in vitro and in vivo resistance to lincosamides, streptogramins A, and pleuromutilins (LSAP phenotype) in Enterococcus faecium. (PMID 23836170)

Lam MM, et al. 2012. J. Bacteriol. 194(9):2334-41 Comparative analysis of the first complete Enterococcus faecium genome. (PMID 22366422)

Resistomes

Prevalence of eatAv among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
Enterococcus faecalis0%0%0.26%
Enterococcus faecium25.6%0%36.61%
Show Perfect Only


Detection Models

Model Type: protein variant model

Model Definition: The protein variant model is an AMR detection model. Protein variant models are similar to protein homolog models - they detect the presence of a protein sequence based on its similarity to a curated reference sequence, but secondarily search submitted query sequences for curated sets of mutations shown clinically to confer resistance relative to wild-type. This model includes a protein reference sequence, a curated BLASTP cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of: single resistance variants, insertions, deletions, co-dependent resistance variants, nonsense SNPs, and/or frameshift mutations. Protein variant model matches to reference sequences are categorized on two criteria: strict and loose. A strict match has a BLASTP bitscore above the curated BLASTP cutoff value and contains at least one detected mutation from amongst the mapped resistance variants; a loose match has a BLASTP bitscore below the curated BLASTP cutoff value but still contains at least one detected mutation from amongst the mapped resistance variants. Regardless of BLASTP bitscore, if a sequence does not contain one of the mapped resistance variants, it is not considered a match and not detected by the protein variant model.

Legend:

  • discovered in clinical, agricultural, or environmental isolates
  • discovered via laboratory selection experiments


Bit-score Cut-off (blastP): 1000

PMID in progress (single): T450I


>gb|AGQ48857.1|+|eatAv [Enterococcus faecium]
MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGKTTLLNILQNKLPYQGQ
VIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQLMQTDPEILWREFSTLSGG
EKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLKKKKQGFIVVSHDRGFIDEVV
DHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEKLKKEVSRLKKTAAEKAEWSRSR
EGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSKAIVNRMETQISEKEKLLKDIEYID
SLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHFTIEPHQRVAISGPNGAGKSSIIHYLL
GAFNGKVIGEKYQPKHLSISYASQNYEDNRGTLAEFAEKNQVDYQAFLNNLRKLGMERDV
FHNKIEQMSMGQRKKVELAKSLSQPAELYIWDEPLNYLDVFNQEQLEQLILNVKPAMLLV
EHDQTFLDKVSTEIISLERI



>gb|KF010779.1|+|1-1503|eatAv [Enterococcus faecium]
ATGTCTAAAATCGAAATAAAAAATCTGACATTCGGCTACGACAGCCAAGGCACATTATTATTTGAACAAGCAAATCTAAATTTTGACACA
CAATGGAAACTAGGACTTATCGGACGAAACGGTCGAGGAAAGACAACTTTACTGAATATTCTACAAAACAAACTACCTTATCAAGGGCAA
GTAATCCATCAGCAAGAATTTGCCTATTTCCCGCAACAGACAAAAGATAAAGAACGTTTAACCTATTACGTGTTAAATGATATTACGGAT
TTTGAGATATGGGAAATCGAAAGAGAGCTCCAATTGATGCAAACAGATCCTGAAATCTTATGGAGAGAATTCAGCACACTATCGGGGGGA
GAGAAGACAAAAGTCCTACTGGCACTTTTATTTGTGGATGACACTCATTTCCCGTTAATCGATGAACCAACGAATCATTTGGATATCTCT
GGTAGAAAACAAGTAGCGGCTTATTTGAAAAAGAAAAAACAAGGCTTCATCGTGGTCAGCCATGACCGGGGATTTATCGATGAAGTAGTG
GACCATGTTTTAGCAATCGAAAAAAGTCAACTGGAACTTTATCAAGGGAATTTCTCTATCTATGAAGAACAGAAAAAACTTCGTGATGAA
TTTGAAATGGCTCAAAATGAAAAATTGAAAAAAGAAGTCAGTAGGCTAAAGAAAACAGCAGCTGAAAAAGCCGAATGGTCTCGTTCCCGA
GAAGGAGATAAAACAAAGAAACAAGTCGGATTCATCGATACTGAATCTAGACGAGTGAATAAAGGAGCAGTGGGTGCTGATGCTGCACGG
ACGATGAAACGATCCAAAGCAATCGTGAATCGGATGGAGACCCAGATCAGCGAGAAAGAAAAACTATTAAAAGATATCGAATATATCGAT
TCTTTGACGATGAATAGCCAAGCGTCTCACCATAAGCGACTTTTAAGCGTAGAAGATCTTCAATTAGGGTATGAAAATCTGTTATTCGAG
CCAATTCATTTTACAATCGAGCCTCATCAGCGGGTGGCGATTTCAGGTCCTAACGGTGCAGGAAAGTCATCCATTATCCATTATCTTCTG
GGGGCATTCAACGGCAAGGTTATAGGAGAAAAATACCAGCCAAAACATCTGAGCATTAGTTATGCAAGCCAAAATTATGAAGACAATCGA
GGAACGTTGGCGGAATTTGCAGAGAAAAACCAAGTAGACTACCAAGCATTTTTGAACAACCTCCGAAAGCTTGGGATGGAAAGAGATGTT
TTTCATAACAAGATCGAGCAGATGAGTATGGGCCAACGGAAAAAAGTGGAATTGGCTAAATCTTTATCACAGCCAGCTGAACTATATATA
TGGGATGAACCATTGAATTATTTGGATGTCTTCAATCAAGAACAATTAGAACAACTGATCTTGAACGTGAAACCTGCCATGTTACTAGTG
GAACATGATCAAACCTTCCTGGATAAAGTATCTACTGAGATTATTTCTCTTGAGAGAATCTAA