| Ontology | CARD's Antibiotic Resistance Ontology |
| Accession | ARO:3003769 |
| CARD Short Name | Saur_mprF |
| Definition | MprF is a integral membrane protein that modifies the negatively-charged phosphatidylglycerol on the membrane surface. A point mutation in the putative mprF synthase domain is associated with enhanced expression, increased synthesis and reduced daptomycin surface binding. This confers resistance to cationic peptides that disrupt the cell membrane, including defensins. |
| AMR Gene Family | defensin resistant mprF |
| Drug Class | peptide antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + charge alteration conferring antibiotic resistance + determinant of antibiotic resistance + gene altering cell wall charge + antibiotic molecule + antibiotic resistant mprF + antibiotic target modifying enzyme + peptide antibiotic [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Yang SJ, et al. 2010. Antimicrob Agents Chemother 54(8): 3079-3085. Cell wall thickening is not a universal accompaniment of the daptomycin nonsusceptibility phenotype in Staphylococcus aureus: evidence for multiple resistance mechanisms. (PMID 20498310) Staubitz P, et al. 2004. FEMS Microbiol. Lett. 231(1):67-71 MprF-mediated biosynthesis of lysylphosphatidylglycerol, an important determinant in staphylococcal defensin resistance. (PMID 14769468) Ernst CM, et al. 2009. PLoS Pathog 5(11): E1000660. The bacterial defensin resistance protein MprF consists of separable domains for lipid lysinylation and antimicrobial peptide repulsion. (PMID 19915718) |
Prevalence of Staphylococcus aureus mprF among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| No prevalence data | |||||
Model Type: protein variant model
Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 1600
PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).| Mutation | Mutation type | PubMed |
|---|---|---|
| T345A | single resistance variant | PMID:20498310 |
| Curator | Description | Most Recent Edit |
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