Listeria monocytogenes mprF

Accession ARO:3003770
CARD Short NameLmon_mprF
DefinitionMprF is a integral membrane protein that modifies the negatively-charged phosphatidylglycerol on the membrane surface. This confers resistance to cationic peptides that disrupt the cell membrane, including defensins.
AMR Gene Familydefensin resistant mprF
Drug Classpeptide antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Perfect MatchesListeria monocytogenesg+p+wgs
Resistomes with Sequence VariantsListeria monocytogenesg+p+wgs
Classification10 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic defensin [Antibiotic]
+ defensin resistant mprF [AMR Gene Family]
Publications

Thedieck K, et al. 2006. Mol. Microbiol. 62(5):1325-39 The MprF protein is required for lysinylation of phospholipids in listerial membranes and confers resistance to cationic antimicrobial peptides (CAMPs) on Listeria monocytogenes. (PMID 17042784)

Ernst CM, et al. 2009. PLoS Pathog 5(11): E1000660. The bacterial defensin resistance protein MprF consists of separable domains for lipid lysinylation and antimicrobial peptide repulsion. (PMID 19915718)

Resistomes

Prevalence of Listeria monocytogenes mprF among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Listeria monocytogenes98.32%0.97%73.93%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 1650


>gb|CAC99773.1|-|Listeria monocytogenes mprF [Listeria monocytogenes EGD-e]
MKEKLMQAYAWFQKNSTVVKIVFITFVMAFVIFEIINIATGIDYPSLKENLTSQSPEQIFIMFIVGLIAVTPMLLYDYVIVKLLPGKFSP
SHVIASGWITNTFTNIGGFGGVLGASLRASFYGKNASHKEILLAISKIALFLVSGLSIYCLVSLATLLIPGFADHFVNYWPWLLAGGLYF
PILFTITKWKSKSLFVDLPIKRELTLIIASLLEWGFAFGCFAIIGTLMGEPVDIFKVFPLFVIASVIGIASMVPGGVGTFDVVMILGLSQ
LGVSQELALAWMLFYRIFYYIIPFVVGLLFFVQKAGKKVNDFLEGLPLLFLQKVAHRFLVIFVYGSGLLLILSSAVPNAIYHVPFLYKIM
PFNFLFTSQITIVAFGFLLLGLARGIECKTKKAYIITVIVLGCAIFNTLARVFSMKQAIFLGIVLLCLFLARNEFYREKLVYTWSKVIID
SIIFIVCLAGYIVIGIYNSPNIKHSKEIPDYLRIASEHLWLVGFVGVFIAVVSLVIIYIYLSTTKEKLGSPFEAVKVREHLAKWGGNEVS
HTMFLRDKLLFWAAEGEVLFSYRIIADKMVIMGEPTGNMDKMEAAIEEVMMNADRFGYRPVFYEVRGTMIPYLHDHGFDFIKLGEEGFVD
VQNFTMSGKKKKGERALMNKLEREGYTFEIIEPPFNHDTWTTLRAVSDEWLDGREEKGFSLGFFDTYYLEQAPIAIAKNGEGTIVGFASM
MPSYTDEMTSIDLMRYSKEAPSGIMDFLFINLFEKAKEDGFQTFNAGMAPLANVGESKYAFLGERLAGLVYRYSQGFYGFKGLRNFKSKY
VTEWEQKFVAFRKRSSIAFTMLQLMILVGKKRPLANSQVVLDFPLEEETKKPDSE


>gb|AL591981.1|-|5694-8291|Listeria monocytogenes mprF [Listeria monocytogenes EGD-e]
ATGAAAGAAAAATTAATGCAAGCCTATGCCTGGTTTCAAAAAAATAGTACCGTCGTAAAAATCGTTTTTATTACTTTTGTGATGGCTTTT
GTTATTTTTGAAATTATTAATATTGCGACGGGGATTGACTATCCGTCGCTAAAAGAAAATTTAACTTCTCAAAGCCCGGAACAAATATTT
ATTATGTTTATCGTGGGCTTAATTGCTGTCACTCCAATGCTTTTGTATGATTATGTCATCGTTAAGTTGTTACCTGGAAAGTTTTCGCCA
AGTCATGTGATTGCCTCTGGTTGGATTACGAATACCTTTACTAATATTGGCGGTTTTGGTGGCGTATTAGGTGCCAGTTTAAGAGCAAGT
TTTTATGGGAAAAATGCATCTCATAAGGAAATTTTACTAGCTATTTCTAAGATTGCTTTATTTTTAGTATCTGGTTTATCGATTTACTGT
TTAGTATCATTAGCCACTTTACTCATTCCAGGATTTGCAGATCATTTTGTTAATTACTGGCCATGGCTTCTTGCGGGTGGTCTTTACTTC
CCGATTTTATTTACTATTACGAAATGGAAAAGTAAGTCACTCTTTGTTGATTTACCTATCAAAAGAGAATTAACGTTAATTATCGCTTCT
CTTTTGGAGTGGGGCTTCGCTTTTGGATGTTTCGCGATTATCGGTACATTGATGGGAGAACCAGTCGATATTTTCAAAGTGTTCCCGTTA
TTTGTTATTGCTTCGGTAATTGGGATTGCTTCGATGGTACCTGGTGGAGTAGGGACATTTGACGTCGTGATGATTCTTGGACTTAGCCAA
TTAGGTGTTTCTCAAGAATTAGCGCTCGCTTGGATGCTATTTTACCGAATTTTCTACTATATTATTCCTTTTGTAGTGGGACTACTTTTC
TTCGTCCAAAAAGCTGGTAAAAAAGTAAATGACTTTTTAGAAGGATTACCGTTATTATTCTTACAAAAAGTGGCCCATCGCTTCTTAGTT
ATTTTTGTTTACGGCTCTGGGTTATTGTTAATTTTGTCTTCCGCCGTACCAAACGCTATTTACCATGTGCCATTCTTATACAAAATTATG
CCGTTTAATTTCTTATTCACTTCCCAAATTACCATTGTTGCATTTGGCTTTTTACTACTGGGGCTTGCGAGAGGGATTGAATGTAAAACA
AAGAAAGCGTATATTATTACAGTAATTGTTCTAGGTTGCGCGATTTTCAACACACTTGCTCGCGTATTTTCGATGAAGCAGGCAATCTTT
TTAGGAATTGTGCTGTTATGTTTATTCTTAGCTCGAAACGAATTTTACCGAGAAAAACTGGTTTATACTTGGAGTAAAGTAATTATTGAT
AGCATTATTTTCATCGTATGTCTGGCAGGTTACATTGTTATCGGTATTTACAACTCACCAAATATCAAACACTCCAAAGAAATCCCTGAC
TATTTACGCATTGCCTCAGAGCATTTATGGTTAGTCGGCTTCGTTGGCGTATTTATCGCCGTTGTTAGTTTAGTCATTATTTACATTTAT
TTATCCACAACAAAAGAAAAACTTGGCTCTCCATTTGAAGCAGTCAAAGTACGCGAACATTTAGCGAAATGGGGCGGAAATGAAGTCAGT
CATACGATGTTCTTACGTGATAAACTGCTATTTTGGGCAGCAGAGGGGGAAGTACTTTTCTCTTACCGAATCATTGCGGACAAAATGGTC
ATCATGGGCGAACCAACTGGGAACATGGACAAAATGGAAGCAGCGATTGAAGAGGTAATGATGAACGCTGATAGATTTGGCTATCGACCT
GTTTTCTATGAAGTCCGGGGCACGATGATTCCATATTTACATGATCACGGATTTGACTTTATCAAGCTTGGCGAGGAAGGTTTTGTCGAC
GTCCAAAACTTTACAATGAGTGGTAAAAAGAAAAAAGGTGAGCGAGCTCTCATGAATAAATTAGAACGAGAAGGTTATACTTTTGAAATA
ATAGAACCACCATTCAATCACGACACTTGGACAACTTTACGAGCAGTTTCTGATGAGTGGCTAGATGGTAGGGAAGAAAAAGGTTTCTCA
TTAGGATTCTTCGATACGTATTATCTCGAACAAGCTCCGATTGCTATCGCTAAAAACGGAGAAGGTACTATCGTTGGATTTGCTTCGATG
ATGCCGTCATATACAGACGAAATGACTTCGATTGATTTAATGCGTTACTCCAAAGAAGCGCCATCAGGTATTATGGATTTCCTTTTCATT
AACCTATTCGAAAAAGCCAAAGAAGATGGCTTCCAAACATTTAATGCCGGTATGGCACCACTTGCCAATGTTGGGGAAAGTAAATATGCT
TTCCTAGGTGAACGATTAGCCGGACTTGTATACCGTTATAGTCAAGGTTTTTACGGTTTCAAAGGATTACGTAATTTTAAATCCAAATAT
GTTACAGAATGGGAACAAAAATTTGTTGCCTTTAGAAAAAGAAGTTCCATTGCTTTCACCATGTTACAATTAATGATTCTTGTTGGTAAA
AAACGACCACTTGCAAATAGCCAAGTAGTCCTTGATTTCCCACTCGAAGAAGAAACAAAAAAACCAGATTCTGAGTAA