Brucella suis mprF

Accession ARO:3003772
CARD Short NameBsui_mprF
DefinitionMprF is a integral membrane protein that modifies the negatively-charged phosphatidylglycerol on the membrane surface. This confers resistance to cationic peptides that disrupt the cell membrane, including defensins.
AMR Gene Familydefensin resistant mprF
Drug Classpeptide antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Perfect MatchesBrucella abortuswgs, Brucella melitensiswgs, Brucella suisg+wgs
Resistomes with Sequence VariantsBrucella abortusg+wgs, Brucella canisg+wgs, Brucella inopinatag+wgs, Brucella melitensisg+wgs, Brucella ovisg+wgs, Brucella suisg+wgs
Classification10 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic defensin [Antibiotic]
+ defensin resistant mprF [AMR Gene Family]
Publications

Ernst CM, et al. 2009. PLoS Pathog 5(11): E1000660. The bacterial defensin resistance protein MprF consists of separable domains for lipid lysinylation and antimicrobial peptide repulsion. (PMID 19915718)

Resistomes

Prevalence of Brucella suis mprF among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Brucella abortus76.98%0%66.5%0%
Brucella canis50%0%94.74%0%
Brucella inopinata50%0%100%0%
Brucella melitensis50.24%0%71.2%0%
Brucella ovis50%0%93.75%0%
Brucella suis50%0%60.42%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 1650


>gb|AAN33774.1|+|Brucella suis mprF [Brucella suis 1330]
MLWWDDISMSLIDDEIPSSQPQVSGRHFGWLKQYQHFLFPIAGIAIALLAIYVLENLLRHTSRTETLAALHNISWTTLALAVFFTALSYA
AVALYDVVAVDTIAPNQIPRRIAAVAGAAGYAISNALGFSLLTGGALRYRIYAAEGISLADIGKIVGTSWFAIWFALIIMVGAALLIDPQ
DVPFLSAIDIRIDIVAGILILGGIGWLIYWLSHGERNVSIGSFSLRLPNSKGALTQIFAGVVDVGAAAATLYVLLPEGAVPSFAVFALVY
VIAIVLGIASHAPGGLGAFEATIIAGLGLGGKPDAIAGLLAYRLIYTVLPLVVATAGILIWEVMRRRHMLDKQARFAKRLVEPLVPGLSA
SIIFLGGIILLISGATPDMRYRVKLLSDIVPEFLVEMSHLAASLVGVALLIVARGLSKRLERAWVAAMVLLLCGAVFSIAKGLDWEEASI
LCLFALSLWGFRDSFYRRPIAGPFELSWNWIATVGTTVLVSTWLGFFVYRHIEYSSDLWWDFAWNGNAPRFLRATVLVFAVVAAVGLHSI
INRHGQRRRKVDHSIPDAVPALVARCPHTDAALAMLGDKQFLLAPDDSAFIMYAQSGGSLIALGEPIGDAEAGKELAWSFHSLADRLALR
TVFYGVGPQSLPLFLDMGLIALKLGEVARVDLTDFSLEGPRRQPFRYADRKVDKDGLTFEIIPAADVPPLIPRLRAISDAWLDHKSGSEK
GFSLGYFNDEYLKRFDIAVLKKDGEIVAFANIWRGADKYEITVDLMRYMPNVHKLLMDALFAKLLTFSKQEGYKWFNLGAAPLSGLSGSR
LASRWNRFGSFIYRRGADLYHFDGLKAFKEKFDPVWTPHYMVCPGGLETPRALLDATTLINGSPLEFIRK


>gb|AE014292.2|+|568281-570923|Brucella suis mprF [Brucella suis 1330]
TTGCTCTGGTGGGACGACATTTCGATGAGCCTGATTGATGATGAAATACCTTCTTCGCAACCGCAAGTGTCAGGTAGGCATTTCGGCTGG
TTGAAACAATATCAGCATTTTCTGTTTCCAATCGCAGGCATCGCCATTGCGTTATTGGCTATTTATGTTCTGGAAAATCTTCTCAGGCAC
ACCTCCCGCACCGAAACGCTTGCAGCCCTTCACAATATTTCATGGACGACGCTCGCACTGGCCGTCTTTTTCACAGCATTAAGCTATGCC
GCAGTGGCGCTTTATGATGTCGTGGCGGTGGACACCATAGCGCCCAACCAGATACCGCGCCGCATCGCGGCGGTGGCGGGTGCTGCGGGC
TATGCGATTTCCAACGCGCTCGGCTTCTCGCTCCTGACCGGCGGGGCGCTGCGTTATCGCATTTATGCCGCAGAAGGCATAAGCCTGGCC
GATATCGGCAAGATCGTCGGCACCTCATGGTTCGCCATCTGGTTCGCCCTCATCATCATGGTGGGCGCAGCCCTGCTGATCGATCCGCAG
GACGTGCCTTTTCTTTCCGCCATCGATATACGGATTGATATTGTCGCAGGCATTCTCATCCTTGGCGGCATTGGCTGGCTGATCTACTGG
CTTTCGCATGGCGAACGCAATGTCAGCATCGGTTCGTTCAGCCTGCGCCTGCCCAATTCAAAAGGCGCGCTCACGCAGATTTTTGCGGGC
GTGGTGGATGTGGGCGCTGCCGCGGCCACGCTCTATGTGCTGTTGCCGGAAGGGGCTGTGCCAAGCTTCGCCGTCTTTGCGCTCGTTTAT
GTCATCGCCATCGTGCTTGGCATTGCAAGCCACGCGCCAGGCGGCCTCGGCGCTTTTGAGGCAACAATCATCGCAGGGCTTGGCCTCGGT
GGAAAACCCGATGCAATTGCGGGCCTGCTGGCCTATCGCCTCATTTACACGGTTCTTCCCCTCGTGGTTGCCACCGCCGGAATCCTCATC
TGGGAGGTCATGCGCCGCCGCCATATGCTCGACAAGCAGGCCCGATTCGCCAAGCGGCTGGTGGAGCCGCTGGTTCCGGGGCTTTCAGCC
AGCATCATCTTTCTCGGTGGCATCATCCTGCTTATCTCAGGCGCCACGCCCGATATGCGCTATCGTGTGAAGTTGCTGTCCGACATCGTG
CCGGAATTTCTTGTGGAGATGTCGCATCTGGCGGCAAGCCTCGTCGGCGTTGCTCTTCTCATCGTCGCGCGCGGCCTTTCCAAACGGCTG
GAGCGCGCCTGGGTGGCCGCGATGGTGCTTCTTCTTTGCGGCGCGGTGTTTTCGATCGCAAAAGGACTGGACTGGGAAGAGGCAAGCATT
TTGTGCCTTTTTGCCCTTTCACTCTGGGGCTTTCGCGATTCCTTCTACCGTCGTCCGATTGCCGGCCCGTTTGAACTGAGCTGGAACTGG
ATCGCAACCGTTGGAACCACGGTTCTCGTGTCCACCTGGCTTGGTTTTTTCGTCTACCGCCATATCGAATATTCAAGCGATCTCTGGTGG
GATTTCGCGTGGAACGGCAACGCCCCGCGTTTCCTGCGCGCCACCGTTCTGGTTTTCGCAGTCGTGGCGGCAGTCGGCCTTCATTCGATC
ATCAACCGGCATGGCCAGCGCAGGCGCAAGGTTGATCATTCCATCCCCGATGCCGTGCCCGCACTCGTCGCCCGGTGCCCGCATACAGAT
GCAGCCCTTGCCATGCTGGGCGACAAGCAATTTCTGCTGGCGCCCGACGACAGCGCGTTCATCATGTATGCGCAGTCGGGCGGCAGCCTG
ATCGCGCTTGGCGAGCCTATCGGCGATGCCGAAGCGGGCAAGGAACTTGCCTGGTCTTTCCATTCGCTTGCCGACCGTCTGGCGCTGCGC
ACTGTCTTCTATGGTGTCGGCCCGCAAAGCCTGCCGCTTTTCCTCGATATGGGCCTGATTGCGCTCAAGCTGGGTGAAGTCGCACGCGTT
GACCTGACAGATTTTTCGCTGGAAGGCCCGCGCCGCCAGCCCTTCCGTTATGCCGACCGCAAGGTGGACAAGGATGGGCTGACCTTCGAG
ATTATCCCTGCTGCGGACGTGCCGCCGCTGATCCCGCGCCTGCGCGCAATTTCCGACGCTTGGCTCGACCATAAATCAGGCAGCGAAAAG
GGTTTTTCGCTTGGCTATTTCAACGATGAGTATTTAAAGCGTTTCGATATTGCCGTGCTGAAGAAAGACGGCGAAATCGTTGCTTTCGCC
AATATATGGCGCGGTGCCGACAAGTATGAGATCACGGTCGACCTGATGCGCTACATGCCCAATGTGCACAAGCTCCTGATGGATGCGCTG
TTCGCCAAGCTGCTGACCTTTTCCAAGCAAGAAGGCTACAAATGGTTCAACCTTGGCGCGGCCCCGCTTTCCGGCCTTAGCGGCAGCAGG
CTTGCCTCGCGCTGGAACCGTTTCGGCTCGTTCATCTATCGCCGTGGGGCCGATCTTTATCACTTCGACGGGCTCAAAGCCTTCAAGGAA
AAATTCGATCCCGTCTGGACCCCGCATTATATGGTTTGCCCCGGCGGGCTTGAAACGCCGCGCGCCCTTCTTGATGCCACAACACTGATT
AACGGCAGCCCTTTGGAGTTCATTCGCAAATGA