Clostridium perfringens mprF

Accession ARO:3003773
CARD Short NameCper_mprF
DefinitionMprF is a integral membrane protein that modifies the negatively-charged phosphatidylglycerol on the membrane surface. This confers resistance to cationic peptides that disrupt the cell membrane, including defensins.
AMR Gene Familydefensin resistant mprF
Drug Classpeptide antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Perfect MatchesClostridium perfringensg+wgs
Resistomes with Sequence VariantsClostridium perfringensg+wgs
Classification10 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ defensin resistant mprF [AMR Gene Family]
+ confers_resistance_to_antibiotic defensin [Antibiotic]
Publications

Ernst CM, et al. 2009. PLoS Pathog 5(11): E1000660. The bacterial defensin resistance protein MprF consists of separable domains for lipid lysinylation and antimicrobial peptide repulsion. (PMID 19915718)

Resistomes

Prevalence of Clostridium perfringens mprF among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Clostridium perfringens97.5%0%48.41%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 1000


>gb|ABG86067.1|-|Clostridium perfringens mprF [Clostridium perfringens SM101]
MWDSLKKSYRHLKNILGFVTDKRNYENIKKLLKNYKILSDISNIIVSVLVFLSGILLIISGIYPSIFYKIKFLDNIYSLSFLRFSHRASI
LIGLMLIMTSKEVFFKVKRAYYVTLTLLIVGGAFAFIKDLDYKEGIFILGVIILLILSKKSFYRKSIPIKVTKLSGILIVLSIVMIIFAS
FIHKFNIHFSKNYKYYIDFFHSTKGYLRIALFTYISFIIFVIIWYLTMPKIEDDERYMDADLEKVSKFFKEIDYGTIFSHLVYLKDKKVF
WANEGESLIMYSKYKDKIIVLGDPIATKENLYSCIEEFQAFTNLYGYDVVFYEIEEKNFSTYHDAGYYFFKLGEEARIDLEEFNLIGSKK
SAFRNTLRRVEREGYNFSIIEPPFNNEVVSQLKEISDKWLGDRKEKGFSLGWFSEDYIQRSPIAILKNEEENKIMGFVTIMDANDGGETV
AIDLMRIDKDAPNASMDYLMLNLFLTFKEKGYKYFSLGEAPLSNVGFNTHSHLQEKLARLVYNSGNIFYSFDGLRRYKSKFSPIWQPRYL
AYPKFMSLPEVFINLCLLIANSKERVEKK


>gb|CP000312.1|-|1756065-1757774|Clostridium perfringens mprF [Clostridium perfringens SM101]
ATGTGGGATTCACTAAAAAAAAGTTATAGACATTTAAAAAATATTTTAGGATTTGTTACTGATAAAAGAAATTATGAAAATATAAAGAAG
CTATTAAAAAATTACAAAATCTTAAGTGATATATCAAATATAATAGTATCAGTTTTGGTATTTCTAAGTGGTATTCTTTTAATAATTTCA
GGGATTTATCCTAGTATATTTTATAAGATAAAATTTTTAGATAATATATACAGTTTATCTTTTTTAAGGTTTTCACATAGAGCTTCAATA
TTAATTGGATTAATGTTAATAATGACCTCTAAGGAAGTTTTCTTTAAGGTAAAAAGAGCTTATTATGTTACATTAACATTGCTTATAGTA
GGAGGAGCCTTTGCCTTTATAAAAGATTTAGATTACAAAGAAGGAATTTTTATTTTAGGAGTAATAATACTTCTAATATTATCAAAAAAG
AGTTTTTACAGAAAAAGTATTCCTATTAAGGTTACTAAATTAAGTGGGATATTAATAGTTCTTTCAATTGTAATGATTATCTTTGCGAGT
TTTATACATAAATTTAACATACATTTTAGCAAGAACTATAAATACTATATAGACTTTTTCCATAGCACAAAGGGGTATTTAAGAATAGCA
TTATTCACATATATATCCTTTATAATATTTGTGATAATATGGTATTTAACAATGCCTAAAATAGAAGATGACGAAAGGTATATGGATGCT
GATTTAGAAAAGGTATCAAAATTCTTTAAAGAAATAGATTATGGAACAATATTCTCCCATTTAGTTTATTTAAAGGATAAAAAGGTCTTT
TGGGCTAATGAAGGAGAGTCCTTAATAATGTATAGCAAGTACAAAGATAAGATAATAGTTTTAGGAGATCCTATAGCTACTAAGGAAAAC
CTATATAGTTGTATAGAAGAGTTTCAAGCTTTTACAAATTTATATGGATATGATGTTGTCTTTTATGAAATAGAAGAAAAAAACTTTTCT
ACCTATCATGATGCAGGGTATTATTTCTTTAAGTTAGGAGAAGAGGCAAGGATAGATTTAGAAGAATTTAATTTGATTGGTTCTAAAAAG
AGTGCCTTTAGAAACACCTTAAGAAGAGTTGAAAGGGAAGGATATAATTTTAGCATTATAGAGCCTCCTTTTAATAATGAGGTAGTAAGT
CAATTGAAGGAAATATCTGATAAATGGTTAGGGGACAGAAAAGAAAAGGGATTTTCTTTAGGATGGTTTAGTGAGGATTATATACAAAGA
TCACCTATAGCTATTTTAAAGAATGAAGAAGAAAATAAGATTATGGGCTTTGTAACAATAATGGATGCTAATGATGGAGGGGAGACAGTA
GCAATAGATTTAATGAGAATAGATAAAGATGCTCCAAATGCCTCTATGGATTACCTAATGCTTAATTTATTCTTAACCTTTAAAGAAAAA
GGATATAAGTATTTTAGCTTAGGAGAAGCACCATTATCTAATGTAGGATTTAACACTCATTCACATTTACAAGAAAAGCTTGCAAGGTTA
GTTTATAATAGTGGTAATATATTCTATAGTTTTGATGGACTAAGAAGATATAAGTCAAAGTTTTCTCCAATTTGGCAACCTAGATATTTA
GCATATCCTAAGTTTATGTCCTTACCAGAGGTGTTTATTAACTTATGTTTATTAATAGCTAATTCAAAGGAAAGAGTAGAGAAAAAATAA