| Accession | ARO:3003843 |
| CARD Short Name | leuO |
| Definition | leuO, a LysR family transcription factor, exists in a wide variety of bacteria of the family Enterobacteriaceae and is involved in the regulation of as yet unidentified genes affecting the stress response and pathogenesis expression. LeuO is also an activator of the MdtNOP efflux pump. |
| AMR Gene Family | major facilitator superfamily (MFS) antibiotic efflux pump |
| Drug Class | nucleoside antibiotic, disinfecting agents and antiseptics |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Efflux Regulator | protein(s) and two-component regulatory system modulating antibiotic efflux |
| Resistomes with Perfect Matches | Escherichia colig+wgs, Shigella flexnerig, Shigella sonneiwgs |
| Resistomes with Sequence Variants | Citrobacter amalonaticusg+wgs, Citrobacter freundiig+wgs, Citrobacter koserig+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniig+wgs, Citrobacter youngaeg+wgs, Enterobacter asburiaeg+wgs, Enterobacter cancerogenusg+wgs, Enterobacter chengduensisg+wgs, Enterobacter cloacaeg+wgs, Enterobacter hormaecheig+wgs, Enterobacter kobeig+wgs, Enterobacter roggenkampiig+wgs, Escherichia albertiig+wgs, Escherichia colig+p+wgs, Escherichia fergusoniig+wgs, Escherichia marmotaeg+wgs, Klebsiella aerogenesg+wgs, Klebsiella huaxiensisg+wgs, Klebsiella michiganensisg+wgs, Klebsiella oxytocag+wgs, Klebsiella pneumoniaeg+wgs, Klebsiella quasipneumoniaeg+wgs, Leclercia adecarboxylatag+wgs, Salmonella bongorig+wgs, Salmonella entericag+wgs, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs |
| Classification | 12 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + mechanism of antibiotic resistance + nucleoside antibiotic [Drug Class] + determinant of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + disinfecting agents and antiseptics [Drug Class] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + aminonucleoside antibiotic + major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family] + puromycin [Antibiotic] + acriflavine [Antibiotic] |
| Parent Term(s) | 2 ontology terms | Show + protein(s) and two-component regulatory system modulating antibiotic efflux [Efflux Regulator] + regulates MdtNOP |
| Publications | Shimada T, et al. 2009. J. Bacteriol. 191(14):4562-71 Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux. (PMID 19429622) Shimada T, et al. 2011. Mol. Microbiol. 82(2):378-97 Novel roles of LeuO in transcription regulation of E. coli genome: antagonistic interplay with the universal silencer H-NS. (PMID 21883529) |
Prevalence of leuO among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Citrobacter amalonaticus | 100% | 0% | 89.09% | 0% |
| Citrobacter freundii | 99.18% | 0% | 49.71% | 0% |
| Citrobacter koseri | 100% | 0% | 49.55% | 0% |
| Citrobacter portucalensis | 100% | 0% | 61.26% | 0% |
| Citrobacter werkmanii | 100% | 0% | 61.54% | 0% |
| Citrobacter youngae | 100% | 0% | 100% | 0% |
| Enterobacter asburiae | 93.55% | 0% | 69.17% | 0% |
| Enterobacter cancerogenus | 100% | 0% | 100% | 0% |
| Enterobacter chengduensis | 100% | 0% | 84% | 0% |
| Enterobacter cloacae | 96.43% | 0% | 71.88% | 0% |
| Enterobacter hormaechei | 98.56% | 0% | 65.82% | 0% |
| Enterobacter kobei | 90.91% | 0% | 63.76% | 0% |
| Enterobacter roggenkampii | 86.05% | 0% | 59.35% | 0% |
| Escherichia albertii | 100% | 0% | 62.58% | 0% |
| Escherichia coli | 66.63% | 0.01% | 61.19% | 0% |
| Escherichia fergusonii | 100% | 0% | 50% | 0% |
| Escherichia marmotae | 100% | 0% | 60.42% | 0% |
| Klebsiella aerogenes | 98% | 0% | 79.94% | 0% |
| Klebsiella huaxiensis | 100% | 0% | 66.67% | 0% |
| Klebsiella michiganensis | 96.77% | 0% | 69.15% | 0% |
| Klebsiella oxytoca | 100% | 0% | 73.53% | 0% |
| Klebsiella pneumoniae | 99.59% | 0% | 57.21% | 0% |
| Klebsiella quasipneumoniae | 100% | 0% | 73.16% | 0% |
| Leclercia adecarboxylata | 100% | 0% | 60.47% | 0% |
| Salmonella bongori | 100% | 0% | 94.74% | 0% |
| Salmonella enterica | 95.08% | 0% | 80.76% | 0% |
| Shigella boydii | 86.67% | 0% | 93.33% | 0% |
| Shigella dysenteriae | 21.43% | 0% | 30% | 0% |
| Shigella flexneri | 98% | 0% | 81.83% | 0% |
| Shigella sonnei | 100% | 0% | 92.99% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 500