| Accession | ARO:3004159 |
| CARD Short Name | Mgal_23S_PLM |
| Definition | Point mutations in the 23S rRNA (domain V) of Mycoplasma gallisepticum can confer resistance to pleuromutilin antibiotics (tiamulin and valnemulin). The mutants also show cross-resistance to lincomycin, chloramphenicol and florfenicol. |
| AMR Gene Family | 23S rRNA with mutation conferring resistance to pleuromutilin antibiotics |
| Drug Class | pleuromutilin antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target alteration [Resistance Mechanism] + mutation conferring antibiotic resistance + determinant of antibiotic resistance + antibiotic resistant gene variant or mutant + rRNA with mutation conferring antibiotic resistance + antibiotic molecule + pleuromutilin antibiotic [Drug Class] + 23S rRNA with mutation conferring antibiotic resistance |
| Parent Term(s) | 2 ontology terms | Show + confers_resistance_to_antibiotic pleuromutilin [Antibiotic] + 23S rRNA with mutation conferring resistance to pleuromutilin antibiotics [AMR Gene Family] |
| Publications | Li BB, et al. 2010. FEMS Microbiol. Lett. 308(2):144-9 Mutations in 23S rRNA gene associated with decreased susceptibility to tiamulin and valnemulin in Mycoplasma gallisepticum. (PMID 20487023) Long KS, et al. 2009. Mol. Microbiol. 71(5):1218-27 Single 23S rRNA mutations at the ribosomal peptidyl transferase centre confer resistance to valnemulin and other antibiotics in Mycobacterium smegmatis by perturbation of the drug binding pocket. (PMID 19154331) |
Prevalence of Mycoplasma gallisepticum 23S rRNA mutation conferring resistance to pleuromutilin antibiotics among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| No prevalence data | ||||
Model Type: rRNA gene variant model
Model Definition: Ribosomal RNA (rRNA) Gene Variant Models (RVM) are similar to Protein Variant Models (PVM), i.e. detect sequences based on their similarity to a curated reference sequence and secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles, except RVMs are designed to detect AMR acquired via mutation of genes encoding ribosomal RNAs (rRNA). RVMs include a rRNA reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTN bit-score above the curated BLASTN cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTN bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastN): 5000
Legend:
Published Variants:
| PMID: 20487023 | A2072G A2073G G2075T G2461A A2517T |
| PMID: 19154331 | C2069A G2461T T2518G A2586T |