Mycoplasma gallisepticum 23S rRNA mutation conferring resistance to pleuromutilin antibiotics

Download Sequences
Accession ARO:3004159
CARD Short NameMgal_23S_PLM
DefinitionPoint mutations in the 23S rRNA (domain V) of Mycoplasma gallisepticum can confer resistance to pleuromutilin antibiotics (tiamulin and valnemulin). The mutants also show cross-resistance to lincomycin, chloramphenicol and florfenicol.
AMR Gene Family23S rRNA with mutation conferring resistance to pleuromutilin antibiotics
Drug Classpleuromutilin antibiotic
Resistance Mechanismantibiotic target alteration
Classification10 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic pleuromutilin [Antibiotic]
+ 23S rRNA with mutation conferring resistance to pleuromutilin antibiotics [AMR Gene Family]
Publications

Li BB, et al. 2010. FEMS Microbiol. Lett. 308(2):144-9 Mutations in 23S rRNA gene associated with decreased susceptibility to tiamulin and valnemulin in Mycoplasma gallisepticum. (PMID 20487023)

Long KS, et al. 2009. Mol. Microbiol. 71(5):1218-27 Single 23S rRNA mutations at the ribosomal peptidyl transferase centre confer resistance to valnemulin and other antibiotics in Mycobacterium smegmatis by perturbation of the drug binding pocket. (PMID 19154331)
Resistomes

Prevalence of Mycoplasma gallisepticum 23S rRNA mutation conferring resistance to pleuromutilin antibiotics among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: rRNA gene variant model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GIGRDI-AMR2
No prevalence data


Detection Models

Model Type: rRNA gene variant model

Model Definition: Ribosomal RNA (rRNA) Gene Variant Models (RVM) are similar to Protein Variant Models (PVM), i.e. detect sequences based on their similarity to a curated reference sequence and secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles, except RVMs are designed to detect AMR acquired via mutation of genes encoding ribosomal RNAs (rRNA). RVMs include a rRNA reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTN bit-score above the curated BLASTN cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTN bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastN): 5000

PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).

MutationMutation typePubMed
c2069asingle resistance variantPMID:19154331
a2072gsingle resistance variantPMID:20487023
a2073gsingle resistance variantPMID:20487023
g2075tsingle resistance variantPMID:20487023
g2461tsingle resistance variantPMID:19154331
g2461asingle resistance variantPMID:20487023
a2517tsingle resistance variantPMID:20487023
t2518gsingle resistance variantPMID:19154331
a2586tsingle resistance variantPMID:19154331


>gb|NC_004829.2|+|80511-83423|Mycoplasma gallisepticum 23S rRNA mutation conferring resistance to pleuromutilin antibiotics [Mycoplasma gallisepticum str. R(low)]
ATACATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAGAAGGCGATGAAGGACGTGCAAACCTGCGAAATGCTACGGGGAGCTG
GTTGGAAGCGATAATCCGTAGATGTCCGAATGGGGGAACCTGATTAATAGTGATATTAATTATTTAGATCTGAATACATAGGGTCTAAAA
GCAATACGTTGTGAAGTGAAACATCTCAGTAGCAACAGGAAAAGAAATCGAAAGAGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAG
GCCAAACCAAGATTTATCTTGGGGTTATAGGACTGCAATGTGGACTTTGAACTGATAGGAGAAGTAGTTGAAAAGCTACGCGATAAAGGG
TTATAGCCCCGTATCTTAAATTGGTTTAATACCTAGCAGGATCCTGAGTACATCGAGAAACGTTATCTTGATGGAAGTCGCCCAGACCAT
TGGGCAAGCCTAAATACTAACTAGTGACCGATAGCGTATAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGGGATGGGAGTGAAATAGAT
TCTGAAGCCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAG
CAGGTTAACCTTTAGAAGGGAAGCCGAAGCGAAAGCGAGTTTGAATAGAGCGAATTAAAGTGTTTGTTATTATAGACCCGAAACGGGTTG
AGCTAGTCATGGGCAGGTTGAAGTTAGAGTAACATCTAATGGAGGACCGAACCGACTTTCGTTGAAACGACAGCGGATGACCTGTGACTA
GGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGTTTTAAGACTAGCGTAAGATCATGATCAACTGGAGGTAGA
GCTACTGAATGTATGATGGCGCCGCCTTGGTGTACTGAATACAATTAAACTCCGAATGCCAATTGATTTATTCTTGCAGTCAGACAGTGG
GGGATAAGCTTCATTGTCACAAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCTAAAATATGCTAAGTGGAAAAGGTTGTTAAAATACT
TAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAA
CGGGGCTAAGCATATTACCGAATTTATGGATTATTATTCGTAAGAATGATAGTGGTAGACGAGCGTTGTATATGGGATGAAGTCAAACCG
TGAGGATTGGTGGACTGTATACAAGTGAGAATGCCGGTGTAAGTAACGCTTGAGAGTGAGAATCTCTCAAACCGATTGACTAAGGTTTCC
TGGACGAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGGCGAGGCAGAAATACGTAGTCGATGGAAGAACAGGTTAATATTCCTGTAC
AAACAAATAGCTGATGGAGTGACGGAGAAGGTTAATGCATCCCCATTATCGGATTTGGGGTTAAATAAGAAGTCTTAAGGGTTGGCAAAT
CCGCCTTTTTTAAGGACAACTTATGAATACGAGTGAACGCTTTGCAAGTAGCGAAGATGCATACATCACGCTTCCAAGAAAAGCTTCTAG
GGTTAACTATTTGTTTCCAGTACCGAGAACGAACACACGTGGTCAAGGAGAAGATCCTAAGGTTAGCGAGTGAACTATAGCTAAGGAACT
CTGCAAATTCATCCCGTAAGTTCGCAAGAAGGGATGCTCAATGTAACAGTTGAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAA
ACACAGCTCTATGCTAAATCGCAAGATGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGGTGTTAGAGCAATCAA
AGCTCCCGACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAATTCCGTCCCGCT
TGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACG
GAAAGACCCCATGAAGCTTTACTGTAACTTAATATTGGGCAGAGTTTAGACATATAGAGAATAGGTGGGAGACTTTGAAGCAACTTCGCT
AGGAGTTGTGGAGTCACCAGTGGAATACCACCTTTGTTTAAATTCTTCTCTAACTAGTTGCTGTTATCCAGCAATAGGACAGTGTTAGGC
GGGCAGTTTGACTGGGGCGGTCGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTACCCTCAGCACGGTTGGAAATCGTGTTAAGAGTGT
AATGGTATAAGGGTGCTTGACTGTGAGACTAACAGGTCGAACAGGTAAGAAATTAGGTCATAGTGATCCGGTGGTTCAGTATGGAATGGC
CATCGCTCAACGGATAAAAGCTACTCTGGGGATAACAGGCTGATACTGCCCAAGAGTTCACATCGACGGCAGTGTTTGGCACCTCGATGT
CGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGC
GAGACAGGTTGGTCCCTATCTATTGTGCCCGCAGGAAGATTGAAAAGATTTACTCTTAGTACGAGAGGACCGGAGTGAAGACACCTCTTG
TGCTCCAGTTGTAGTGCCAACTGCACCGCTGGGTAGCAACGTGTCGAACGGATAAACGCTGAAAGCATCTAAGTGTGAAACCGACTTTAA
GAATAATCTTCCCTTCCAGCAATGGAGTAAGAATCGTTGTAGACTACGACGTTGATAGGCTAAAGGTGTAAGTGCCGCGAGGTATTTAGC
TGATTAGTACTAATAATTCGAGGACTTAGATTT

Curator Acknowledgements
Curator Description Most Recent Edit