NPS-1

Accession ARO:3004239
CARD Short NameNPS-1
DefinitionNPS-1 is a plasmid-encoded class D beta-lactamases identified from two Pseudomonas aeruginosa isolates in 1986.
AMR Gene FamilyNPS beta-lactamase
Drug Classpenicillin beta-lactam, cephalosporin
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesAchromobacter xylosoxidanswgs, Providencia rettgeriwgs, Pseudomonas aeruginosawgs, Pseudomonas stutzeriwgs
Resistomes with Sequence VariantsAchromobacter xylosoxidanswgs, Aeromonas caviaep+wgs, Aeromonas hydrophilap, Providencia rettgeriwgs, Pseudomonas aeruginosawgs, Pseudomonas stutzeriwgs
Classification13 ontology terms | Show
Parent Term(s)1 ontology terms | Show
+ NPS beta-lactamase [AMR Gene Family]
Publications

Livermore DM, et al. 1986. Antimicrob. Agents Chemother. 29(1):99-103 Characterization of NPS-1, a novel plasmid-mediated beta-lactamase, from two Pseudomonas aeruginosa isolates. (PMID 3089139)

Pai H and Jacoby GA. 2001. Antimicrob Agents Chemother 45(10): 2947-2948. Sequences of the NPS-1 and TLE-1 beta-lactamase genes. (PMID 11557499)

Resistomes

Prevalence of NPS-1 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GIGRDI-AMR2
Achromobacter xylosoxidans0%0%0.76%0%0%
Aeromonas caviae0%2.6%5.91%0%0%
Aeromonas hydrophila0%2.6%0%0%0%
Providencia rettgeri0%0%0.64%0%0%
Pseudomonas aeruginosa0%0%0.03%0%0%
Pseudomonas stutzeri0%0%0.76%0%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 450


>gb|AAK14791.1|+|NPS-1 [Pseudomonas aeruginosa]
MLKSTLLAFGLFIALSARAENQAIAQLFQRAGVDGTIVIESLTTRQRLVHNDPRAQQRYPAASTFKVLNTLIALEEGAISGENQIFHWNG
TQYSIANWNQDQTLDSAFKVSCVWCYQQIALRVGALKYPAYIQQTNYGHLLEPFNGTEFWLDGSLTISAEEQVAFLRRVVERKLPFKASS
YDSLKKVMFADENAQYRLYAKTGWATRITPSVGWYVGYVEAQDDVWLFALNLATRDANDLPLRTQIAKDALKAIGAFHAK


>gb|AY027589.1|+|46-828|NPS-1 [Pseudomonas aeruginosa]
ATGCTGAAGAGCACCCTTCTGGCCTTTGGTCTCTTTATCGCACTCTCAGCGCGTGCAGAGAACCAGGCAATCGCCCAGCTTTTCCAGAGG
GCAGGAGTCGATGGGACCATCGTCATCGAGTCTCTAACCACCAGACAGCGCTTGGTTCACAACGATCCTCGTGCGCAACAACGATACCCG
GCAGCTTCCACGTTCAAGGTACTCAATACCTTGATTGCTCTCGAAGAGGGCGCCATTTCAGGTGAGAACCAGATCTTTCACTGGAACGGT
ACCCAGTATTCGATTGCGAATTGGAACCAGGACCAGACTCTAGACAGTGCGTTTAAAGTGAGTTGTGTCTGGTGCTACCAGCAGATTGCC
CTTCGAGTGGGGGCACTCAAGTACCCAGCCTATATTCAACAGACAAACTATGGTCATTTACTGGAACCCTTCAATGGAACGGAGTTTTGG
CTGGATGGCTCTTTGACGATCAGCGCGGAAGAACAGGTTGCCTTTCTCCGACGGGTTGTTGAGCGAAAACTACCGTTCAAAGCGAGCAGC
TATGATTCCCTGAAGAAAGTCATGTTCGCCGATGAGAATGCCCAGTATCGCCTTTATGCAAAAACGGGTTGGGCGACCCGCATCACTCCC
TCGGTGGGTTGGTATGTTGGCTATGTTGAAGCACAGGACGATGTTTGGCTGTTTGCCCTGAATCTTGCTACCCGCGACGCAAATGACCTG
CCCCTACGAACGCAGATAGCCAAAGACGCGCTGAAGGCGATAGGTGCGTTTCATGCGAAGTAA

Curator Acknowledgements
Curator Description Most Recent Edit