| Accession | ARO:3004550 |
| CARD Short Name | dfrA27 |
| Definition | A dihydrofolate reductase and trimethoprim resistance gene from non-O1, non-O139 Vibrio cholerae. |
| AMR Gene Family | trimethoprim resistant dihydrofolate reductase dfr |
| Drug Class | diaminopyrimidine antibiotic |
| Resistance Mechanism | antibiotic target replacement |
| Resistomes with Perfect Matches | Acinetobacter baumanniig+wgs, Citrobacter freundiip+wgs, Citrobacter portucalensiswgs, Citrobacter werkmaniiwgs, Citrobacter youngaewgs, Comamonas testosteroniwgs, Enterobacter asburiaewgs, Enterobacter hormaecheip+wgs, Enterobacter kobeiwgs, Enterobacter roggenkampiiwgs, Escherichia colig+p+wgs, Escherichia fergusoniiwgs, Klebsiella aerogeneswgs, Klebsiella michiganensisp+wgs, Klebsiella oxytocawgs, Klebsiella pneumoniaep+wgs, Klebsiella quasipneumoniaep+wgs, Laribacter hongkongensiswgs, Leclercia adecarboxylatap+wgs, Morganella morganiig+wgs, Proteus mirabilisg+wgs, Providencia alcalifaciensg, Providencia rettgerig+wgs, Pseudomonas aeruginosap+wgs, Raoultella planticolap+wgs, Salmonella entericag+p+wgs, Serratia marcescenswgs, Shigella flexnerip+wgs, Vibrio alginolyticusg, Vibrio choleraep, Vibrio harveyiwgs, Vibrio parahaemolyticusp+wgs |
| Resistomes with Sequence Variants | Acinetobacter baumanniig+wgs, Acinetobacter nosocomialiswgs, Aeromonas veroniig, Citrobacter freundiip+wgs, Citrobacter koseriwgs, Citrobacter portucalensisp+wgs, Citrobacter werkmaniip+wgs, Citrobacter youngaewgs, Comamonas testosteroniwgs, Enterobacter asburiaewgs, Enterobacter cloacaewgs, Enterobacter hormaecheip+wgs, Enterobacter kobeiwgs, Enterobacter roggenkampiip+wgs, Escherichia colig+p+wgs, Escherichia fergusoniiwgs, Klebsiella aerogenesp+wgs, Klebsiella huaxiensisg, Klebsiella michiganensisp+wgs, Klebsiella oxytocawgs, Klebsiella pneumoniaep+wgs, Klebsiella quasipneumoniaep+wgs, Laribacter hongkongensiswgs, Leclercia adecarboxylatap+wgs, Morganella morganiig+wgs, Proteus mirabilisg+wgs, Providencia alcalifaciensg, Providencia rettgerig+wgs, Pseudomonas aeruginosap+wgs, Raoultella planticolap+wgs, Salmonella entericag+p+wgs, Serratia marcescenswgs, Shigella flexnerip+wgs, Vibrio alginolyticusg+wgs, Vibrio choleraep, Vibrio harveyiwgs, Vibrio parahaemolyticusp+wgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic target replacement [Resistance Mechanism] + antibiotic target replacement protein + diaminopyrimidine antibiotic [Drug Class] + antibiotic resistant dihydrofolate reductase + trimethoprim [Antibiotic] |
| Parent Term(s) | 2 ontology terms | Show + confers_resistance_to_antibiotic trimethoprim [Antibiotic] + trimethoprim resistant dihydrofolate reductase dfr [AMR Gene Family] |
| Publications | Wu J, et al. 2015. Antimicrob. Agents Chemother. 59(7):4293-5 Molecular characterization of ISCR1-mediated blaPER-1 in a non-O1, non-O139 Vibrio cholerae strain from China. (PMID 25870070) |
Prevalence of dfrA27 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| Acinetobacter baumannii | 0.18% | 0% | 0.06% | 0% | 0% |
| Acinetobacter nosocomialis | 0% | 0% | 0.57% | 0% | 0% |
| Aeromonas veronii | 1.82% | 0% | 0% | 0% | 0% |
| Citrobacter freundii | 0% | 0.92% | 5.61% | 0% | 0% |
| Citrobacter koseri | 0% | 0% | 0.9% | 0% | 0% |
| Citrobacter portucalensis | 0% | 1.47% | 1.8% | 0% | 0% |
| Citrobacter werkmanii | 0% | 10% | 2.56% | 0% | 0% |
| Citrobacter youngae | 0% | 0% | 6.25% | 0% | 0% |
| Comamonas testosteroni | 0% | 0% | 7.14% | 0% | 0% |
| Enterobacter asburiae | 0% | 0% | 1.58% | 0% | 0% |
| Enterobacter cloacae | 0% | 0% | 0.32% | 0% | 0% |
| Enterobacter hormaechei | 0% | 0.51% | 3.11% | 0% | 0% |
| Enterobacter kobei | 0% | 0% | 3.06% | 0% | 0% |
| Enterobacter roggenkampii | 0% | 0.48% | 2.16% | 0% | 0% |
| Escherichia coli | 0.05% | 0.21% | 0.65% | 0% | 0% |
| Escherichia fergusonii | 0% | 0% | 1.09% | 0% | 0% |
| Klebsiella aerogenes | 0% | 1.09% | 1.13% | 0% | 0% |
| Klebsiella huaxiensis | 100% | 0% | 0% | 0% | 0% |
| Klebsiella michiganensis | 0% | 2.29% | 2.66% | 0% | 0% |
| Klebsiella oxytoca | 0% | 0% | 0.84% | 0% | 0% |
| Klebsiella pneumoniae | 0% | 1.54% | 4.96% | 0% | 0% |
| Klebsiella quasipneumoniae | 0% | 2.12% | 5.79% | 0% | 0% |
| Laribacter hongkongensis | 0% | 0% | 4.17% | 0% | 0% |
| Leclercia adecarboxylata | 0% | 4.76% | 4.65% | 0% | 0% |
| Morganella morganii | 3.85% | 0% | 1.84% | 0% | 0% |
| Proteus mirabilis | 2.75% | 0% | 0.66% | 0% | 0% |
| Providencia alcalifaciens | 9.09% | 0% | 0% | 0% | 0% |
| Providencia rettgeri | 5.88% | 0% | 0.64% | 0% | 0% |
| Pseudomonas aeruginosa | 0% | 1.46% | 0.16% | 0% | 0% |
| Raoultella planticola | 0% | 6.98% | 2.56% | 0% | 0% |
| Salmonella enterica | 0.19% | 0.33% | 0.35% | 0% | 0% |
| Serratia marcescens | 0% | 0% | 0.79% | 0% | 0% |
| Shigella flexneri | 0% | 1.2% | 0.31% | 0% | 0% |
| Vibrio alginolyticus | 1.23% | 0% | 0.79% | 0% | 0% |
| Vibrio cholerae | 0% | 5.26% | 0% | 0% | 0% |
| Vibrio harveyi | 0% | 0% | 4% | 0% | 0% |
| Vibrio parahaemolyticus | 0% | 0.62% | 0.05% | 0% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 300
| Curator | Description | Most Recent Edit |
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