dfrA18

Accession ARO:3004568
Synonym(s)dfr18
DefinitionA trimethoprim-resistant dihydrofolate reductase identified from Vibrio cholerae
AMR Gene Familytrimethoprim resistant dihydrofolate reductase dfr
Drug Classdiaminopyrimidine antibiotic
Resistance Mechanismantibiotic target replacement
ResistomesVibrio choleraewgs
Classification10 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic trimethoprim [Antibiotic]
+ trimethoprim resistant dihydrofolate reductase dfr [AMR Gene Family]
Publications

Hochhut B, et al. 2001. Antimicrob Agents Chemother 45(11): 2991-3000. Molecular analysis of antibiotic resistance gene clusters in vibrio cholerae O139 and O1 SXT constins. (PMID 11600347)

Resistomes

Prevalence of dfrA18 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
Vibrio cholerae0%0%1.08%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 300


>gb|AAK64581.1|-|dfrA18 [Vibrio cholerae MO10]
MNKEIQFSMIVARGVNGEIGQDGDLPWHVEGVRLKEDLKRFKAITMGKTLVMGRKTFESIPNGLPGRNVIVLTREPYDKADITERGDGTF
VAWGNSCHLFEVAEHLGVTEIIVAGGAEIYNLHKDVITKVFETKVLRAYPAADTHVDVFWESPGYDTEGRQWRVTSRGHIIENGSFTIAT
TYER


>gb|AY034138.1|-|7413-7967|dfrA18 [Vibrio cholerae MO10]
ATGAATAAAGAAATTCAATTTTCAATGATTGTAGCGCGTGGTGTAAACGGTGAGATTGGTCAGGACGGCGACTTGCCTTGGCATGTCGAA
GGAGTTCGGTTGAAAGAAGACCTCAAACGCTTCAAAGCGATCACTATGGGTAAGACACTCGTCATGGGCAGAAAGACCTTTGAGAGCATC
CCCAATGGCCTGCCCGGACGCAACGTCATCGTGCTCACACGCGAGCCTTACGATAAAGCGGATATAACCGAACGTGGAGACGGCACCTTT
GTAGCGTGGGGGAATAGCTGCCATCTGTTTGAAGTGGCCGAACACCTGGGCGTTACTGAGATCATCGTGGCGGGCGGTGCTGAAATCTAT
AATCTGCACAAAGACGTCATTACGAAGGTTTTTGAAACGAAGGTGTTGAGGGCGTATCCTGCAGCGGATACTCACGTAGACGTATTCTGG
GAAAGTCCCGGATACGACACTGAAGGCCGACAATGGCGCGTAACGTCCAGAGGCCACATTATCGAAAACGGCAGTTTCACTATCGCGACG
ACGTATGAGCGATAG