Accession ARO:3005350
Synonym(s)trimethoprim-resistant dihydrofolate reductase DfrB9
CARD Short NameDfrB9
DefinitionDfrB9 is a dihydrofolate reductase that confers resistant to Trimethoprim. Detected directly from NCBI and submitted without publication.
AMR Gene Familytrimethoprim resistant dihydrofolate reductase dfr
Drug Classdiaminopyrimidine antibiotic
Resistance Mechanismantibiotic target replacement
Resistomes with Perfect MatchesEnterobacter hormaecheip, Klebsiella pneumoniaewgs
Resistomes with Sequence VariantsAcinetobacter baumanniig, Aeromonas caviaewgs, Aeromonas veroniiwgs, Comamonas testosteronip, Enterobacter hormaecheip, Klebsiella pneumoniaep+wgs, Pseudomonas aeruginosap+wgs, Pseudomonas putidap
Classification9 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ trimethoprim resistant dihydrofolate reductase dfr [AMR Gene Family]
+ confers_resistance_to_antibiotic trimethoprim [Antibiotic]

Toulouse JL, et al. 2020. ACS Med Chem Lett 11(11):2261-2267 Dual-Target Inhibitors of the Folate Pathway Inhibit Intrinsically Trimethoprim-Resistant DfrB Dihydrofolate Reductases. (PMID 33214838)

Lemay-St-Denis C, et al. 2021. Antibiotics (Basel) 10(4): The Bacterial Genomic Context of Highly Trimethoprim-Resistant DfrB Dihydrofolate Reductases Highlights an Emerging Threat to Public Health. (PMID 33924456)


Prevalence of DfrB9 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Acinetobacter baumannii0.18%0%0%0%
Aeromonas caviae0%0%6.45%0%
Aeromonas veronii0%0%1.69%0%
Comamonas testosteroni0%50%0%0%
Enterobacter hormaechei0%0.06%0%0%
Klebsiella pneumoniae0%0.02%0.02%0%
Pseudomonas aeruginosa0%0.29%0.13%0%
Pseudomonas putida0%4%0%0%
Show Perfect Only

Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 125

>gb|WP_071846200.1|+|DfrB9 [Enterobacter cloacae]

>gb|NG_052167.1|+|101-337|DfrB9 [Enterobacter cloacae]