| Accession | ARO:3000777 |
| CARD Short Name | adeF |
| Definition | AdeF is the membrane fusion protein of the multidrug efflux complex AdeFGH. |
| AMR Gene Family | resistance-nodulation-cell division (RND) antibiotic efflux pump |
| Drug Class | tetracycline antibiotic, fluoroquinolone antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Perfect Matches | Acinetobacter baumanniig+wgs |
| Resistomes with Sequence Variants | Achromobacter insolitusg+wgs, Achromobacter xylosoxidansg+wgs+gi, Acinetobacter baumanniig+p+wgs, Acinetobacter calcoaceticusg+wgs, Acinetobacter haemolyticusg+wgs+gi, Acinetobacter johnsoniig+p+wgs+gi, Acinetobacter juniig+wgs, Acinetobacter lwoffiiwgs, Acinetobacter nosocomialisg+p+wgs, Acinetobacter pittiig+p+wgs, Aeromonas caviaeg+p+wgs, Aeromonas enteropelogenesg+wgs, Aeromonas hydrophilag+wgs, Aeromonas veroniig+wgs+gi, Alcaligenes faecalisg+wgs, Aliarcobacter butzlerig+wgs, Alistipes putrediniswgs, Bacteroides caccaeg+wgs, Bacteroides fragilisg+wgs, Bacteroides ovatusg+wgs, Bacteroides thetaiotaomicrong+wgs, Bordetella bronchisepticag+wgs, Bordetella hinziig+wgs, Bordetella holmesiig+wgs, Bordetella parapertussisg+wgs, Bordetella pertussisg+wgs, Bordetella petriig+wgs, Bordetella trematumg+wgs, Brucella abortusg+wgs, Brucella canisg+wgs, Brucella inopinatag+wgs, Brucella intermediag+wgs, Brucella melitensisg+wgs, Brucella ovisg+wgs, Brucella suisg+wgs, Burkholderia ambifariag+wgs, Burkholderia cenocepaciag+p+wgs+gi, Burkholderia cepaciag+wgs, Burkholderia contaminansg+wgs+gi, Burkholderia dolosag+wgs, Burkholderia glumaeg+wgs, Burkholderia latag+wgs+gi, Burkholderia malleig+wgs, Burkholderia multivoransg+wgs, Burkholderia oklahomensisg+wgs, Burkholderia pseudomalleig+wgs, Burkholderia thailandensisg+wgs, Burkholderia vietnamiensisg+p+wgs, Butyricimonas faecalisg, Campylobacter concisusg+wgs, Campylobacter fetuswgs, Campylobacter rectusg+wgs, Campylobacter showaeg+wgs, Chryseobacterium nakagawaig+wgs+gi, Citrobacter amalonaticusg+wgs, Citrobacter freundiig+wgs+gi, Citrobacter koserig+wgs, Citrobacter portucalensisg+wgs, Citrobacter youngaewgs, Comamonas testosteronig+wgs, Cronobacter condimentig+wgs, Cronobacter dublinensisg+wgs, Cronobacter malonaticusg+wgs, Cronobacter sakazakiig+wgs+gi, Cronobacter turicensiswgs, Cronobacter universalisg+wgs, Cupriavidus campinensisg+p+wgs+gi, Cupriavidus metalliduransg+p+wgs+gi, Cytophaga hutchinsoniig+wgs+gi, Dechloromonas aromaticagi, Delftia tsuruhatensisg+wgs, Edwardsiella tardag+wgs, Elizabethkingia anophelisg+wgs, Elizabethkingia miricolag+wgs, Enterobacter asburiaeg+wgs+gi, Enterobacter cancerogenusg+wgs, Enterobacter chengduensisg+wgs+gi, Enterobacter cloacaeg+p+wgs+gi, Enterobacter hormaecheig+p+wgs+gi, Enterobacter kobeig+wgs+gi, Enterobacter roggenkampiig+wgs, Escherichia albertiip+wgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniip+wgs, Granulibacter bethesdensisg, Helicobacter canadensisg+wgs, Helicobacter cinaedig+wgs, Helicobacter pullorumg+wgs, Inquilinus limosuswgs, Klebsiella aerogenesg+p+wgs, Klebsiella huaxiensisg+wgs+gi, Klebsiella michiganensisg+p+wgs, Klebsiella oxytocag+wgs, Klebsiella pneumoniaeg+p+wgs+gi, Klebsiella quasipneumoniaeg+wgs+gi, Kosakonia arachidisg+wgs+gi, Laribacter hongkongensisg+wgs, Leclercia adecarboxylatag+wgs+gi, Legionella anisag+wgs, Legionella longbeachaeg+wgs, Legionella pneumophilag+wgs, Luteibacter pinisolig+gi, Methylorubrum populig+wgs+gi, Moraxella osloensiswgs, Nitrosomonas europaeag+wgs+gi, Orrella dioscoreaeg+gi, Pannonibacter phragmitetuswgs, Parabacteroides distasonisg+wgs, Paracoccus kondratievaeg+gi, Phocaeicola doreig+wgs, Phocaeicola massiliensiswgs, Phocaeicola vulgatusg+wgs, Photobacterium profundumg+wgs+gi, Plesiomonas shigelloidesg+p+wgs, Proteus mirabilisg+wgs, Proteus penneriwgs, Proteus vulgarisg+wgs, Providencia alcalifaciensg, Providencia heimbachaeg+wgs, Providencia rettgerig+p+wgs, Providencia stuartiig+wgs, Pseudomonas aeruginosag+p+wgs+gi, Pseudomonas brassicacearumg+wgs+gi, Pseudomonas chlororaphisg+wgs+gi, Pseudomonas fluorescensg+wgs+gi, Pseudomonas koreensisg+wgs+gi, Pseudomonas mendocinag+wgs, Pseudomonas monteiliig+wgs, Pseudomonas putidag+wgs+gi, Pseudomonas stutzerig+wgs, Pseudomonas synxanthag+wgs+gi, Pseudomonas syringaeg+wgs, Ralstonia mannitolilyticag+wgs, Ralstonia pickettiig+wgs, Ralstonia solanacearumg+p+wgs, Raoultella planticolag+wgs, Rhizobium leguminosarumg+p+wgs+gi, Rhodanobacter glycinisg+wgs+gi, Rhodopseudomonas palustrisg+wgs+gi, Salmonella bongorig+wgs, Salmonella entericag+p+wgs+gi, Serratia liquefaciensg+wgs, Serratia marcescensg+p+wgs, Serratia odoriferag+wgs, Serratia rubidaeag+wgs+gi, Shewanella putrefaciensg+wgs, Sphingobacterium hotanenseg+wgs, Sphingobium yanoikuyaeg+p+wgs, Stenotrophomonas maltophiliag+wgs+gi, Thalassospira indicag+gi, Vibrio alginolyticusg+wgs, Vibrio fluvialisg+wgs, Vibrio harveyig+wgs, Vibrio neocaledonicusg, Vibrio owensiig+wgs, Vibrio parahaemolyticusg+wgs, Vibrio vulnificusg+wgs, Xanthomonas campestrisg+wgs, Yersinia canariaeg+wgs, Yersinia enterocoliticag+wgs, Yersinia kristenseniig+wgs, Yersinia pestisg+wgs, Yersinia pseudotuberculosisg+wgs |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic efflux [Resistance Mechanism] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + tetracycline antibiotic [Drug Class] + resistance-nodulation-cell division (RND) antibiotic efflux pump [AMR Gene Family] + tetracycline [Antibiotic] + fluoroquinolone antibiotic [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Coyne S, et al. 2010. Antimicrob Agents Chemother 54(10): 4389-4393. Overexpression of resistance-nodulation-cell division pump AdeFGH confers multidrug resistance in Acinetobacter baumannii. (PMID 20696879) |
Prevalence of adeF among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Achromobacter insolitus | 100% | 0% | 85.71% | 0% |
| Achromobacter xylosoxidans | 100% | 0% | 55.73% | 14.29% |
| Acinetobacter baumannii | 98.94% | 0.52% | 64.49% | 0% |
| Acinetobacter calcoaceticus | 100% | 0% | 82.61% | 0% |
| Acinetobacter haemolyticus | 85.71% | 0% | 65.12% | 88.89% |
| Acinetobacter johnsonii | 5.26% | 2.78% | 21.82% | 33.33% |
| Acinetobacter junii | 18.18% | 0% | 11.94% | 0% |
| Acinetobacter lwoffii | 0% | 0% | 7.89% | 0% |
| Acinetobacter nosocomialis | 100% | 1.64% | 73.56% | 0% |
| Acinetobacter pittii | 100% | 0.99% | 76.7% | 0% |
| Aeromonas caviae | 97.73% | 1.3% | 76.88% | 0% |
| Aeromonas enteropelogenes | 100% | 0% | 100% | 0% |
| Aeromonas hydrophila | 100% | 0% | 80.65% | 0% |
| Aeromonas veronii | 94.55% | 0% | 65.17% | 12.5% |
| Alcaligenes faecalis | 90% | 0% | 82.35% | 0% |
| Aliarcobacter butzleri | 100% | 0% | 25.2% | 0% |
| Alistipes putredinis | 0% | 0% | 88% | 0% |
| Bacteroides caccae | 100% | 0% | 95% | 0% |
| Bacteroides fragilis | 100% | 0% | 55.17% | 0% |
| Bacteroides ovatus | 100% | 0% | 44.87% | 0% |
| Bacteroides thetaiotaomicron | 100% | 0% | 42.66% | 0% |
| Bordetella bronchiseptica | 100% | 0% | 97.26% | 0% |
| Bordetella hinzii | 100% | 0% | 81.82% | 0% |
| Bordetella holmesii | 100% | 0% | 64.29% | 0% |
| Bordetella parapertussis | 100% | 0% | 100% | 0% |
| Bordetella pertussis | 95.28% | 0% | 90.88% | 0% |
| Bordetella petrii | 100% | 0% | 83.33% | 0% |
| Bordetella trematum | 100% | 0% | 100% | 0% |
| Brucella abortus | 52.38% | 0% | 71% | 0% |
| Brucella canis | 100% | 0% | 94.74% | 0% |
| Brucella inopinata | 100% | 0% | 100% | 0% |
| Brucella intermedia | 100% | 0% | 75.68% | 0% |
| Brucella melitensis | 95.73% | 0% | 71.84% | 0% |
| Brucella ovis | 50% | 0% | 93.75% | 0% |
| Brucella suis | 95.83% | 0% | 60.42% | 0% |
| Burkholderia ambifaria | 39.53% | 0% | 23.38% | 0% |
| Burkholderia cenocepacia | 87.44% | 3.33% | 69.96% | 50% |
| Burkholderia cepacia | 89.09% | 0% | 79.82% | 0% |
| Burkholderia contaminans | 91.84% | 0% | 53.75% | 60% |
| Burkholderia dolosa | 66.67% | 0% | 50% | 0% |
| Burkholderia glumae | 100% | 0% | 62.5% | 0% |
| Burkholderia lata | 77.78% | 0% | 37.5% | 100% |
| Burkholderia mallei | 93.22% | 0% | 55.95% | 0% |
| Burkholderia multivorans | 67.95% | 0% | 50.96% | 0% |
| Burkholderia oklahomensis | 70% | 0% | 100% | 0% |
| Burkholderia pseudomallei | 97.25% | 0% | 86.27% | 0% |
| Burkholderia thailandensis | 97.83% | 0% | 73.58% | 0% |
| Burkholderia vietnamiensis | 65.62% | 9.09% | 52.59% | 0% |
| Butyricimonas faecalis | 100% | 0% | 0% | 0% |
| Campylobacter concisus | 50% | 0% | 42.73% | 0% |
| Campylobacter fetus | 0% | 0% | 1.33% | 0% |
| Campylobacter rectus | 100% | 0% | 100% | 0% |
| Campylobacter showae | 100% | 0% | 100% | 0% |
| Chryseobacterium nakagawai | 100% | 0% | 100% | 100% |
| Citrobacter amalonaticus | 90.91% | 0% | 81.82% | 0% |
| Citrobacter freundii | 5.74% | 0% | 15.09% | 9.09% |
| Citrobacter koseri | 100% | 0% | 49.55% | 0% |
| Citrobacter portucalensis | 3.7% | 0% | 3.6% | 0% |
| Citrobacter youngae | 0% | 0% | 6.25% | 0% |
| Comamonas testosteroni | 100% | 0% | 92.86% | 0% |
| Cronobacter condimenti | 100% | 0% | 100% | 0% |
| Cronobacter dublinensis | 100% | 0% | 100% | 0% |
| Cronobacter malonaticus | 100% | 0% | 87.27% | 0% |
| Cronobacter sakazakii | 100% | 0% | 91.93% | 50% |
| Cronobacter turicensis | 0% | 0% | 83.33% | 0% |
| Cronobacter universalis | 100% | 0% | 100% | 0% |
| Cupriavidus campinensis | 100% | 50% | 100% | 100% |
| Cupriavidus metallidurans | 100% | 16.67% | 100% | 100% |
| Cytophaga hutchinsonii | 100% | 0% | 100% | 100% |
| Dechloromonas aromatica | 0% | 0% | 0% | 100% |
| Delftia tsuruhatensis | 100% | 0% | 100% | 0% |
| Edwardsiella tarda | 100% | 0% | 93.33% | 0% |
| Elizabethkingia anophelis | 100% | 0% | 67.01% | 0% |
| Elizabethkingia miricola | 100% | 0% | 82.61% | 0% |
| Enterobacter asburiae | 100% | 0% | 71.15% | 33.33% |
| Enterobacter cancerogenus | 100% | 0% | 100% | 0% |
| Enterobacter chengduensis | 100% | 0% | 84% | 50% |
| Enterobacter cloacae | 100% | 0.56% | 73.48% | 10% |
| Enterobacter hormaechei | 99.64% | 0.06% | 67.2% | 10% |
| Enterobacter kobei | 100% | 0% | 68.56% | 50% |
| Enterobacter roggenkampii | 97.67% | 0% | 62.23% | 0% |
| Escherichia albertii | 0% | 0.56% | 1.29% | 0% |
| Escherichia coli | 0.02% | 0.26% | 1.02% | 0.13% |
| Escherichia fergusonii | 0% | 1.07% | 3.26% | 0% |
| Granulibacter bethesdensis | 100% | 0% | 0% | 0% |
| Helicobacter canadensis | 100% | 0% | 100% | 0% |
| Helicobacter cinaedi | 100% | 0% | 94.12% | 0% |
| Helicobacter pullorum | 100% | 0% | 93.1% | 0% |
| Inquilinus limosus | 0% | 0% | 75% | 0% |
| Klebsiella aerogenes | 100% | 1.09% | 79.94% | 0% |
| Klebsiella huaxiensis | 100% | 0% | 66.67% | 50% |
| Klebsiella michiganensis | 100% | 0.57% | 69.15% | 0% |
| Klebsiella oxytoca | 100% | 0% | 74.79% | 0% |
| Klebsiella pneumoniae | 98.93% | 0.22% | 56.94% | 2.86% |
| Klebsiella quasipneumoniae | 100% | 0% | 73.68% | 100% |
| Kosakonia arachidis | 100% | 0% | 100% | 100% |
| Laribacter hongkongensis | 100% | 0% | 10.42% | 0% |
| Leclercia adecarboxylata | 100% | 0% | 55.81% | 50% |
| Legionella anisa | 100% | 0% | 100% | 0% |
| Legionella longbeachae | 100% | 0% | 100% | 0% |
| Legionella pneumophila | 100% | 0% | 80.52% | 0% |
| Luteibacter pinisoli | 100% | 0% | 0% | 100% |
| Methylorubrum populi | 100% | 0% | 100% | 100% |
| Moraxella osloensis | 0% | 0% | 7.14% | 0% |
| Nitrosomonas europaea | 100% | 0% | 100% | 100% |
| Orrella dioscoreae | 100% | 0% | 0% | 100% |
| Pannonibacter phragmitetus | 0% | 0% | 16.67% | 0% |
| Parabacteroides distasonis | 100% | 0% | 66.36% | 0% |
| Paracoccus kondratievae | 50% | 0% | 0% | 100% |
| Phocaeicola dorei | 100% | 0% | 79.17% | 0% |
| Phocaeicola massiliensis | 0% | 0% | 96.15% | 0% |
| Phocaeicola vulgatus | 100% | 0% | 51.25% | 0% |
| Photobacterium profundum | 50% | 0% | 66.67% | 100% |
| Plesiomonas shigelloides | 100% | 33.33% | 89.66% | 0% |
| Proteus mirabilis | 100% | 0% | 42.74% | 0% |
| Proteus penneri | 0% | 0% | 25% | 0% |
| Proteus vulgaris | 18.18% | 0% | 5.56% | 0% |
| Providencia alcalifaciens | 18.18% | 0% | 0% | 0% |
| Providencia heimbachae | 100% | 0% | 71.43% | 0% |
| Providencia rettgeri | 100% | 2.7% | 54.14% | 0% |
| Providencia stuartii | 100% | 0% | 63.64% | 0% |
| Pseudomonas aeruginosa | 35.74% | 0.29% | 29.78% | 6.94% |
| Pseudomonas brassicacearum | 100% | 0% | 92% | 100% |
| Pseudomonas chlororaphis | 100% | 0% | 62.9% | 88.89% |
| Pseudomonas fluorescens | 100% | 0% | 55.22% | 100% |
| Pseudomonas koreensis | 100% | 0% | 91.3% | 100% |
| Pseudomonas mendocina | 100% | 0% | 100% | 0% |
| Pseudomonas monteilii | 100% | 0% | 61.9% | 0% |
| Pseudomonas putida | 100% | 0% | 73.26% | 75% |
| Pseudomonas stutzeri | 100% | 0% | 74.05% | 0% |
| Pseudomonas synxantha | 100% | 0% | 87.5% | 100% |
| Pseudomonas syringae | 100% | 0% | 78.68% | 0% |
| Ralstonia mannitolilytica | 100% | 0% | 100% | 0% |
| Ralstonia pickettii | 100% | 0% | 100% | 0% |
| Ralstonia solanacearum | 95.12% | 27.94% | 42.42% | 0% |
| Raoultella planticola | 100% | 0% | 94.87% | 0% |
| Rhizobium leguminosarum | 100% | 0.78% | 74.65% | 100% |
| Rhodanobacter glycinis | 100% | 0% | 100% | 100% |
| Rhodopseudomonas palustris | 100% | 0% | 100% | 100% |
| Salmonella bongori | 100% | 0% | 89.47% | 0% |
| Salmonella enterica | 67.82% | 1.53% | 58.76% | 2.32% |
| Serratia liquefaciens | 100% | 0% | 50.79% | 0% |
| Serratia marcescens | 100% | 4.52% | 78.24% | 0% |
| Serratia odorifera | 100% | 0% | 100% | 0% |
| Serratia rubidaea | 100% | 0% | 90.91% | 100% |
| Shewanella putrefaciens | 100% | 0% | 87.5% | 0% |
| Sphingobacterium hotanense | 100% | 0% | 100% | 0% |
| Sphingobium yanoikuyae | 100% | 4% | 88.24% | 0% |
| Stenotrophomonas maltophilia | 100% | 0% | 73% | 75% |
| Thalassospira indica | 100% | 0% | 0% | 100% |
| Vibrio alginolyticus | 97.53% | 0% | 25.79% | 0% |
| Vibrio fluvialis | 97.37% | 0% | 32% | 0% |
| Vibrio harveyi | 50% | 0% | 78% | 0% |
| Vibrio neocaledonicus | 100% | 0% | 0% | 0% |
| Vibrio owensii | 83.33% | 0% | 84.21% | 0% |
| Vibrio parahaemolyticus | 50% | 0% | 74.24% | 0% |
| Vibrio vulnificus | 47.27% | 0% | 74.69% | 0% |
| Xanthomonas campestris | 100% | 0% | 38.89% | 0% |
| Yersinia canariae | 100% | 0% | 50% | 0% |
| Yersinia enterocolitica | 100% | 0% | 75.45% | 0% |
| Yersinia kristensenii | 100% | 0% | 65.71% | 0% |
| Yersinia pestis | 100% | 0% | 57.23% | 0% |
| Yersinia pseudotuberculosis | 100% | 0% | 92.65% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 750